US4415546A
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Biologically active analogs of ACTH and radioimmuno assay therefor
Assignee: University of California
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Filing: 1981-05-12
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Granted: 1983-11-15
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Expires: 2001-05-12
Abstract
Abstract A fully steroidogenically active analog of ACTH is disclosed. The analog differs from natural ACTH in that phenylalanine replaces tyrosine in the second position on the peptide chain; and norleucine replaces methionine in the fourth position on the peptide chain. The analog may be iodinated to yield [I]Tyr2-Phe2,Nle4-ACTH, which also retains full steroidogenic activity. When labeled with iodine-125 the analog is useful in following steroidogenic processes and also in radioimmunoassays for ACTH.
Inventors
- 👤 Janakiraman Ramachandran
- 👤 Douglas I. Buckley
- 👤 Donald H. Yamashiro
- 👤 James R. Hagman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 12:31
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; C; C07; C07K; C07K14/00; C07K14/435; C07K14/665; C07K14/695; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
12 words
A steroidogenically active polypeptide and analog of corticotropin having the formula: H-Ser-Phe-Ser-Nle-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Val-Gly-Lys-Lys-Arg-Arg-Pro-Nal-Lys-Val-Tyr-Pro-Asn-Gly-Ala-Glu-Asp-Glu-Ser-Ala-Glu-Ala-Phe-Pro-Leu-Glu-Phe-OH.
Claim 2
Dependent Claim
12 words
A steroidogenically active polypeptide and analog of corticotropin having the formula: H-Ser-Phe-Ser-Nle-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Val-Gly-Lys-Lys-Arg-Arg-Pro-Nal-Lys-Val-Tyr-Pro-Asn-Gly-Ala-Glu-Asp-Glu-Ser-Ala-Glu-Ala-Phe-Pro-Leu-Glu-Phe-OH.
Claim 3
Dependent Claim
21 words
The composition of claim 1 wherein the polypeptide is shortened by one or more amino acid moieties at the -OH end.
Claim 4
Dependent Claim
19 words
The composition of claim 2 wherein the polypeptide is shortened by one amino acid moiety at the -OH end.
Claim 5
Dependent Claim
20 words
The corticortropin analog of claim 1 wherein the polypeptide further includes at least one iodine atom in the polypeptide chain.
Claim 6
Dependent Claim
18 words
The corticotropin analog of claim 4 wherein the iodine atom is attached to the Tyr amino acid moiety.
Claim 7
Dependent Claim
12 words
The corticotropin analog of claim 1 wherein the polypeptide incorporates a radioisotope.
Claim 8
Dependent Claim
17 words
The corticotropin analog of claim 6 wherein the radio-isotope is attached to the Tyr amino acid moiety.
Claim 9
Dependent Claim
11 words
The corticotropin analog of claim 7 wherein the radioisotope is iodine-125.
Claim 10
Dependent Claim
12 words
The corticotropin analog of claim 2 wherein the polypeptide incorporates a radioisotope.
Claim 11
Dependent Claim
17 words
The cortiotropin analog of claim 9 wherein the radio-isotope is attached to the Tyr amino acid moiety.
Claim 12
Dependent Claim
11 words
The corticotropin analog of claim 9 wherein the radio-isotope is iodine-125.
Claim 13
Independent Claim
34 words
A method for investigating the reactive pathways of corticotropin in biological systems comprising substituting a radio-isotope labeled polypeptide, [ 125 I]-Tyr 23 -Phe 2 Nle 4 -ACTH for natural ACTH in the biological system.
Claim 14
Independent Claim
34 words
A method for investigating the reactive pathways of corticotropin in biological systems comprising substituting a radio-isotope labeled polypeptide, [ 125 I]-Tyr 23 -Phe 2 Nle 4 -ACTH for natural ACTH in the biological system.
Claim 15
Dependent Claim
19 words
In an immunological method for assaying ACTH, the step of utilizing a radioisotope labeled ACTH analog as an antigen.
Claim 16
Dependent Claim
19 words
In an immunological method for assaying ACTH, the step of utilizing a radioisotope labeled ACTH analog as an antigen.
Claim 17
Dependent Claim
16 words
The assay method of claim 13 wherein the radio-isotope labeled ACTH analog is labeled with iodine-125.
Claim 18
Dependent Claim
21 words
The method of claim 13 wherein the radio-isotope labeled ACTH analog is [ 125 I]-Tyr 23 -Phe 2 -Nle 4 -ACTH.
Claim 19
Dependent Claim
22 words
The method of claim 13 wherein the radio-isotope labeled ACTH analog is [ 125 I]-Tyr 23 -Phe 2 ,Nle 4 -ACTH (1-38).
Claim 20
Independent Claim
55 words
A method for producing a steroidogenically active peptide analog of ACTH comprising attaching a phenylalaninyl moiety in the second position on the peptide chain between the first and third serinyl moieties and attaching a norleucinyl moiety in the fourth position on the peptide chain between the third serinyl moiety and the fifth glutamic acid moiety.
Claim 21
Independent Claim
55 words
A method for producing a steroidogenically active peptide analog of ACTH comprising attaching a phenylalaninyl moiety in the second position on the peptide chain between the first and third serinyl moieties and attaching a norleucinyl moiety in the fourth position on the peptide chain between the third serinyl moiety and the fifth glutamic acid moiety.
Claim 22
Independent Claim
46 words
A method for producing a radioisotope labeled analog of ACTH comprising reacting a polypeptide Phe 2 ,Nle 4 -ACTH with a salt of iodine-125 to react said salt with said peptide and produce the radio-isotope labeled analog, [ 125 I]-Tyr 23 -Phe 2 ,Nle 4 -ACTH.
Claim 23
Independent Claim
46 words
A method for producing a radioisotope labeled analog of ACTH comprising reacting a polypeptide Phe 2 ,Nle 4 -ACTH with a salt of iodine-125 to react said salt with said peptide and produce the radio-isotope labeled analog, [ 125 I]-Tyr 23 -Phe 2 ,Nle 4 -ACTH.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-11-14 | 1987-05-16 | 1987-11-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-11-14 | 1991-05-16 | 1991-11-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-11-14 | 1995-05-16 | 1995-11-15 | $7700.00 | Window Closed / Lapsed |