US4337310A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for the determination of α-amylase
Assignee: Roche Diagnostics GmbH
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Filing: 1980-09-08
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Granted: 1982-06-29
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Expires: 2000-09-08
Abstract
Abstract Method for determining alpha -amylase by incubating with a starch substrate, wherein the substrate is based upon particulate starch derivatized with detectable groups for the determination of alpha -amylase, the substrate consisting of superficially cross-linked starch or amylose grains having a regulated, reduced swellability, said grains having a diameter of from 0.01 to 0.20 mm; these grains are prepared by suspending non-swollen grains in a solution with only enough cross-linking agent to achieve superficial cross-linking and then derivatizing the grains with the detectable groups for the determination of alpha -amylase.
Inventors
- 👤 Hans-Georg Batz
- 👤 Elli Rauscher
- 👤 Gunter Weimann
- 👤 August W. Wahlefeld
- 👤 Wolfgang Gruber
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 19:33
CPC Classifications
C; C08; C08B; C08B31/00; C08B31/003; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/40
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
154 words
Method for the determination of alpha-amylase in serum comprising incubating for a precise period of time of about 15 minutes and at a temperature of about 30° C., the serum suspected of containing alpha-amylase, with a substrate based upon particulate starch derivatized with determinable groups comprising a reactive dyestuff for the determination of alpha-amylase which does not contain a diazo color system, the substrate being a member selected from the group consisting of superficially cross-linked starch grains which are cross-linked with 0.009 to 0.0027 mole of a difunctional cross-linking agent per 100 g non-swollen starch grains and amylose grains which are cross-linked with 0.02 to 0.07 mole of a difunctional cross-linking agent per 100 g of non-swollen amylose grains, said substrate having a regulated, reduced swellability, said starch and amylose grains having a diameter of from 0.01 to 0.20 mm and measuring said determinable groups as a measure of the initial alpha amylase content.
Claim 2
Dependent Claim
154 words
Method for the determination of alpha-amylase in serum comprising incubating for a precise period of time of about 15 minutes and at a temperature of about 30° C., the serum suspected of containing alpha-amylase, with a substrate based upon particulate starch derivatized with determinable groups comprising a reactive dyestuff for the determination of alpha-amylase which does not contain a diazo color system, the substrate being a member selected from the group consisting of superficially cross-linked starch grains which are cross-linked with 0.009 to 0.0027 mole of a difunctional cross-linking agent per 100 g non-swollen starch grains and amylose grains which are cross-linked with 0.02 to 0.07 mole of a difunctional cross-linking agent per 100 g of non-swollen amylose grains, said substrate having a regulated, reduced swellability, said starch and amylose grains having a diameter of from 0.01 to 0.20 mm and measuring said determinable groups as a measure of the initial alpha amylase content.
Claim 3
Dependent Claim
19 words
Method as claimed in claim 1, wherein the starch grains have a diameter of from 0.01 to 0.1 mm.
Claim 4
Dependent Claim
19 words
Method as claimed in claim 1, wherein the amylose grains have a diameter of from 0.05 to 0.20 mm.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-06-28 | 1985-12-28 | 1986-06-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-06-28 | 1989-12-28 | 1990-06-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-06-28 | 1993-12-28 | 1994-06-29 | $7700.00 | Window Closed / Lapsed |