US4332900A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Construction of co-integrate plasmids from plasmids of Streptomyces and Escherichia
Assignee: Pharmacia and Upjohn Co
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Filing: 1980-10-01
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Granted: 1982-06-01
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Expires: 2000-10-01
Abstract
Abstract Novel chemical compounds, recombinant plasmids pUC1019 and pUC-1020, which are obtained by covalent linkage of ca. 4.2 kb BclI restriction endonuclease fragment of the Streptomyces espinosus plasmid pUC6 into the BamHI endonuclease site of the E. coli plasmid pBR322. Plasmid pUC1024 is obtained by restructuring plasmid pUC1019. These plasmids are useful as cloning vehicles in recombinant DNA work. For example, using DNA methodology, a desired gene, for example, the insulin gene, can be inserted into the plasmids and the resulting plasmids can then be transformed into a suitable host microbe which, upon culturing, produces the desired insulin.
Inventors
- 👤 Jack J. Manis
- 👤 Sarah K. Highlander
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 01:32
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/76; Y; Y10; Y10S; Y10S435/00; Y10S435/82; Y10S435/8215; Y10S435/822; Y10S435/848; Y10S435/849
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
11 words
E. coli CSH50 (pUC1019) having the deposit accession number NRRL B-12252.
Claim 2
Dependent Claim
11 words
E. coli CSH50 (pUC1019) having the deposit accession number NRRL B-12252.
Claim 3
Dependent Claim
11 words
E. coli CSH50 (pUC1020) having the deposit accession number NRRL B-12253.
Claim 4
Dependent Claim
11 words
E. coli CSH50 (pUC1020) having the deposit accession number NRRL B-12253.
Claim 5
Dependent Claim
16 words
Cointegrate plasmid pUC1019, characterized as shown by the restriction map in FIG. 2 of the drawings.
Claim 6
Dependent Claim
16 words
Cointegrate plasmid pUC1019, characterized as shown by the restriction map in FIG. 2 of the drawings.
Claim 7
Dependent Claim
16 words
Cointegrate plasmid pUC1020 characterized as shown by the restriction map in FIG. 3 of the drawings.
Claim 8
Dependent Claim
16 words
Cointegrate plasmid pUC1020 characterized as shown by the restriction map in FIG. 3 of the drawings.
Claim 9
Dependent Claim
53 words
A process for preparing cointegrate plasmids pUC1019 and pUC1020 which comprises: (a) linearizing plasmid pBR322 by BamHI endonuclease to obtain linear plasmid DNA; (b) linearizing plasmid pUC6 by BclI endonuclease to obtain linear plasmid DNA; and (c) ligating said linear plasmid DNA from pBR322 and pUC6 to obtain cointegrate plasmids pUC1019 and pUC1020.
Claim 10
Dependent Claim
53 words
A process for preparing cointegrate plasmids pUC1019 and pUC1020 which comprises: (a) linearizing plasmid pBR322 by BamHI endonuclease to obtain linear plasmid DNA; (b) linearizing plasmid pUC6 by BclI endonuclease to obtain linear plasmid DNA; and (c) ligating said linear plasmid DNA from pBR322 and pUC6 to obtain cointegrate plasmids pUC1019 and pUC1020.
Claim 11
Dependent Claim
71 words
A process for cloning ca. 4.2 kb BclI restriction endonuclease fragment of plasmid pUC6 into a suitable host which comprises: (a) linearizing plasmid pBR322 by BamHI enconuclease to obtain linear plasmid DNA; (b) linearizing plasmid pUC6 by BclI endonuclease to obtain linear plasmid DNA; (c) ligating said linear plasmid DNA from pBR322 and pUC6 to obtain cointegrate plasmids pUC1019 and pUC1020; and, (d) transforming said cointegrate plasmids into said suitable host.
Claim 12
Dependent Claim
71 words
A process for cloning ca. 4.2 kb BclI restriction endonuclease fragment of plasmid pUC6 into a suitable host which comprises: (a) linearizing plasmid pBR322 by BamHI enconuclease to obtain linear plasmid DNA; (b) linearizing plasmid pUC6 by BclI endonuclease to obtain linear plasmid DNA; (c) ligating said linear plasmid DNA from pBR322 and pUC6 to obtain cointegrate plasmids pUC1019 and pUC1020; and, (d) transforming said cointegrate plasmids into said suitable host.
Claim 13
Dependent Claim
13 words
A process, according to claim 6, wherein said suitable host is a bacterium.
Claim 14
Dependent Claim
13 words
A process, according to claim 7, wherein said bacterium is E. coli CSH50.
Claim 15
Dependent Claim
11 words
E. coli RR1 (pUC1024) having the deposit accession number NRRL B-12254.
Claim 16
Dependent Claim
11 words
E. coli RR1 (pUC1024) having the deposit accession number NRRL B-12254.
Claim 17
Dependent Claim
15 words
Plasmid pUC1024, characterized as shown by the restriction map in FIG. 5 of the drawings.
Claim 18
Dependent Claim
15 words
Plasmid pUC1024, characterized as shown by the restriction map in FIG. 5 of the drawings.
Claim 19
Dependent Claim
37 words
A process for preparing plasmid pUC1024 which comprises: (a) digestion of pUC1019 DNA with restriction endonuclease PvuII to obtain fragmented linear plasmid DNA; and, (b) ligating the PvuII fragment of said plasma DNA to obtain plasmid pUC1024.
Claim 20
Dependent Claim
37 words
A process for preparing plasmid pUC1024 which comprises: (a) digestion of pUC1019 DNA with restriction endonuclease PvuII to obtain fragmented linear plasmid DNA; and, (b) ligating the PvuII fragment of said plasma DNA to obtain plasmid pUC1024.
Claim 21
Dependent Claim
49 words
A process for cloning plasmid pUC1024 into a suitable host which comprises: (a) digestion of pUC1019 DNA with restriction endonuclease PvuII to obtain fragmented linear plasmid DNA; (b) ligating the PvuII fragment of said plasmid DNA to obtain plasmid pUC1024; and, (c) transforming said plasmid into said suitable host.
Claim 22
Dependent Claim
49 words
A process for cloning plasmid pUC1024 into a suitable host which comprises: (a) digestion of pUC1019 DNA with restriction endonuclease PvuII to obtain fragmented linear plasmid DNA; (b) ligating the PvuII fragment of said plasmid DNA to obtain plasmid pUC1024; and, (c) transforming said plasmid into said suitable host.
Claim 23
Dependent Claim
13 words
A process, according to claim 12, wherein said suitable host is a bacterium.
Claim 24
Dependent Claim
13 words
A process, according to claim 13, wherein said bacterium is E. coli RR1.
References Cited (Backward Citations)
US4237224A
Cited
US4273875A
Cited
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1985-05-31 | 1985-11-30 | 1986-06-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-05-31 | 1989-11-30 | 1990-06-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-05-31 | 1993-11-30 | 1994-06-01 | $7700.00 | Window Closed / Lapsed |