US4338400A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Co-integrate plasmids and their construction from plasmids of Escherichia and Streptomyces
Assignee: Pharmacia and Upjohn Co
•
Filing: 1982-01-26
•
Granted: 1982-07-06
•
Expires: 2002-01-26
Abstract
Abstract Novel chemical compounds, recombinant plasmids pUC1026 and pUC1027, which are obtained by covalent linkage of the E. coli plasmid pBR322 to the Streptomyces espinosus plasmid pUC6. These plasmids are produced by a novel process which can be used to stabilize unstable potential plasmid vectors. These plasmids are useful as cloning vehicles in recombinant DNA work. For example, using DNA methodology, a desired gene, for example, the insulin gene, can be inserted into the plasmids and the resulting plasmids can then be transformed into a suitable host microbe which, upon culturing, produces the desired insulin. The stabilization process disclosed herein can be used to make other stable plasmids.
Inventors
- 👤 Jack J. Manis
- 👤 Sarah K. Highlander
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 02:18
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/67; C12N15/68; C12N15/74; C12N15/76; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/848; Y10S435/886
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
11 words
E. coli RR1 (pUC1026) having the deposit accession number NRRL B-12329.
Claim 2
Dependent Claim
11 words
E. coli RR1 (pUC1026) having the deposit accession number NRRL B-12329.
Claim 3
Dependent Claim
11 words
E. coli RR1 (pUC1027) having the deposit accession number NRRL B-12330.
Claim 4
Dependent Claim
11 words
E. coli RR1 (pUC1027) having the deposit accession number NRRL B-12330.
Claim 5
Dependent Claim
16 words
Recombinant plasmid pUC1026, characterized as shown by the restriction map in FIG. 2 of the drawings.
Claim 6
Dependent Claim
16 words
Recombinant plasmid pUC1026, characterized as shown by the restriction map in FIG. 2 of the drawings.
Claim 7
Dependent Claim
16 words
Recombinant plasmid pUC1027 characterized as shown by the restriction map in FIG. 4 of the drawings.
Claim 8
Dependent Claim
16 words
Recombinant plasmid pUC1027 characterized as shown by the restriction map in FIG. 4 of the drawings.
Claim 9
Dependent Claim
84 words
A process for preparing recombinant plasmid pUC1026 which comprises: (a) digesting plasmid pBR322 with BamHI to obtain linear plasmid DNA; (b) purifying said linear plasmid DNA; (c) digesting plasmid pUC6 with BglII and XhoI and purifying the resulting largest DNA fragment; (d) precipitating said purified DNA's from steps (b) and (c) and ligating to obtain ligated DNA; (e) deproteinizing said ligated sample and then digesting with BclI to obtain linear DNA; and, (f) deproteinizing said linear DNA and ligating to obtain recombinant plasmid pUC1026.
Claim 10
Dependent Claim
84 words
A process for preparing recombinant plasmid pUC1026 which comprises: (a) digesting plasmid pBR322 with BamHI to obtain linear plasmid DNA; (b) purifying said linear plasmid DNA; (c) digesting plasmid pUC6 with BglII and XhoI and purifying the resulting largest DNA fragment; (d) precipitating said purified DNA's from steps (b) and (c) and ligating to obtain ligated DNA; (e) deproteinizing said ligated sample and then digesting with BclI to obtain linear DNA; and, (f) deproteinizing said linear DNA and ligating to obtain recombinant plasmid pUC1026.
Claim 11
Dependent Claim
55 words
A process for preparing recombinant plasmid pUC1027 which comprises: (a) transfering plasmid pUC1013 to E. coli (GM 119), NRRL B-12328; (b) isolating said pUC1013 from E. coli (GM 119), NRRL B-12328; (c) digesting pUC1013 with BclI and purifying the resulting largest DNA fragment; and, (d) ligating the purified DNA fragment to obtain recombinant plasmid pUC1027.
Claim 12
Dependent Claim
55 words
A process for preparing recombinant plasmid pUC1027 which comprises: (a) transfering plasmid pUC1013 to E. coli (GM 119), NRRL B-12328; (b) isolating said pUC1013 from E. coli (GM 119), NRRL B-12328; (c) digesting pUC1013 with BclI and purifying the resulting largest DNA fragment; and, (d) ligating the purified DNA fragment to obtain recombinant plasmid pUC1027.
Claim 13
Dependent Claim
19 words
A process for cloning plasmid pUC1026 into a suitable bacterium which comprises transforming said plasmid into said suitable bacterium.
Claim 14
Dependent Claim
19 words
A process for cloning plasmid pUC1026 into a suitable bacterium which comprises transforming said plasmid into said suitable bacterium.
Claim 15
Dependent Claim
13 words
A process, according to claim 7, wherein said bacterium is E. coli RR1.
Claim 16
Dependent Claim
19 words
A process for cloning plasmid pUC1027 into a suitable bacterium which comprises transforming said plasmid into said suitable bacterium.
Claim 17
Dependent Claim
19 words
A process for cloning plasmid pUC1027 into a suitable bacterium which comprises transforming said plasmid into said suitable bacterium.
Claim 18
Dependent Claim
13 words
A process, according to claim 9, wherein said bacterium is E. coli RR1.
References Cited (Backward Citations)
US4237224A
Cited
US4273875A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-07-05 | 1986-01-04 | 1986-07-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-07-05 | 1990-01-04 | 1990-07-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-07-05 | 1994-01-04 | 1994-07-06 | $7700.00 | Window Closed / Lapsed |