US4273871A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Production of angiogenic factor by cell culture
Assignee: Monsanto Co
•
Filing: 1980-03-03
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Granted: 1981-06-16
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Expires: 2000-03-03
Abstract
Abstract Process for the production of human angiogenic factor in vitro comprising growing cells of human foreskin fibroblast cell line on support surface in nutrient culture medium at about 35°-38° C. for a sufficient time to elaborate angiogenic factor and recovering the resulting angiogenic factor from the cells or cell product.
Inventors
- 👤 William R. Tolbert
- 👤 Mary M. Hitt
- 👤 Joseph Feder
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 01:01
CPC Classifications
C; C12; C12P; C12P1/00; C12N; C12N1/00; C12R; C12R2001/00; C12R2001/91; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
50 words
Process for the production of human angiogenic factor in vitro comprising growing cells of human foreskin fibroblast cell line on support surface in nutrient culture medium at about 35°-38° C. for a sufficient time to elaborate angiogenic factor and isolating the resulting angiogenic factor from the cells or cell product.
Claim 2
Dependent Claim
50 words
Process for the production of human angiogenic factor in vitro comprising growing cells of human foreskin fibroblast cell line on support surface in nutrient culture medium at about 35°-38° C. for a sufficient time to elaborate angiogenic factor and isolating the resulting angiogenic factor from the cells or cell product.
Claim 3
Dependent Claim
19 words
The process of claim 1 in which the nutrient culture medium is Dulbecco's modification of Eagle's minimum essential medium.
Claim 4
Dependent Claim
16 words
The process of claim 1 in which the nutrient culture medium is fortified with mammalian serum.
Claim 5
Dependent Claim
17 words
The process of claim 1 in which the angiogenic factor is isolated by extraction from the cells.
Claim 6
Dependent Claim
45 words
The process of claim 1 in which the nutrient culture medium is Dulbecco's modification of Eagle's minimum essential medium and is fortified with fetal bovine serum and in which the angiogenic factor is isolated by extraction from the cells and concentration by carboxymethyl Sephadex chromatography.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-06-15 | 1984-12-15 | 1985-06-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-06-15 | 1988-12-15 | 1989-06-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-06-15 | 1992-12-15 | 1993-06-16 | $7700.00 | Window Closed / Lapsed |