US4309503A
Expired / Lapsed
US
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Preparation of 11-deoxy anthracycline antibiotics
Assignee: Pfizer Italia SRL
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Filing: 1979-10-05
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Granted: 1982-01-05
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Expires: 1999-10-05
Abstract
Abstract Anthracycling antibiotics of the formula: <IMAGE> wherein R is -CO-CH2OH, -CHOH-CH3, -CO-CH3 or -CH2-CH3 having antitumor and antibacterial activity are prepared by culturing a new mutant strain of microorganism designated as Macromonospora peucetica sp. nova and which has been deposited with the American Type Culture Collection under numbr No. 31366 ATCC.
Inventors
- 👤 Giuseppe Cassinelli
- 👤 Arpad Grein
- 👤 Sergio Merli
- 👤 Giovanni Rivola
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 03:09
CPC Classifications
C; C07; C07H; C07H15/00; C07H15/20; C07H15/24; C07H15/252; C12; C12P; C12P19/00; C12P19/44; C12P19/56
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
78 words
A process for producing an antibiotic complex including a mixture of the compounds having the formula: ##STR4## wherein R is --CO--CH 2 OH, --CHOH--CH 3 , --CO--CH 3 or --CH 2 --CH 3 and a salt thereof, said process comprising cultivating the microorganism Micromonospora peucetica sp. nova under aerobic conditions in an aqueous culture medium containing an assimilable source of carbon, an assimilable source of nitrogen and mineral salts and recovering said antibiotic complex from the culture medium.
Claim 2
Dependent Claim
78 words
A process for producing an antibiotic complex including a mixture of the compounds having the formula: ##STR4## wherein R is --CO--CH 2 OH, --CHOH--CH 3 , --CO--CH 3 or --CH 2 --CH 3 and a salt thereof, said process comprising cultivating the microorganism Micromonospora peucetica sp. nova under aerobic conditions in an aqueous culture medium containing an assimilable source of carbon, an assimilable source of nitrogen and mineral salts and recovering said antibiotic complex from the culture medium.
Claim 3
Dependent Claim
50 words
A process according to claim 1 wherein said cultivating is carried out for 5 to 30 days at a temperature of 25° to 37° C. and at a pH which is initially from 6.5 to 7.0 and which is from 6.5 to 8.0 at the end of the cultivating step.
Claim 4
Dependent Claim
40 words
A process according to claim 1 and further comprising removing the mycelium from the culture medium, separating the culture medium into solid and liquid phases and recovering said antibiotic complex from the mycelium, the solid phase and the liquid phase.
Claim 5
Dependent Claim
21 words
A process according to claim 1 and further comprising isolating from the antibiotic complex the individual glycosides 11-deoxy-adriamycin, 11-deoxy-13-dihydro-daunomycin, 11-deoxy-daunomycin, 11-deoxy-13-deoxo-daunomycin.
Claim 6
Dependent Claim
15 words
A process according to claim 4 wherein isolating is effected by silica gel column chromatography.
Claim 7
Dependent Claim
29 words
A biologically pure culture of the microorganism Micromonospora peucetica sp. nova, having the identifying characteristics of ATCC 31366, said culture being capable of producing anthracycline antibiotics in recoverable quantities.
Claim 8
Dependent Claim
29 words
A biologically pure culture of the microorganism Micromonospora peucetica sp. nova, having the identifying characteristics of ATCC 31366, said culture being capable of producing anthracycline antibiotics in recoverable quantities.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-01-04 | 1985-07-06 | 1986-01-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-01-04 | 1989-07-06 | 1990-01-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-01-04 | 1993-07-06 | 1994-01-05 | $7700.00 | Window Closed / Lapsed |