US4414334A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Oxygen scavenging with enzymes
Assignee: Phillips Petroleum Co
•
Filing: 1981-08-07
•
Granted: 1983-11-08
•
Expires: 2001-08-07
Abstract
Abstract Removal of ambient oxygen from aqueous liquids is effectively catalyzed by enzymatic deoxygenation systems comprising alcohol oxidase in the presence of alcohol optionally with catalase. Suitable deoxygenation systems described can be used to alleviate corrosion and oxidative degradation in areas such as oil field fluids, circulating water systems, water storage tanks, alcoholic beverages and foodstuffs. As desired, the enzymatic systems can be immobilized on supports or used in solution.
Inventors
- 👤 Donald O. Hitzman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 04:34
CPC Classifications
C; C08; C08J; C08J3/00; C08J3/02; C08J3/03; C09; C09K; C09K8/00; C09K8/02; C09K8/04; C09K8/06; C09K8/14; C09K8/60; C12; C12N; C12N1/00; C12N1/005; C12N9/00; C12N9/0004; C12N9/0006; C12P; C12P1/00; C08J2333/00; C08J2333/24; C08J2333/26; G; G05; G05B; G05B2219/00; G05B2219/10; G05B2219/15; G05B2219/15018; G05B2219/15035; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/938
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
69 words
The process which comprises treating an aqueous liquid containing dissolved oxygen with sufficient alcohol oxidase and an alcohol, selected from the group consisting of straight-chain alcohols of 1 to 4 carbon atoms, effective to substantially eliminate said dissolved oxygen, wherein said aqueous liquid is an oil field fluid containing a polymer selected from the group consisting of polyacrylamide, carboxyalkyl cellulose ethers, biopolysaccharides and starch, thereby stabilizing said polymer solution.
Claim 2
Dependent Claim
69 words
The process which comprises treating an aqueous liquid containing dissolved oxygen with sufficient alcohol oxidase and an alcohol, selected from the group consisting of straight-chain alcohols of 1 to 4 carbon atoms, effective to substantially eliminate said dissolved oxygen, wherein said aqueous liquid is an oil field fluid containing a polymer selected from the group consisting of polyacrylamide, carboxyalkyl cellulose ethers, biopolysaccharides and starch, thereby stabilizing said polymer solution.
Claim 3
Dependent Claim
35 words
The process of claim 1 wherein said alcohol oxidase is derived from the aqueous aerobic fermentation of methanol-utilizing Pichia yeasts selected from the group consisting of Pichia pastoris, Pichia pinus, Pichia trehalophila, and Pichia molischiana.
Claim 4
Dependent Claim
59 words
The process according to claims 1 or 2 wherein said alcohol oxidase is employed as: (a) a whole single-cell protein suspension further containing catalase; (b) a homogenate of ruptured cells further containing catalase; (c) a supernatant derived from centrifugation of (a); (d) a high purity alcohol oxidase by dialysis of (c); or (e) an immobilized form of alcohol oxidase.
Claim 5
Dependent Claim
36 words
The process of claim 3 wherein said alcohol oxidase is employed as said (c), (d), or (e), and further employing sufficient added catalase to convert H 2 O 2 produced by the alcohol oxidase into water.
Claim 6
Dependent Claim
36 words
The process of claim 3 wherein said alcohol oxidase is employed as said (c), (d), or (e), and further employing sufficient added peroxidase to convert H 2 O 2 produced by the alcohol oxidase into water.
Claim 7
Dependent Claim
14 words
The process of claim 3 wherein said oil field fluid is a drilling mud.
Claim 8
Dependent Claim
14 words
The process of claim 6 wherein said oil field fluid is a brine floodwater.
Claim 9
Dependent Claim
14 words
The process of claim 6 wherein said oil field fluid is a surfactant floodwater.
Claim 10
Independent Claim
53 words
A method of stabilizing the viscosity of an aqueous polyacrylamide solution which comprises treating said aqueous polyacrylamide solution with a sufficient amount of alcohol oxidase and an alcohol, selected from the group consisting of straight-chain alcohols of 1 to 4 carbon atoms, effective to substantially eliminate dissolved oxygen in the aqueous polyacrylamide solution.
Claim 11
Independent Claim
53 words
A method of stabilizing the viscosity of an aqueous polyacrylamide solution which comprises treating said aqueous polyacrylamide solution with a sufficient amount of alcohol oxidase and an alcohol, selected from the group consisting of straight-chain alcohols of 1 to 4 carbon atoms, effective to substantially eliminate dissolved oxygen in the aqueous polyacrylamide solution.
