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US4450232A
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Incorporation of pyridoxal phosphate in dry analytical elements for the determination of enzymes
Assignee: Clinical Diagnostic Systems Inc
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Filing: 1982-02-04
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Granted: 1984-05-22
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Expires: 2002-02-04
Abstract
Abstract Enzymes which are activated by pyridoxal phosphate are assayed in a dry analytical element which includes a high coverage of pyridoxal phosphate. The coverage of pyridoxal phosphate is such that the multiplication product of the coverage and the spreading coefficient of the element results in a reagent mixture concentration of at least about 0.9 mmole per liter when a liquid sample is contacted with the element thereby eliminating the need for a preincubation of the sample with a solution containing pyridoxal phosphate. The reactions for the determination of the enzymes are initiated by contact of the dry elements with a liquid sample.
Inventors
- 👤 Karl J. Sanford
- 👤 John W. Sutherland
- 👤 Jon N. Eikenberry
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 08:50
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/48; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
101 words
A dry analytical element for the determination of an analyte in a liquid sample wherein said analyte is an enzyme activated by pyridoxal phosphate, said element comprising a support having thereon a layer comprising a reagent composition capable of producing a detectable change in response to the presence of the activated form of said enzyme wherein said element contains a layer comprising pyridoxal phosphate at a coverage such that the multiplication product of said coverage and the spreading coefficient of the element results in a substantially uniform reaction mixture concentration of pyridoxal phosphate of greater than about 0.9 mmole per liter.
Claim 2
Dependent Claim
101 words
A dry analytical element for the determination of an analyte in a liquid sample wherein said analyte is an enzyme activated by pyridoxal phosphate, said element comprising a support having thereon a layer comprising a reagent composition capable of producing a detectable change in response to the presence of the activated form of said enzyme wherein said element contains a layer comprising pyridoxal phosphate at a coverage such that the multiplication product of said coverage and the spreading coefficient of the element results in a substantially uniform reaction mixture concentration of pyridoxal phosphate of greater than about 0.9 mmole per liter.
Claim 3
Dependent Claim
115 words
A dry analytical element for the determination of an analyte in a liquid sample wherein said analyte is an enzyme which is activated by pyridoxal phosphate, said element comprising a support having thereon, in order, a reagent layer and a spreading layer wherein said layers comprise a reagent composition capable of producing a detectable change in response to the presence of the activated form of said enzyme and wherein at least one of said layers comprise pyridoxal phosphate at a coverage such that the multiplication product of said coverage and the spreading coefficient of the element results in a substantially uniform reaction mixture concentration of pyridoxal phosphate of greater than about 0.9 mmole per liter.
Claim 4
Dependent Claim
115 words
A dry analytical element for the determination of an analyte in a liquid sample wherein said analyte is an enzyme which is activated by pyridoxal phosphate, said element comprising a support having thereon, in order, a reagent layer and a spreading layer wherein said layers comprise a reagent composition capable of producing a detectable change in response to the presence of the activated form of said enzyme and wherein at least one of said layers comprise pyridoxal phosphate at a coverage such that the multiplication product of said coverage and the spreading coefficient of the element results in a substantially uniform reaction mixture concentration of pyridoxal phosphate of greater than about 0.9 mmole per liter.
Claim 5
Dependent Claim
121 words
A dry analytical element for the determination of an analyte in a liquid sample wherein said analyte is an enzyme which is activated by pyridoxal phosphate, said element comprising a support having thereon, in order, a reagent layer and an isotropically porous spreading layer wherein said layers comprise a reagent composition capable of producing a detectable change in response to the presence of the activated form of said enzyme wherein said reagent layer comprises a binder and pyridoxal phosphate wherein the coverage of said pyridoxal phosphate is such that the multiplication product of said coverage and the spreading coefficient of the element results in a substantially uniform reaction mixture concentration of pyridoxal phosphate of greater than about 0.9 mmole per liter.
