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US4359531A
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US
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Production of isomaltulose
Assignee: Suedzucker AG
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Filing: 1980-10-28
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Granted: 1982-11-16
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Expires: 2000-10-28
Abstract
Abstract Isomaltulose is produced by a process in which at least the isomaltulose-forming enzyme system of an isomaltulose-forming micro-organism genus Erwinia is immobilized on calcium alginate gel and then the immobilized enzyme system is contacted with a sucrose solution to convert at least part of the sucrose to isomaltulose.
Inventors
- 👤 Christopher Bucke
- 👤 Peter S. J. Cheetham
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 01:53
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/10; C12N1/00; C12N1/20; C12N1/205; C12N11/04; C12N9/00; C12N9/10; C12N9/90; C12P; C12P19/00; C12P19/12; C12P19/18; C12P19/24; C12R; C12R2001/00; C12R2001/01; C12R2001/18; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/847; Y10S435/88
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
62 words
A process for the production of isomaltulose, which process consists essentially of immobilizing by entrapment within a calcium alginate gel at least the isomaltulose-forming enzyme system of an isomaltulose-forming micro-organism of the genus Erwinia and then contacting the immobilized enzyme system with a sucrose solution containing at least 30% (w/v) of sucrose to convert at least part of the sucrose to isomaltulose.
Claim 2
Dependent Claim
62 words
A process for the production of isomaltulose, which process consists essentially of immobilizing by entrapment within a calcium alginate gel at least the isomaltulose-forming enzyme system of an isomaltulose-forming micro-organism of the genus Erwinia and then contacting the immobilized enzyme system with a sucrose solution containing at least 30% (w/v) of sucrose to convert at least part of the sucrose to isomaltulose.
Claim 3
Dependent Claim
18 words
The process of claim 1, wherein said enzyme system is immobilized as whole cells of said isomaltulose-forming micro-organism.
Claim 4
Dependent Claim
24 words
The process of claim 1, wherein said enzyme system is immobilized as a solvent extract of whole or disrupted cells of said isomaltulose-forming micro-organism.
Claim 5
Dependent Claim
14 words
The process of claim 1, wherein said micro-organism is of the species E. rhapontici.
Claim 6
Dependent Claim
16 words
The process of claim 7, wherein said micro-organism is of the strain E. rhapontici NCPPB 1578.
Claim 7
Dependent Claim
16 words
The process of claim 1, wherein said sucrose solution contains at least 40% (w/v) of sucrose.
Claim 8
Dependent Claim
15 words
The process of claim 6, wherein said sucrose solution contains about 55% (w/v) of sucrose.
Claim 9
Dependent Claim
17 words
The process of claim 1, wherein from 70 to 95% of said sucrose is converted to isomaltulose.
Claim 10
Dependent Claim
17 words
The process of claim 1, which includes the further step of purifying isomaltulose from the resulting solution.
Claim 11
Dependent Claim
23 words
The process of claim 9, wherein the isomaltulose is crystallized from the resulting solution to yield crystals containing 90% or more of isomaltulose.
Claim 12
Dependent Claim
48 words
The process of claim 1, calcium alginate gel, the isomaltulose-forming micro-organism, the immobilized enzyme system is contacted with a sucrose solution of pH 5-9 containing at least 40% (w/v) of sucrose at 15°-40° C. for a length of time sufficient to convert 80-90% of said sucrose to isomaltulose.
Claim 13
Dependent Claim
25 words
The process of claim 11, wherein said sucrose solution is nutritionally deficient lacking at least one nutrient required for growth of the isomaltulose forming micro-organism.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-11-15 | 1986-05-17 | 1986-11-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-11-15 | 1990-05-17 | 1990-11-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-11-15 | 1994-05-17 | 1994-11-16 | $7700.00 | Window Closed / Lapsed |