Patent US2475178A successfully refreshed from Google Patents.
US4444880A
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Periodate removal of ascorbate interference in dipsticks for immunoassays
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1982-07-27
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Granted: 1984-04-24
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Expires: 2002-07-27
Abstract
Abstract A solid support "dipstick" immunoassay impregnated with sodium metaperiodate (NaIO4) is described wherein ascorbate interference with an assay having a peroxidase signal producing system is reduced or eliminated.
Inventors
- 👤 Henry Tom
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 11:28
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54393; G01N33/544; G01N33/548; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/962; Y10S435/963; Y10S435/964; Y10S435/97; Y10S436/00; Y10S436/807; Y10S436/81; Y10S436/825; Y10T; Y10T436/00; Y10T436/25; Y10T436/25125; Y10T436/2525
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
95 words
In an immunoassay employing a bibulous support to which is attached a mip and the amount of a mip-peroxidase conjugate which binds to the mip on the support is related to the amount of analyte in a sample, where the peroxidase catalyzes the formation of a dye which binds to said support and ascorbate in said sample interferes with the production of said dye, the improvement which comprises: impregnating said support with a sufficient amount of periodate to reduce said ascorbate interference to a level which does not interfere with the detection of said analyte.
Claim 2
Dependent Claim
95 words
In an immunoassay employing a bibulous support to which is attached a mip and the amount of a mip-peroxidase conjugate which binds to the mip on the support is related to the amount of analyte in a sample, where the peroxidase catalyzes the formation of a dye which binds to said support and ascorbate in said sample interferes with the production of said dye, the improvement which comprises: impregnating said support with a sufficient amount of periodate to reduce said ascorbate interference to a level which does not interfere with the detection of said analyte.
Claim 3
Dependent Claim
40 words
A method according to claim 1, wherein said support impregnated with periodate is further impregnated with a composition comprising a protein and a saccharide in an amount sufficient to stabilize a proteinaceous mip or an enzyme bound to said support.
Claim 4
Dependent Claim
13 words
A method according to claim 2, wherein said composition comprises albumin and sucrose.
Claim 5
Dependent Claim
22 words
A method according to claim 1, wherein an oxidase capable of catalyzing the formation of hydrogen peroxide is bound to said support.
Claim 6
Independent Claim
16 words
A method according to claims 1 or 4, wherein an antibody is bound to said support.
Claim 7
Dependent Claim
94 words
A bibulous support for use in an immunoassay, to which support a mip is attached, wherein the amount of a mip-peroxidase conjugate which binds to the mip on the support is related to the amount of analyte in the sample and wherein the peroxidase catalyzes the formation of a dye which binds to said support and wherein ascorbate in said sample interferes with the production of said dye, said support containing periodate in an amount sufficient to reduce said ascorbate interference to a level which does not interfere with the detection of said analyte.
Claim 8
Dependent Claim
94 words
A bibulous support for use in an immunoassay, to which support a mip is attached, wherein the amount of a mip-peroxidase conjugate which binds to the mip on the support is related to the amount of analyte in the sample and wherein the peroxidase catalyzes the formation of a dye which binds to said support and wherein ascorbate in said sample interferes with the production of said dye, said support containing periodate in an amount sufficient to reduce said ascorbate interference to a level which does not interfere with the detection of said analyte.
Claim 9
Dependent Claim
17 words
The support of claim 6 further containing an oxidase capable of catalyzing the formation of hydrogen peroxide.
Claim 10
Dependent Claim
11 words
The support of claim 6 wherein the mip is an antibody.
Claim 11
Dependent Claim
11 words
The support of claim 7 wherein the mip is an antibody.
Claim 12
Dependent Claim
34 words
The support of claim 6 further containing an amount of a composition comprising a protein and a saccharide in an amount sufficient to stabilize a proteinaceous mip or an enzyme attached to said support.
Claim 13
Dependent Claim
12 words
The support of claim 10 wherein said composition comprises albumin and sucrose.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-04-24 | 1987-10-24 | 1988-04-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-04-24 | 1991-10-24 | 1992-04-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-04-24 | 1995-10-24 | 1996-04-24 | $7700.00 | Window Closed / Lapsed |