Claim 6
Dependent Claim
121 words
A dry analytical element for the determination of an analyte in a liquid sample wherein said analyte is an enzyme which is activated by pyridoxal phosphate, said element comprising a support having thereon, in order, a reagent layer and an isotropically porous spreading layer wherein said layers comprise a reagent composition capable of producing a detectable change in response to the presence of the activated form of said enzyme wherein said reagent layer comprises a binder and pyridoxal phosphate wherein the coverage of said pyridoxal phosphate is such that the multiplication product of said coverage and the spreading coefficient of the element results in a substantially uniform reaction mixture concentration of pyridoxal phosphate of greater than about 0.9 mmole per liter.
Claim 7
Dependent Claim
37 words
A dry analytical element according to claims 1, 2 or 3 wherein the spreading coefficient and pyridoxal phosphate coverage are such that the reaction mixture concentration of pyridoxal phosphate is greater than about 2.0 mmoles per liter.
Claim 8
Dependent Claim
28 words
A dry analytical element according to claim 1, 2 or 3 wherein said enzyme is aspartate aminotransferase and said detecting reagents comprise NADH, L-asparate, α-ketoglutarate and malate dehydrogenase.
Claim 9
Dependent Claim
30 words
A dry analytical element according to claim 1, 2 or 3 wherein said enzyme is aspartate aminotransferase and said detecting reagents comprise NADH, L-asparate, α-ketoglutarate, lactate dehydrogenase and malate dehydrogenase.
Claim 10
Dependent Claim
27 words
A dry analytical element according to claim 1, 2 or 3 wherein said enzyme is alanine aminotransferase and said reagents comprise NADH, L-alanine, α-ketoglutarate and lactate dehydrogenase.
Claim 11
Independent Claim
75 words
A method for activating an enzyme in a liquid sample with pyridoxal phosphate, said method comprising the step of contacting said sample with a dry element comprising a support having thereon at least one layer comprising pyridoxal phosphate at a coverage such that the multiplication product of said coverage and the spreading coefficient of the element results in a substantially uniform reaction mixture concentration of pyridoxal phosphate is greater than about 0.9 mmole per liter.
Claim 12
Independent Claim
75 words
A method for activating an enzyme in a liquid sample with pyridoxal phosphate, said method comprising the step of contacting said sample with a dry element comprising a support having thereon at least one layer comprising pyridoxal phosphate at a coverage such that the multiplication product of said coverage and the spreading coefficient of the element results in a substantially uniform reaction mixture concentration of pyridoxal phosphate is greater than about 0.9 mmole per liter.
Claim 13
Independent Claim
124 words
A method for the determination of an analyte in a liquid sample wherein said analyte is an enzyme which is activated by pyridoxal phosphate said method comprising the steps of (a) contacting said sample with a dry analytical element comprising a support having thereon a layer comprising a reagent composition capable of producing a detectable change in response to the presence of the activated form of said enzyme said element containing at least one layer comprising pyridoxal phosphate at a coverage such that the multiplication product of said coverage and the spreading coefficient of the element results in a substantially uniform reaction mixture concentration of pyridoxal phosphate is greater than about 0.9 mmole per liter, and (b) determining the rate of said detectable change.
Claim 14
Independent Claim
124 words
A method for the determination of an analyte in a liquid sample wherein said analyte is an enzyme which is activated by pyridoxal phosphate said method comprising the steps of (a) contacting said sample with a dry analytical element comprising a support having thereon a layer comprising a reagent composition capable of producing a detectable change in response to the presence of the activated form of said enzyme said element containing at least one layer comprising pyridoxal phosphate at a coverage such that the multiplication product of said coverage and the spreading coefficient of the element results in a substantially uniform reaction mixture concentration of pyridoxal phosphate is greater than about 0.9 mmole per liter, and (b) determining the rate of said detectable change.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-05-22 | 1987-11-21 | 1988-05-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-05-22 | 1991-11-21 | 1992-05-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-05-22 | 1995-11-21 | 1996-05-22 | $7700.00 | Window Closed / Lapsed |