US4376820A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Composition suitable for testing biological tissues and/or liquids, and the method of use
Assignee: Eni SpA
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Filing: 1982-02-23
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Granted: 1983-03-15
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Expires: 2002-02-23
Abstract
Abstract The invention relates to a composition suitable for testing biological tissues and/or liquids, the composition consisting of: (a) a dye chosen from the xanthene, azine, oxazine or acridine series, or from water-soluble dyes of the "diazo" series, or triphenylmethane, (b) a medium compatible with the biological tissue and/or liquid, (c) a substance capable of rapidly de-energizing the dye molecules when it encounters them (i.e. a quencher). The test method using said composition is also described.
Inventors
- 👤 Ivo Giannini
- 👤 Vittorio Baroncelli
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 22:14
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; G; G01; G01N; G01N1/00; G01N1/28; G01N1/30; G01N33/00; G01N33/48; G01N33/50; G01N33/52; C12Q2304/00; C12Q2304/10; G01N2333/00; G01N2333/37; G01N2333/39; G01N2333/395
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
175 words
A method for testing biological tissues and/or liquids, consisting essentially of contacting biological tissue and/or liquid with a composition consisting essentially of a mixture of: (a) a dye at a concentration of between 10 -5 and 10 -4 M and chosen from the xanthene, axine, oxazine or acridine series, or from water-soluble dyes of the "diazo" series, or triphenylmethane, (b) a medium compatible with said biological tissue and/or liquid, and (c) a quencher substance which may be potassium iodide, sodium iodide or the salt of a paramagnetic transition metal and which is capable of de-energising the dye molecules when it encounters them said quencher substance being present in said mixture at a concentration of from 10 -2 to 0.2 M, subjecting the so-treated biological tissue and/or liquid to radiation by a first beam of pulsated light, passing a second beam of monochromatic light through the biological tissue and/or liquid thus treated, and analysing the optical intensity of said second beam as a function of time after it has passed through said biological tissue and/or liquid.
Claim 2
Independent Claim
175 words
A method for testing biological tissues and/or liquids, consisting essentially of contacting biological tissue and/or liquid with a composition consisting essentially of a mixture of: (a) a dye at a concentration of between 10 -5 and 10 -4 M and chosen from the xanthene, axine, oxazine or acridine series, or from water-soluble dyes of the "diazo" series, or triphenylmethane, (b) a medium compatible with said biological tissue and/or liquid, and (c) a quencher substance which may be potassium iodide, sodium iodide or the salt of a paramagnetic transition metal and which is capable of de-energising the dye molecules when it encounters them said quencher substance being present in said mixture at a concentration of from 10 -2 to 0.2 M, subjecting the so-treated biological tissue and/or liquid to radiation by a first beam of pulsated light, passing a second beam of monochromatic light through the biological tissue and/or liquid thus treated, and analysing the optical intensity of said second beam as a function of time after it has passed through said biological tissue and/or liquid.
References Cited (Backward Citations)
US3016292A
Cited
US3298789A
Cited
US3682783A
Cited
US4094745A
Cited
US4174384A
Cited
US4207469A
Cited
US4233402A
Cited
US4219337A
Cited
US4225783A
Cited
US4337063A
Cited
US4225669A
Cited
US4252783A
Cited
US4336029A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-03-14 | 1986-09-13 | 1987-03-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-03-14 | 1990-09-13 | 1991-03-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-03-14 | 1994-09-13 | 1995-03-15 | $7700.00 | Window Closed / Lapsed |