US4404194A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immuno-suppressive substance, its isolation process, and its therapeutic use
Assignee: BERRI-BALZAC A FRENCH BODY CORPORATE
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Filing: 1981-07-21
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Granted: 1983-09-13
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Expires: 2001-07-21
Abstract
Abstract A protein with immuno-suppressive activity and with the following physico-chemical properties: isoelectric point: 4.25, ultraviolet absorption spectrum: as shown in FIG. 1, with a maximum at 260 nm, molecular mass: about 90,000 daltons, can be strained by Coomasie blue, but not by Methylene blue or PAS, desoxyribonuclease, ribonuclease A and neuraminidase proof, and degraded by α, γ and δ chymotrypsins and trypsine.
Inventors
- 👤 Mario Arala-Chaves
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 14:23
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/195; C07K14/37; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/885; Y10S530/00; Y10S530/82; Y10S530/822; Y10S530/825
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
82 words
Protein obtained from the products secreted by microorganisms, having immuno-suppressive activity and the following physico-chemical properties: (1) isoelectric point: 4.25, (2) ultraviolet absorption spectrum: as shown in FIG. 1, with a maximum at 260 nm, (3) molecular mass: about 90,000 daltons as estimated by measuring the migration on a polyacrylamide gel, (4) can be stained by Coomasie blue but not by Methylene blue or PAS, (5) desoxyribonuclease, ribonuclease A and neuraminidase proof and degradable by α, γ and δ chymotrypsins and trypsin.
Claim 2
Dependent Claim
82 words
Protein obtained from the products secreted by microorganisms, having immuno-suppressive activity and the following physico-chemical properties: (1) isoelectric point: 4.25, (2) ultraviolet absorption spectrum: as shown in FIG. 1, with a maximum at 260 nm, (3) molecular mass: about 90,000 daltons as estimated by measuring the migration on a polyacrylamide gel, (4) can be stained by Coomasie blue but not by Methylene blue or PAS, (5) desoxyribonuclease, ribonuclease A and neuraminidase proof and degradable by α, γ and δ chymotrypsins and trypsin.
Claim 3
Dependent Claim
61 words
Process for the production of a protein according to claim 1, comprising subjecting the products secreted by microorganisms whose extracts have immuno-suppressive activity, to a preparative isoelectrofocusing on a saccharose gradient at pH 3.5-6, separating the fractions with immuno-suppressive activity, subjecting these fractions to a preparative isoelectrofocusing on a saccharose gradient at pH 4-5 and isolating the fraction with immuno-suppressive activity.
Claim 4
Dependent Claim
12 words
Process according to claim 2, in which said microorganisms are Streptococcus Intermedius.
Claim 5
Dependent Claim
20 words
Therapeutic composition having immuno-suppressive activity comprising a protein as claimed in claim 1 in admixture with a therapeutically acceptable excipient.
Claim 6
Dependent Claim
24 words
A composition according to claim 4, in unit dosage form, in which said protein is present in the amount of 0.1 to 10 mg.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-09-12 | 1987-03-14 | 1987-09-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-09-12 | 1991-03-14 | 1991-09-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-09-12 | 1995-03-14 | 1995-09-13 | $7700.00 | Window Closed / Lapsed |