Claim 12
Dependent Claim
20 words
The process according to claim 9 wherein said alcohol oxidase is derived from the aqueous fermentation of methanol-utilizing Pichia yeasts.
Claim 13
Dependent Claim
58 words
The process according to claim 10 wherein said alcohol oxidase is employed as: (a) a whole single cell protein suspension further containing catalase; (b) a homogenate of ruptured cells further containing catalase; (c) a supernatant derived from centrifugation of (a); (d) a high purity alcohol oxidase by dialysis of (c); or (e) an immobilized form of alcohol oxidase.
Claim 14
Dependent Claim
36 words
The process of claim 11 wherein said alcohol oxidase is employed as said (c), (d), or (e), and further employing sufficient added catalase to convert H 2 O 2 produced by the alcohol oxidase into water.
Claim 15
Dependent Claim
36 words
The process of claim 11 wherein said alcohol oxidase is employed as said (c), (d), or (e), and further employing sufficient added peroxidase to convert H 2 O 2 produced by the alcohol oxidase into water.
Claim 16
Dependent Claim
88 words
The process which comprises treating an aqueous liquid containing dissolved oxygen with sufficient alcohol oxidase and an alcohol, selected from the group consisting of straight-chain alcohols of 1 to 4 carbon atoms, effective to substantially eliminate said dissolved oxygen, wherein said aqueous liquid is a thickened aqueous polyacrylamide solution and said alcohol oxidase stabilizes the viscosity thereof, and wherein said alcohol oxidase is derived from the aqueous aerobic fermentation of methanol-utilizing Pichia yeasts selected from the group consisting of Pichia pastoris, Pichia pinus, Pichia trehalophila, and Pichia molischiana.
Claim 17
Dependent Claim
88 words
The process which comprises treating an aqueous liquid containing dissolved oxygen with sufficient alcohol oxidase and an alcohol, selected from the group consisting of straight-chain alcohols of 1 to 4 carbon atoms, effective to substantially eliminate said dissolved oxygen, wherein said aqueous liquid is a thickened aqueous polyacrylamide solution and said alcohol oxidase stabilizes the viscosity thereof, and wherein said alcohol oxidase is derived from the aqueous aerobic fermentation of methanol-utilizing Pichia yeasts selected from the group consisting of Pichia pastoris, Pichia pinus, Pichia trehalophila, and Pichia molischiana.
Claim 18
Dependent Claim
57 words
The process according to claim 14 wherein said alcohol oxidase is employed as: (a) a whole single-cell protein suspension further containing catalase; (b) a homogenate of ruptured cells further containing catalase; (c) a supernatant derived from centrifugation of (a); (d) a high purity alcohol oxidase by dialysis of (c); or (e) an immobilized form of alcohol oxidase.
Claim 19
Dependent Claim
36 words
The process of claim 15 wherein said alcohol oxidase is employed as said (c), (d), or (e), and further employing sufficient added catalase to convert H 2 O 2 produced by the alcohol oxidase into water.
Claim 20
Dependent Claim
36 words
The process of claim 15 wherein said alcohol oxidase is employed as said (c), (d), or (e), and further employing sufficient added peroxidase to convert H 2 O 2 produced by the alcohol oxidase into water.
References Cited (Backward Citations)
USRE23523E
Cited
US2752221A
Cited
US2758932A
Cited
US2801697A
Cited
US2940904A
Cited
US3016336A
Cited
US2971851A
Cited
US2971850A
Cited
US3019195A
Cited
US3036305A
Cited
US3005714A
Cited
US3160508A
Cited
US3065193A
Cited
US3163619A
Cited
US3095307A
Cited
US3150086A
Cited
US3234163A
Cited
US3235523A
Cited
US3193393A
Cited
US3326286A
Cited
US3505244A
Cited
US3340930A
Cited
US3343601A
Cited
US3372749A
Cited
US3399725A
Cited
US3770055A
Cited
US3532166A
Cited
US3598181A
Cited
US3650326A
Cited
US3747676A
Cited
US4089789A
Cited
US3800877A
Cited
US4059533A
Cited
US3974066A
Cited
US4096073A
Cited
US4128482A
Cited
US4033896A
Cited
US4042772A
Cited
US4124073A
Cited
US4266034A
Cited
US4250261A
Cited
US4248969A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-11-07 | 1987-05-09 | 1987-11-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-11-07 | 1991-05-09 | 1991-11-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-11-07 | 1995-05-09 | 1995-11-08 | $7700.00 | Window Closed / Lapsed |