US4303650A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for production of erythropoietin
Assignee: Ajinomoto Co Inc
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Filing: 1980-10-02
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Granted: 1981-12-01
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Expires: 2000-10-02
Abstract
Abstract The invention provides a novel process for the production of erythropoietin, which is a promising medicine for curing anemia, from human urine containing the same. The inventive method comprises adjusting the pH value of the urine in the range from 6 to 8, if the pH value of the urine is out of this range, and contacting the thus pH-controlled urine with a specific adsorbent so as that the erythropoietin is selectively adsorbed on the adsorbent. The adsorbed erythropoietin is then eluted out by use of an eluant solution to give the product in a partially purified form.
Inventors
- 👤 Kenji Takezawa
- 👤 Hajime Hiratani
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 10:04
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/475; C07K14/505; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/834
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
100 words
A process for the production of purified erythropoietin from human urine which comprises the steps of (a) adjusting the value of pH of the urine in the range from 6 to 8, if the pH of the urine is out of this range, (b) contacting the urine having the thus controlled pH value with an adsorbent selected from the group consisting of a polystyrene-based porous adsorbent resin, chitosan and diatomaceous earth whereby the erythropoietin is adsorbed on the adsorbent, (c) separating the adsorbent having adsorbed the erythropoietin from the depleted urine, and (d) eluting the erythropoietin out of the adsorbent.
Claim 2
Dependent Claim
100 words
A process for the production of purified erythropoietin from human urine which comprises the steps of (a) adjusting the value of pH of the urine in the range from 6 to 8, if the pH of the urine is out of this range, (b) contacting the urine having the thus controlled pH value with an adsorbent selected from the group consisting of a polystyrene-based porous adsorbent resin, chitosan and diatomaceous earth whereby the erythropoietin is adsorbed on the adsorbent, (c) separating the adsorbent having adsorbed the erythropoietin from the depleted urine, and (d) eluting the erythropoietin out of the adsorbent.
Claim 3
Dependent Claim
43 words
The method as claimed in claim 1 wherein the step (a) is preceded by the steps of bringing the pH value of the urine to 7 or higher whereby impurity precipitates are formed in the urine and removing the precipitates from the urine.
Claim 4
Dependent Claim
23 words
The method as claimed in claim 1 wherein the step (d) is carried out using an aqueous alkaline solution as an eluant solution.
References Cited (Backward Citations)
US3865801A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-11-30 | 1985-06-01 | 1985-12-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-11-30 | 1989-06-01 | 1989-12-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-11-30 | 1993-06-01 | 1993-12-01 | $7700.00 | Window Closed / Lapsed |