US4288553A
Expired / Lapsed
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Disopyramide compounds for conjugating proteins used in immunoassays for disopyramide
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1980-02-11
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Granted: 1981-09-08
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Expires: 2000-02-11
Abstract
Abstract Novel carboxyalkylated disopyramides (NORPACE®) are provided as precursors for conjugating proteins, either antigenic for the preparation of antibodies or enzymatic for the preparation of enzyme conjugates, which antibodies and enzyme conjugates find use as reagents in immunoassays. The combination of antibodies and enzyme conjugates provide for sensitive, accurate, rapid assays for disopyramide without interference from closely analogous compounds, such as methadone or disopyramides metabolites.
Inventors
- 👤 Prithipal Singh
- 👤 Pyare L. Khanna
- 👤 Floyd W. Colvin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 13:20
CPC Classifications
C; C07; C07D; C07D213/00; C07D213/02; C07D213/04; C07D213/24; C07D213/54; C07D213/56; C12; C12N; C12N9/00; C12N9/96; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535; Y; Y10; Y10S; Y10S435/00; Y10S435/964; Y10S436/00; Y10S436/811; Y10S436/819; Y10S530/00; Y10S530/806
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
96 words
A compound of the formula: (A--CONHR(CO).sub.m).sub.n Z wherein: A is of the formula: ##STR2## R is a saturated aliphatic linking group of from one to six carbon atoms; Z is hydrogen, hydroxyl, alkoxyl, a group forming an activated ester capable of amide formation in an aqueous medium, or a polypeptide which is antigenic or an enzyme; m is zero or one when Z is a poly(amino acid) and is otherwise one; and n is one when Z is other than a poly(amino acid) and is otherwise one to the molecular weight of Z divided by 500.
Claim 2
Dependent Claim
96 words
A compound of the formula: (A--CONHR(CO).sub.m).sub.n Z wherein: A is of the formula: ##STR2## R is a saturated aliphatic linking group of from one to six carbon atoms; Z is hydrogen, hydroxyl, alkoxyl, a group forming an activated ester capable of amide formation in an aqueous medium, or a polypeptide which is antigenic or an enzyme; m is zero or one when Z is a poly(amino acid) and is otherwise one; and n is one when Z is other than a poly(amino acid) and is otherwise one to the molecular weight of Z divided by 500.
Claim 3
Dependent Claim
17 words
A compound according to claim 1, wherein R is polymethylene of from one to three carbon atoms.
Claim 4
Dependent Claim
10 words
A compound according to claim 2, wherein R is methylene.
Claim 5
Dependent Claim
57 words
A compound of the formula: ACONHR.sup.1 COZ.sup.1 wherein: A is of the formula: ##STR3## R 1 is a saturated aliphatic linking group of from one to three carbon atoms; Z 1 is hydrogen, hydroxyl, alkoxy of from one to six carbon atoms, or a group forming an activated ester capable of amide formation in an aqueous medium.
Claim 6
Dependent Claim
57 words
A compound of the formula: ACONHR.sup.1 COZ.sup.1 wherein: A is of the formula: ##STR3## R 1 is a saturated aliphatic linking group of from one to three carbon atoms; Z 1 is hydrogen, hydroxyl, alkoxy of from one to six carbon atoms, or a group forming an activated ester capable of amide formation in an aqueous medium.
Claim 7
Dependent Claim
11 words
A compound according to claim 4, wherein R 1 is methylene.
Claim 8
Dependent Claim
67 words
A compound of the formula: (ACONHR.sup.2 (CO).sub.m.spsb.2).sub.n.spsb.2 Z.sup.2 wherein: A is of the formula: ##STR4## R 2 is a saturated aliphatic linking group of from one to three carbon atoms; Z 2 is a poly(amino acid), which is antigenic or an enzyme; m 2 is zero or one; n 2 is at least one and not greater than the molecular weight of Z 2 divided by 1,000.
Claim 9
Dependent Claim
67 words
A compound of the formula: (ACONHR.sup.2 (CO).sub.m.spsb.2).sub.n.spsb.2 Z.sup.2 wherein: A is of the formula: ##STR4## R 2 is a saturated aliphatic linking group of from one to three carbon atoms; Z 2 is a poly(amino acid), which is antigenic or an enzyme; m 2 is zero or one; n 2 is at least one and not greater than the molecular weight of Z 2 divided by 1,000.
Claim 10
Dependent Claim
11 words
A compound according to claim 6, wherein R 2 is methylene.
Claim 11
Dependent Claim
29 words
A compound according to claims 6 and 7 wherein m 2 is one, n 2 is in the range of 2 to 500, and Z 2 is an antigen.
Claim 12
Dependent Claim
17 words
A compound according to claim 8 wherein R 2 is methylene and Z 2 is an albumin.
Claim 13
Dependent Claim
29 words
A compound according to claims 6 or 7 wherein m 2 is one, n 2 is in the range of 2 to 20 and Z 2 is an enzyme.
Claim 14
Dependent Claim
12 words
A compound according to claim 10, wherein Z 2 is glucose-6-phosphate dehydrogenase.
Claim 15
Dependent Claim
40 words
Antibodies prepared in response to a compound according to claim 6 wherein m 2 is one, n 2 is in the range of 2 to 500, and z 2 is an antigen, capable of binding to disopyramide, and said compound.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-09-07 | 1985-03-09 | 1985-09-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-09-07 | 1989-03-09 | 1989-09-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-09-07 | 1993-03-09 | 1993-09-08 | $7700.00 | Window Closed / Lapsed |