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US4427770A
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High glucose-determining analytical element
Assignee: Bayer Corp
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Filing: 1982-06-14
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Granted: 1984-01-24
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Expires: 2002-06-14
Abstract
Abstract An analytical element for quantitatively determining high levels of glucose in blood is made by (a) impregnating a carrier with a first solution, having dissolved therein 4-aminoantipyrine and 3-hydroxy-2,4,6-triiodobenzoic acid or 3,5-dichloro-2-hydroxy-benzene sulfonic acid or salts thereof, a glucose oxidase and a peroxidase, and drying the carrier; and (b) applying to the carrier a second solution of a film-forming agent in a volatile solvent, and drying to remove the volatile solvent and leave a film over the dried first impregnant.
Inventors
- 👤 Shuenn-tzong Chen
- 👤 Mark J. Sherwood
- 👤 Mary E. Warchal
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 18:38
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54; C12Q1/26; C12Q1/28; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/521; G01N33/523; G01N33/525; G01N33/526; C12Q2326/00; C12Q2326/50; C12Q2326/90; C12Q2326/96; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333; Y10T436/144444
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
89 words
A method for preparing an analytical element for determining glucose in a liquid sample which method comprises the steps of (a) impregnating a carrier with a first solution having dissolved therein 4-aminoantipyrine or a salt thereof and 3-hydroxy-2,4,6-triiodobenzoic acid or 3,5-dichloro-2-hydroxy-benzene sulfonic acid, or a salt thereof, a glucose oxidase and a peroxidase, and drying the carrier; and (b) applying to the carrier a second solution of film-forming agent in a volatile solvent, and drying to remove the volatile solvent and leave a film over the dried first impregnant.
Claim 2
Independent Claim
89 words
A method for preparing an analytical element for determining glucose in a liquid sample which method comprises the steps of (a) impregnating a carrier with a first solution having dissolved therein 4-aminoantipyrine or a salt thereof and 3-hydroxy-2,4,6-triiodobenzoic acid or 3,5-dichloro-2-hydroxy-benzene sulfonic acid, or a salt thereof, a glucose oxidase and a peroxidase, and drying the carrier; and (b) applying to the carrier a second solution of film-forming agent in a volatile solvent, and drying to remove the volatile solvent and leave a film over the dried first impregnant.
Claim 3
Dependent Claim
26 words
A method according to claim 1, wherein the concentration of the 4-aminoantipyrine or salt thereof in the first solution is from about 5 to 40 mmoles/liter.
Claim 4
Dependent Claim
31 words
A method according to claim 1, wherein the concentration of the 3-hydroxy-2,4,6-triiodobenzoic acid or 3,5-dichloro-2-hydroxybenzene sulfonic acid, or salt thereof, in the first solution is from about 0.5 to 500 mmoles/liter.
Claim 5
Dependent Claim
21 words
A method according to claim 1, wherein the film-forming agent in the second solution is a hydrophobic cellulose ether or ester.
Claim 6
Dependent Claim
25 words
A method according to claim 1, wherein the first solution contains a buffer for maintaining a pH from about 5 to 8 and a mordant.
Claim 7
Dependent Claim
48 words
A method according to claim 5, wherein the concentration of the 4-aminoantipyrine or salt thereof in the first solution is from about 10 to 30 mmoles/liter and the concentration of the 3,5-dichloro-2-hydroxybenzene sulfonic acid or salt thereof in the first solution is from about 1 to 50 mmoles/liter.
Claim 8
Dependent Claim
56 words
A method according to claim 5, wherein the concentration of the 4-aminoantipyrine or salt thereof in the first solution is about 15 mmoles/liter, the concentration of the 3,5-dichloro-2-hydroxybenzene sulfonic acid or salt thereof in the first solution is about 6 mmoles/liter, and the film-forming agent in the second solution is a hydrophobic cellulose ether or ester.
Claim 9
Dependent Claim
72 words
A method according to claim 5, wherein the 4-aminoantipyrine is present in the first solution as the hydrochloride, the sulfonic acid is present in the first solution as the sodium salt, the solvent in the first solution is water, the pH of the first solution is about 7 and it contains a polymeric polycarboxylic acid or anhydride thereof as the mordant, and the film-forming agent in the second solution is ethyl cellulose.
Claim 10
Dependent Claim
15 words
An analytical element for determining whole blood glucose produced by the process of claim 1.
Claim 11
Dependent Claim
46 words
A method for determining the glucose content of whole blood within the range of about 2000 to 8000 mg/liter comprising contacting the whole blood with an analytical element produced by the process of claim 1, and matching the resulting color of the element with predetermined standards.
Claim 12
Dependent Claim
46 words
A system for determining the glucose content of whole blood over a range from about 0 to 8000 mg/liter comprising, in combination, an element for identifying a glucose content of up to about 2000 mg/liter, and the analytical element produced by the process of claim 1.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-01-23 | 1987-07-25 | 1988-01-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-01-23 | 1991-07-25 | 1992-01-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-01-23 | 1995-07-25 | 1996-01-24 | $7700.00 | Window Closed / Lapsed |