US4448882A
Expired / Lapsed
US
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Stabilized peroxidase compositions
Assignee: F Hoffmann La Roche AG
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Filing: 1982-07-12
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Granted: 1984-05-15
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Expires: 2002-07-12
Abstract
Abstract A method for the stabilization of peroxidase in a medium containing a serum or serum protein by the addition of 4-amino-antipyrine and stabilized peroxidase compositions are described.
Inventors
- 👤 Hans Brodbeck
- 👤 Harald Gallati
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 11:52
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; C12N; C12N9/00; C12N9/96; C12Q2326/00; C12Q2326/90; C12Q2326/96
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
36 words
A method for the stabilization of peroxidase which is bound to an immunologically active material in a medium containing serum or serum protein, which method comprises adding a stabilizing effective amount of 4-amino-antipyrine to the medium.
Claim 2
Independent Claim
36 words
A method for the stabilization of peroxidase which is bound to an immunologically active material in a medium containing serum or serum protein, which method comprises adding a stabilizing effective amount of 4-amino-antipyrine to the medium.
Claim 3
Dependent Claim
12 words
The method according to claim 1, wherein the peroxidase is horseradish peroxidase.
Claim 4
Dependent Claim
15 words
The method according to claim 1 or claim 2, wherein the serum is goat serum.
Claim 5
Dependent Claim
21 words
The method according to claim 3 wherein 4-amino-antipyrine is added in a concentration of 10 mg. to 1000 mg. per liter.
Claim 6
Dependent Claim
21 words
The method according to claim 4, wherein 4-amino-antipyrine is added in a concentration of 40 mg. to 300 mg. per liter.
Claim 7
Dependent Claim
21 words
The method according to claim 5, wherein 4-amino-antipyrine is added in a concentration of 100 mg. or 200 mg. per liter.
Claim 8
Dependent Claim
24 words
A stabilized comosition of peroxidase which is bound to an immunologically active material comprising said bound peroxidase and a stabilizing effective amount of 4-amino-antipyrine.
Claim 9
Dependent Claim
24 words
A stabilized comosition of peroxidase which is bound to an immunologically active material comprising said bound peroxidase and a stabilizing effective amount of 4-amino-antipyrine.
Claim 10
Dependent Claim
12 words
The composition according to claim 7, wherein the peroxidase is horseradish peroxidase.
Claim 11
Dependent Claim
15 words
The composition according to claim 8 or claim 9, wherein the serum is goat serum.
Claim 12
Dependent Claim
19 words
The composition according to claim 9 wherein the concentration of 4-amino-antipyrine is 10 mg. to 1000 mg. per liter.
Claim 13
Dependent Claim
19 words
The composition according to claim 10, wherein the concentration of 4-amino-antipyrine is 40 mg. to 300 mg. per liter.
Claim 14
Dependent Claim
19 words
The composition according to claim 11, wherein the concentration of 4-amino-antipyrine is 100 mg. or 200 mg. per liter.
References Cited (Backward Citations)
US4378429A
Cited
US4252896A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-05-15 | 1987-11-14 | 1988-05-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-05-15 | 1991-11-14 | 1992-05-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-05-15 | 1995-11-14 | 1996-05-15 | $7700.00 | Window Closed / Lapsed |