US4446235A
Expired / Lapsed
US
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Method for cloning human growth hormone varient genes
Assignee: Genentech Inc
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Filing: 1982-03-22
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Granted: 1984-05-01
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Expires: 2002-03-22
Abstract
Abstract A novel non-allelic human growth hormone variant is disclosed. It is prepared by a new method of obtaining cDNA from the genomic sequences of a eukaryotic organism.
Inventors
- 👤 Peter H. Seeburg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 00:02
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/61; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/102; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/12
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
102 words
A method of obtaining cDNA encoding a desired polypeptide which comprises the steps of: a. probing cloned genomic DNA to obtain genomic sections containing gene for said polypeptide, b. incorporating the genomic sections of step a. into host cells and incubating said host cells under conditions suitable for transcription and processing of the transcript into corresponding mRNA by said host cells, c. creating a cDNA bank containing cDNA encoding said polypeptide from the mRNA of step b., and d. isolating the cDNA for said polypeptide from the cDNA bank of step c. by probing for the requisite DNA sequence of said polypeptide.
Claim 2
Independent Claim
102 words
A method of obtaining cDNA encoding a desired polypeptide which comprises the steps of: a. probing cloned genomic DNA to obtain genomic sections containing gene for said polypeptide, b. incorporating the genomic sections of step a. into host cells and incubating said host cells under conditions suitable for transcription and processing of the transcript into corresponding mRNA by said host cells, c. creating a cDNA bank containing cDNA encoding said polypeptide from the mRNA of step b., and d. isolating the cDNA for said polypeptide from the cDNA bank of step c. by probing for the requisite DNA sequence of said polypeptide.
Claim 3
Dependent Claim
10 words
The method of claim 1 useful for producing HGH-V cDNA.
Claim 4
Dependent Claim
20 words
The method according to claim 1 wherein the genomic sections of step a. are integrated into permissible Cos cell vectors.
References Cited (Backward Citations)
EP0037723A2
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-05-01 | 1987-10-31 | 1988-05-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-05-01 | 1991-10-31 | 1992-05-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-05-01 | 1995-10-31 | 1996-05-01 | $7700.00 | Window Closed / Lapsed |