US4275152A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Hydrolysis of protein-bound cholesterol esters
Assignee: Clinical Diagnostic Systems Inc
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Filing: 1979-07-30
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Granted: 1981-06-23
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Expires: 1999-07-30
Abstract
Abstract A novel process is described for hydrolyzing protein-bound cholesterol esters such as are found in blood serum. The method comprises contacting sample containing protein-bound cholesterol esters with a compatible mixture of an enzyme preparation which demonstrates cholesterol ester hydrolase activity and, as an effector, a surfactant which is an alkyl phenoxy polyethoxy ethanol comprising a polyoxyethylene chain of less than about 20 oxyethylene units. Hydrolysis compositions comprising compatible mixtures of an enzyme preparation which demonstrates cholesterol ester hydrolase activity and an effector which is a surfactant as described are also disclosed, as are analytical elements comprising at least one layer which includes such a hydrolysis composition.
Inventors
- 👤 Theodore W. Esders
- 👤 Charles T. Goodhue
- 👤 Christine A. Michrina
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 21:08
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/60; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/911; Y10S435/921; Y10S435/939
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
79 words
A process for hydrolyzing protein-bound cholesterol esters comprising contacting protein-bound cholesterol esters in an aqueous medium in the absence of protease with a compatible mixture of an enzyme preparation having cholesterol ester hydrolase activity and an alkyl phenoxy polyethoxy ethanol comprising a polyoxyethylene chain of less than about 20 oxyethylene units, said mixture containing an amount of said ethanol whereby said compatible mixture is effective to hydrolyze at least 70% of the cholesterol esters available in said aqueous medium.
Claim 2
Dependent Claim
79 words
A process for hydrolyzing protein-bound cholesterol esters comprising contacting protein-bound cholesterol esters in an aqueous medium in the absence of protease with a compatible mixture of an enzyme preparation having cholesterol ester hydrolase activity and an alkyl phenoxy polyethoxy ethanol comprising a polyoxyethylene chain of less than about 20 oxyethylene units, said mixture containing an amount of said ethanol whereby said compatible mixture is effective to hydrolyze at least 70% of the cholesterol esters available in said aqueous medium.
Claim 3
Dependent Claim
80 words
A process for hydrolyzing blood serum cholesterol esters comprising contacting serum containing cholesterol esters in the absence of protease with a compatible mixture of an enzyme preparation having cholesterol ester hydrolase activity and as a surfactant an alkyl phenoxy polyethoxy ethanol comprising a polyoxyethylene chain of less than about 20 oxyethylene units, said mixture containing an amount of said ethanol whereby said compatible mixture is effective to hydrolyze at least 70% of the cholesterol esters available in said aqueous medium.
Claim 4
Dependent Claim
80 words
A process for hydrolyzing blood serum cholesterol esters comprising contacting serum containing cholesterol esters in the absence of protease with a compatible mixture of an enzyme preparation having cholesterol ester hydrolase activity and as a surfactant an alkyl phenoxy polyethoxy ethanol comprising a polyoxyethylene chain of less than about 20 oxyethylene units, said mixture containing an amount of said ethanol whereby said compatible mixture is effective to hydrolyze at least 70% of the cholesterol esters available in said aqueous medium.
Claim 5
Dependent Claim
21 words
The process of claim 2 wherein the enzyme preparation is an esterase preparation derived from a microbial or an animal source.
Claim 6
Dependent Claim
30 words
The process of claim 3 wherein the esterase preparation is derived from a microbial source selected from the group consisting of Candida rugosa, Chromobacterium viscosum, variant paralipolyticum, and Rhizopus arrhizus.
Claim 7
Dependent Claim
14 words
The process of claim 4 wherein the esterase preparation is derived from Candida rugosa.
Claim 8
Dependent Claim
13 words
The process of claim 3 wherein the esterase preparation comprises a pancreatic esterase.
Claim 9
Dependent Claim
23 words
The process of claim 2 wherein the surfactant is an alkyl phenoxy polyethoxy ethanol of from about 7 to about 13 ethoxy units.
Claim 10
Dependent Claim
14 words
The process of claim 7 wherein the surfactant is an octyl phenoxy polyethoxy ethanol.
Claim 11
Dependent Claim
84 words
A process for hydrolyzing protein-bound cholesterol esters comprising contacting protein-bound cholesterol esters in an aqueous medium in the absence of protease with a compatible mixture of microbially derived enzyme preparation having cholesterol ester hydrolase activity and an effector which is an alkyl phenoxy polyethoxy ethanol comprising a polyoxyethylene chain of less than about 20 oxyethylene units, said mixture containing an amount of said ethanol whereby said compatible mixture is effective to hydrolyze at least 70% of the cholesterol esters available in said aqueous medium.
