US4452903A
Expired / Lapsed
US
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Assay method and reagent kit means for lipid-containing body fluid
Assignee: Leeco Diagnostics Inc
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Filing: 1981-02-17
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Granted: 1984-06-05
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Expires: 2001-02-17
Abstract
Abstract A method and reagent kit means are provided for assay of a selected hapten in an aliquot of body fluid containing lipid. The method comprises the steps of constituting the aliquot in a mixture with surfactant and incubating the mixture to solubilize the same. The content of hapten in the incubated mixture is taken up selectively by hapten-specific antibody and read by hapten non-lipid conjugate tracer assay.
Inventors
- 👤 Jin P. Lee
- 👤 Ching Sui A. Yi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 17:35
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/743; G01N33/92; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/808; Y10S436/815; Y10S436/817; Y10S436/826
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
81 words
A storage-stable reagent kit for assay of a selected hapten in an aliquot of body fluid containing lipid, comprising a first container containing a tracer in liquid form including a non-lipid conjugate of said hapten, containers separate from said first container respectively containing first and second competitive binding proteins for immunospecific mutual binding with said hapten and conjugate, and surfactant in said liquid tracer for constituting in an incubation mixture with the aliquot in a quantity sufficient for solubilizing the mixture.
Claim 2
Dependent Claim
81 words
A storage-stable reagent kit for assay of a selected hapten in an aliquot of body fluid containing lipid, comprising a first container containing a tracer in liquid form including a non-lipid conjugate of said hapten, containers separate from said first container respectively containing first and second competitive binding proteins for immunospecific mutual binding with said hapten and conjugate, and surfactant in said liquid tracer for constituting in an incubation mixture with the aliquot in a quantity sufficient for solubilizing the mixture.
Claim 3
Dependent Claim
30 words
A reagent kit according to claim 1 comprising lipase in a quantity sufficient for converting to unbound form any of said hapten present in the incubation mixture in lipid-bound form.
Claim 4
Dependent Claim
12 words
A reagent kit according to claim 1 where the tracer is radioactive.
Claim 5
Dependent Claim
13 words
A reagent kit according to claim 1 where the selected hapten is testosterone.
Claim 6
Dependent Claim
21 words
A reagent kit according to claim 4 where the competitive binding proteins are rabbit anti-human testosterone antiserum and goat anti-rabbit antibody.
Claim 7
Dependent Claim
14 words
A reagent kit according to claim 4 where the conjugate is 125 I-O-(2-iodohistaminylcarboxamidomethyl)testosterone oxime.
Claim 8
Dependent Claim
49 words
A method for assay of a selected hapten in an aliquot of body fluid containing lipid employing a reagent kit according to claim 1, which comprises constituting the aliquot in a mixture comprising said surfactant and incubating the mixture, the quantity of surfactant being sufficient to solubilize the mixture.
Claim 9
Dependent Claim
33 words
A method according to claim 7 which comprises incubating the mixture with lipase in a quantity sufficient for converting to unbound form any of said hapten present in the mixture in lipid-bound form.
Claim 10
Dependent Claim
11 words
A method according to claim 7 where the hapten is testosterone.
Claim 11
Dependent Claim
63 words
A method according to claim 7 which comprises constituting the aliquot in an antibody binding site mixture comprising lipase, surfactant, a tracer which includes a non-lipid conjugate of said hapten, and a first antibody which is specific to said hapten, and incubating the mixture to solubilize the same, convert the bound hapten to unbound hapten and bind unbound hapten with the hapten-specific antibody.
Claim 12
Dependent Claim
11 words
A method according to claim 10 where the hapten is testosterone.
Claim 13
Dependent Claim
13 words
A method according to claim 11 where the conjugate is 125 I-O-(2-iodohistaminylcarboxamidomethyl)testosterone oxime.
Claim 14
Dependent Claim
14 words
A method according to claim 11 where the antibody is rabbit anti-human testosterone antiserum.
Claim 15
Dependent Claim
22 words
A method according to claim 10 comprising incorporating the incubated mixture with a second antibody which is specific to said hapten-specific antibody.
Claim 16
Dependent Claim
15 words
A method according to claim 14 where the first antibody is rabbit anti-human hapten anti-serum.
Claim 17
Dependent Claim
15 words
A method according to claim 14 where the second antibody is goat anti-rabbit hapten antibody.
Claim 18
Dependent Claim
28 words
A method according to claim 14 where the tracer conjugate is radioactive, comprising isolating the resulting radioactive hapten-first antibody-second antibody product and reading the hapten content thereof radiometrically.
Claim 19
Dependent Claim
57 words
A method for assay of a selected hapten in an aliquot of body fluid containing hapten bound to carrier protein, employing a reagent kit according to claim 1, which comprises constituting the aliquot in a mixture comprising said surfactant and incubating the mixture, the quantity of surfactant being sufficient to dissociate the hapten from the carrier protein.
References Cited (Backward Citations)
US3816262A
Cited
US3997470A
Cited
US4148869A
Cited
US4121975A
Cited
US4235866A
Cited
US4208479A
Cited
US4235869A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-06-05 | 1987-12-05 | 1988-06-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-06-05 | 1991-12-05 | 1992-06-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-06-05 | 1995-12-05 | 1996-06-05 | $7700.00 | Window Closed / Lapsed |