US4371614A
Expired / Lapsed
US
⏱️ 0.0 years remaining
E.Coli bacteria carrying recombinant plasmids and their use in the fermentative production of L-tryptophan
Assignee: Ajinomoto Co Inc
•
Filing: 1980-08-22
•
Granted: 1983-02-01
•
Expires: 2000-08-22
Abstract
Abstract A bacterium which comprises a host of the genus Escherichia deficient in the enzyme tryptophanase carrying a plasmid with genetic information to control L-tryptophan production is useful for the fermentative production of L-tryptophan in high yields.
Inventors
- 👤 David M. Anderson
- 👤 Klaus M. Herrmann
- 👤 Ronald L. Somerville
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 20:44
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/22; C12P13/227; C12N; C12N15/00; C12N15/09; C12N15/11; C12N15/52
Claims Analysis
Total Claims: 38
Claim 1
Independent Claim
34 words
A bacterium which comprises a host of the genus Escherichia deficient in the enzyme tryptophanase carrying a plasmid with genetic information to control tryptophan production, wherein said host is resistant to a tryptophan analog.
Claim 2
Dependent Claim
34 words
A bacterium which comprises a host of the genus Escherichia deficient in the enzyme tryptophanase carrying a plasmid with genetic information to control tryptophan production, wherein said host is resistant to a tryptophan analog.
Claim 3
Dependent Claim
44 words
A bacterium which comprises a host of the genus Escherichia deficient in the enzyme tryptophanase carrying a plasmid with genetic information to control tryptophan production, which host additionally carries a gene deficiency selected from the group consisting of trpR, aroP, pheA and combinations thereof.
Claim 4
Dependent Claim
44 words
A bacterium which comprises a host of the genus Escherichia deficient in the enzyme tryptophanase carrying a plasmid with genetic information to control tryptophan production, which host additionally carries a gene deficiency selected from the group consisting of trpR, aroP, pheA and combinations thereof.
Claim 5
Dependent Claim
22 words
The bacterium of claim 1 wherein said analog is selected from the group consisting of 5-methyltryptophan, 5-fluoro-tryptophan, 4-methyl-tryptophan, 4-fluoro-tryptophan, 6-methyl-tryptophan, and 6-fluorotryptophan.
Claim 6
Dependent Claim
16 words
The bacterium of any of claims 1 or 2, wherein said host is a tryptophan auxotroph.
Claim 7
Dependent Claim
16 words
The bacterium of any of claims 1 or 2 wherein said host is a serine auxotroph.
Claim 8
Dependent Claim
13 words
The bacterium of claim 4 wherein said host is also a serine auxotroph.
Claim 9
Dependent Claim
32 words
The bacterium of claim 1 which additionally carries a trpR ts gene alone or in combination with a gene deficiency selected from the group consisting of aroP, tyrA, pheA, and combinations thereof.
Claim 10
Dependent Claim
11 words
The bacterium of claim 2 wherein said gene deficiency is aroP.
Claim 11
Dependent Claim
11 words
The bacterium of claim 2 wherein said gene deficiency is tyrA.
Claim 12
Dependent Claim
11 words
The bacterium of claim 2 wherein said gene deficiency is pheA.
Claim 13
Dependent Claim
12 words
The bacterium of claim 2 wherein said gene deficiency is tyrA, pheA.
Claim 14
Dependent Claim
11 words
The bacterium of claim 2 wherein said gene deficiency is trpR.
Claim 15
Dependent Claim
18 words
The bacterium of claim 2 wherein said gene deficiency is a combination of trpR, tyrA, pheA and aroP.
Claim 16
Dependent Claim
22 words
The bacterium of any of claims 1 or 2 wherein said plasmid is a single copy plasmid or a multi copy plasmid.
Claim 17
Dependent Claim
30 words
The bacterium of any of claims 1 or 2 wherein said plasmid carries a gene selected from the group consisting of serB gene, wild type trp operon and combinations thereof.
Claim 18
Dependent Claim
36 words
The bacterium of any of claims 1 or 2 wherein said plasmid carries a gene selected from the group consisting of a feedback resistant anthranilate synthetase trpE operon gene and combinations thereof with a serB gene.