Claim 12
Dependent Claim
84 words
A process for hydrolyzing protein-bound cholesterol esters comprising contacting protein-bound cholesterol esters in an aqueous medium in the absence of protease with a compatible mixture of microbially derived enzyme preparation having cholesterol ester hydrolase activity and an effector which is an alkyl phenoxy polyethoxy ethanol comprising a polyoxyethylene chain of less than about 20 oxyethylene units, said mixture containing an amount of said ethanol whereby said compatible mixture is effective to hydrolyze at least 70% of the cholesterol esters available in said aqueous medium.
Claim 13
Dependent Claim
75 words
A composition for hydrolyzing protein-bound cholesterol esters in an aqueous medium comprising a compatible mixture free from protease and containing an enzyme preparation having cholesterol ester hydrolase activity and as a surfactant an alkyl phenoxy polyethoxy ethanol comprising a polyoxyethylene chain of less than about 20 oxyethylene units, said mixture containing an amount of said ethanol whereby said mixture is effective to hydrolyze at least 70% of the cholesterol esters available in said aqueous medium.
Claim 14
Dependent Claim
75 words
A composition for hydrolyzing protein-bound cholesterol esters in an aqueous medium comprising a compatible mixture free from protease and containing an enzyme preparation having cholesterol ester hydrolase activity and as a surfactant an alkyl phenoxy polyethoxy ethanol comprising a polyoxyethylene chain of less than about 20 oxyethylene units, said mixture containing an amount of said ethanol whereby said mixture is effective to hydrolyze at least 70% of the cholesterol esters available in said aqueous medium.
Claim 15
Dependent Claim
21 words
The composition of claim 10 wherein the enzyme preparation is an esterase preparation derived from a microbial or an animal source.
Claim 16
Dependent Claim
30 words
The composition of claim 11 wherein the esterase preparation is derived from a microbial source selected from the group consisting of Candida rugosa, Chromobacterium viscosum, variant paralipolyticum, and Rhizopus arrhizus.
Claim 17
Dependent Claim
14 words
The composition of claim 12 wherein the esterase preparation is derived from Candida rugosa.
Claim 18
Dependent Claim
13 words
The composition of claim 11 wherein the esterase preparation comprises a pancreatic esterase.
Claim 19
Dependent Claim
23 words
The composition of claim 11 wherein the surfactant is an alkyl phenoxy polyethoxy ethanol of from about 7 to about 13 ethoxy units.
Claim 20
Dependent Claim
83 words
A process for hydrolyzing cholesterol esters in a sample of blood serum comprising contacting said sample in a buffered aqueous medium in the absence of protease with a compatible mixture of an enzyme preparation having cholesterol ester hydrolase activity and an alkyl phenoxy polyethoxyethy ethanol comprising a polyoxyethylene chain of less than about 20 oxyethylene units, said mixture containing an amount of said ethanol whereby said mixture is effective to hydrolyze at least 70% of the cholesterol esters available in said aqueous medium.
Claim 21
Dependent Claim
83 words
A process for hydrolyzing cholesterol esters in a sample of blood serum comprising contacting said sample in a buffered aqueous medium in the absence of protease with a compatible mixture of an enzyme preparation having cholesterol ester hydrolase activity and an alkyl phenoxy polyethoxyethy ethanol comprising a polyoxyethylene chain of less than about 20 oxyethylene units, said mixture containing an amount of said ethanol whereby said mixture is effective to hydrolyze at least 70% of the cholesterol esters available in said aqueous medium.
Claim 22
Dependent Claim
79 words
A composition for hydrolyzing protein-bound cholesterol esters in a sample of an aqueous medium, said composition free from protease and comprising a buffer and a compatible mixture of an enzyme preparation having cholesterol ester hydrolase activity and an alkyl phenoxy polyethoxy ethanol comprising a polyoxyethylene chain of less than about 20 oxyethylene units, said composition containing an amount of said ethanol whereby said composition is effective to hydrolyze at least 70% of the cholesterol esters available in said sample.
Claim 23
Dependent Claim
79 words
A composition for hydrolyzing protein-bound cholesterol esters in a sample of an aqueous medium, said composition free from protease and comprising a buffer and a compatible mixture of an enzyme preparation having cholesterol ester hydrolase activity and an alkyl phenoxy polyethoxy ethanol comprising a polyoxyethylene chain of less than about 20 oxyethylene units, said composition containing an amount of said ethanol whereby said composition is effective to hydrolyze at least 70% of the cholesterol esters available in said sample.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-06-22 | 1984-12-22 | 1985-06-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-06-22 | 1988-12-22 | 1989-06-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-06-22 | 1992-12-22 | 1993-06-23 | $7700.00 | Window Closed / Lapsed |