US4439527A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Composition and method for the detection of hydrogen peroxide
Assignee: Roche Diagnostics GmbH
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Filing: 1982-06-07
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Granted: 1984-03-27
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Expires: 2002-06-07
Abstract
Abstract Means and method for the detection of hydrogen peroxide or of hydrogen peroxide-forming substrates, consisting of 4-aminoantipyrine and an aromatic amine or phenol and a neutral to weakly acid buffer as well as other additives and reagents which form H2O2 with the substrate and which are known for use in Trinder's reaction, the 4-aminoantipyrine, in the immiscible state before use, being separate from the acid buffer and from the other reagents if any, and being mixed with a small amount of an alkaline buffer having a pH of 8.5 to 14.0, the two compositions being combined, in a quantity ratio to one another that is suitable for the detection test, in a single package or in a single-use unit.
Inventors
- 👤 Bernd Pakebusch
- 👤 Carsten A. Carstensen
- 👤 Bernward Sojka
- 👤 Hans Lange
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 19:03
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; C12Q2326/00; C12Q2326/90; C12Q2326/96; Y; Y10; Y10S; Y10S436/00; Y10S436/904; Y10T; Y10T436/00; Y10T436/20; Y10T436/206664
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
76 words
Test kit for the detection of hydrogen peroxide or a hydrogen peroxide forming substrate comprising (i) an aromatic amine or phenol and a first buffer which is a neutral to weakly acidic; and (ii) 4-aminoantipyrine, in the immiscible state, separate from said first buffer and mixed with a small amount of a second buffer having a pH of 8.5 to 14.0, the ingredients (i) and (ii) being combined in a quantity ratio appropriate for the test.
Claim 2
Dependent Claim
76 words
Test kit for the detection of hydrogen peroxide or a hydrogen peroxide forming substrate comprising (i) an aromatic amine or phenol and a first buffer which is a neutral to weakly acidic; and (ii) 4-aminoantipyrine, in the immiscible state, separate from said first buffer and mixed with a small amount of a second buffer having a pH of 8.5 to 14.0, the ingredients (i) and (ii) being combined in a quantity ratio appropriate for the test.
Claim 3
Dependent Claim
19 words
Test kit as claimed in claim 1, also containing reagents to form hydrogen peroxide with hydrogen peroxide forming substrates.
Claim 4
Dependent Claim
27 words
Test kit as claimed in claim 1, wherein ingredient (i) contains additional reagents for the formation of H 2 O 2 from a hydrogen peroxide formng substrate.
Claim 5
Dependent Claim
30 words
Test kit as claimed in claim 1, wherein both ingredients (i) and (ii) contain additional reagents for the formation of H 2 O 2 from the hydrogen peroxide forming substrate.
Claim 6
Dependent Claim
80 words
Method of detecting hydrogen peroxide, which method comprises forming a detection composition by combining, shortly prior to use, a 4-aminoantipyrine stabilized with a small amount of a buffer having a pH of 8.5 to 14.0, with an excess of a buffer which is neutral to weakly acidic, and with reagents necessary for Trinder's reaction, contacting a sample with the combined detection composition and evaluation the resultant coloration as a measure of the hydrogen peroxide, or hydrogen peroxide forming substrate, present.
Claim 7
Dependent Claim
80 words
Method of detecting hydrogen peroxide, which method comprises forming a detection composition by combining, shortly prior to use, a 4-aminoantipyrine stabilized with a small amount of a buffer having a pH of 8.5 to 14.0, with an excess of a buffer which is neutral to weakly acidic, and with reagents necessary for Trinder's reaction, contacting a sample with the combined detection composition and evaluation the resultant coloration as a measure of the hydrogen peroxide, or hydrogen peroxide forming substrate, present.
Claim 8
Dependent Claim
33 words
Method as claimed in claim 5 for determining a hydrogen peroxide forming substrate, wherein the combined detection composition also contains a reagent necessary to release hydrogen peroxide from a hydrogen peroxide forming substrate.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-03-27 | 1987-09-26 | 1988-03-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-03-27 | 1991-09-26 | 1992-03-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-03-27 | 1995-09-26 | 1996-03-27 | $7700.00 | Window Closed / Lapsed |