Claim 19
Dependent Claim
24 words
The bacterium of any of claims 1 or 2 wherein said plasmids also carry a deletion of the attenuator region of the trp operon.
Claim 20
Independent Claim
55 words
A method of producing L-tryptophan by fermentation which comprises: growing a bacterium which comprises a host of the genus Escherichia deficient in the enzyme tryptophanase, carrying a plasmid with genetic information to control tryptophan production in an appropriate growth medium, and collecting tryptophan from said medium; wherein said host is resistant to a tryptophan analog.
Claim 21
Independent Claim
55 words
A method of producing L-tryptophan by fermentation which comprises: growing a bacterium which comprises a host of the genus Escherichia deficient in the enzyme tryptophanase, carrying a plasmid with genetic information to control tryptophan production in an appropriate growth medium, and collecting tryptophan from said medium; wherein said host is resistant to a tryptophan analog.
Claim 22
Independent Claim
67 words
A method of producing L-tryptophan by fermentation which comprises: growing a bacterium which comprises a host of the genus Escherichia deficient in the enzyme tryptophanase carrying a plasmid with genetic information to control tryptophan production in an appropriate growth medium, and collecting tryptophan from said medium; wherein said host additionally carries a gene deficiency selected from the group consisting of trpR, aroP, tyrA, pheA, and combinations thereof.
Claim 23
Independent Claim
67 words
A method of producing L-tryptophan by fermentation which comprises: growing a bacterium which comprises a host of the genus Escherichia deficient in the enzyme tryptophanase carrying a plasmid with genetic information to control tryptophan production in an appropriate growth medium, and collecting tryptophan from said medium; wherein said host additionally carries a gene deficiency selected from the group consisting of trpR, aroP, tyrA, pheA, and combinations thereof.
Claim 24
Dependent Claim
22 words
The method of claim 18 wherein said analog is selected from the group consisting of 5-methyl-tryptophan, 5-fluoro-tryptophan, 4-methyl-tryptophan, 4-fluoro-tryptophan, 6-methyl-tryptophan, and 6-fluoro-tryptophan.
Claim 25
Independent Claim
16 words
The method of any of claims 18 or 19 wherein said host is a tryptophan auxotroph.
Claim 26
Independent Claim
16 words
The method of any of claims 18 or 19 wherein said host is a serine auxotroph.
Claim 27
Dependent Claim
12 words
The method of claim 21 wherein said host is a serine auxotroph.
Claim 28
Dependent Claim
25 words
The method of claim 18 wherein said host additionally carries gene deficiencies selected from the group consisting of trpR, aroP, tyrA, pheA, and combinations thereof.
Claim 29
Dependent Claim
11 words
The method of claim 19 wherein said gene deficiency is aroP.
Claim 30
Dependent Claim
11 words
The method of claim 19 wherein said gene deficiency is tyrA.
Claim 31
Dependent Claim
11 words
The method of claim 19 wherein said gene deficiency is pheA.
Claim 32
Dependent Claim
12 words
The method of claim 19 wherein said gene deficiency is tyrA, pheA.
Claim 33
Dependent Claim
11 words
The method of claim 19 wherein said gene deficiency is trpR.
Claim 34
Dependent Claim
18 words
The method of claim 19 wherein said gene deficiency is a combination of trpR, tyrA, pheA and aroP.
Claim 35
Independent Claim
22 words
The method of any of claims 18 or 19 wherein said plasmid is a single copy plasmid or a multi copy plasmid.
Claim 36
Independent Claim
30 words
The method of any of claims 18 or 19 wherein said plasmid carries a gene selected from the group consisting of serB gene, wild type trp operon and combinations thereof.
Claim 37
Independent Claim
36 words
The method of any of claims 18 or 19 wherein said plasmid carries a gene selected from the group consisting of a feedback resistant anthranilate synthetase trpE operon gene and combinations thereof with a serB gene.
Claim 38
Independent Claim
24 words
The method of any of claims 18 or 19 wherein said plasmids also carry a deletion of the attenuator region of the trp operon.
References Cited (Backward Citations)
US4237224A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-01-31 | 1986-08-02 | 1987-02-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-01-31 | 1990-08-02 | 1991-02-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-01-31 | 1994-08-02 | 1995-02-01 | $7700.00 | Window Closed / Lapsed |