US4469797A
Expired / Lapsed
US
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Digoxigenin immunogens, antibodies, labeled conjugates, and related derivatives
Assignee: Bayer Corp
•
Filing: 1982-09-23
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Granted: 1984-09-04
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Expires: 2002-09-23
Abstract
Abstract Immunogen conjugates comprising N-substituted-amino-3-desoxydigoxigenin derivatives coupled to conventional immunogenic carrier materials, and antibodies raised against such conjugates. Also provided are labeled digoxigenin conjugates for use with the digoxigenin antibodies in preferred immunoassay techniques for determining digoxin in biological fluids.
Inventors
- 👤 James P. Albarella
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 13:00
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; C; C07; C07J; C07J41/00; C07J41/0005; G01N33/94; G01N33/9453; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/815; Y10S436/817; Y10S530/00; Y10S530/806
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
54 words
A digoxigenin immunogen conjugate of the formula: ##STR6## wherein Carrier is an immunogenic carrier material, R is a bond or a linking group, one of l and m is one and the other is zero, n is an integer from 2 through 10, and p is on the average from 1 to about 50.
Claim 2
Dependent Claim
54 words
A digoxigenin immunogen conjugate of the formula: ##STR6## wherein Carrier is an immunogenic carrier material, R is a bond or a linking group, one of l and m is one and the other is zero, n is an integer from 2 through 10, and p is on the average from 1 to about 50.
Claim 3
Dependent Claim
21 words
The conjugate of claim 1 wherein R is a bond or a chain comprising between 1 and 20 atoms excluding hydrogen.
Claim 4
Dependent Claim
35 words
The conjugate of claim 1 wherein R is the residue of a bifunctional coupling agent linking the amino or carboxyl terminal group of the digoxigenin moiety to amino or carboxyl groups on the carrier material.
Claim 5
Dependent Claim
18 words
The conjugate of any one of claims 1-3 wherein said carrier material is an immunogenic protein or polypeptide.
Claim 6
Dependent Claim
64 words
A digoxigenin immunogen conjugate of the formula: ##STR7## wherein Carrier is an immunogenic protein or polypeptide Carrier material, Y is an amide group linking carboxyl or amino groups on the carrier material to the digoxigenin moiety, n is an integer from 2 through 10, and p is on the average from 1 to the number of available amide coupling sites on the carrier material.
Claim 7
Dependent Claim
64 words
A digoxigenin immunogen conjugate of the formula: ##STR7## wherein Carrier is an immunogenic protein or polypeptide Carrier material, Y is an amide group linking carboxyl or amino groups on the carrier material to the digoxigenin moiety, n is an integer from 2 through 10, and p is on the average from 1 to the number of available amide coupling sites on the carrier material.
Claim 8
Dependent Claim
16 words
The conjugate of claim 5 wherein p is on the average between 1 and about 50.
Claim 9
Dependent Claim
12 words
The conjugate of claim 5 wherein said carrier material is an albumin.
Claim 10
Dependent Claim
39 words
The conjugate of claim 6 wherein the nitrogen atom in amide group Y is from an amino group in the carrier material and the carbon atom in amide group Y is bonded to the alkylene group in the formula.
Claim 11
Dependent Claim
7 words
The conjugate of claim 8 wherein n=5.
Claim 12
Dependent Claim
9 words
An antibody prepared against the conjugate of claim 1.
Claim 13
Dependent Claim
9 words
An antibody prepared against the conjugate of claim 5.
Claim 14
Dependent Claim
9 words
An antibody prepared against the conjugate of claim 9.
Claim 15
Dependent Claim
16 words
A digoxigenin derivative of the formula: ##STR8## wherein n is an integer from 2 through 10.
Claim 16
Dependent Claim
16 words
A digoxigenin derivative of the formula: ##STR8## wherein n is an integer from 2 through 10.
Claim 17
Dependent Claim
7 words
The derivative of claim 13 wherein n=6.
Claim 18
Dependent Claim
74 words
In an immunoassay method for determining digoxin in a test sample, wherein the test sample is contacted with an antibody to digoxin and with a labeled conjugate comprising digoxin or a binding analog of digoxin and a label, and wherein thereafter the relative amount of the labeled conjugate that becomes bound to said antibody is determined, the improvement which comprises employing the antibody of claim 10, 11 or 12 as the antibody to digoxin.
Claim 19
Dependent Claim
33 words
A test kit for determining digoxin comprising a packaged unit comprising one or more containers holding (a) the antibody of claim 10, 11 or 12, and (b) a labeled digoxin or digoxigenin conjugate.
Claim 20
Dependent Claim
54 words
A test device for determining digoxin by homogeneous, immunoassay, comprising (a) a reagent composition including the antibody of claim 10, 11 or 12 and a labeled digoxin or digoxigenin conjugate which has a detectable property which is altered when bound by said antibody, and (b) a solid carrier member incorporated with said reagent composition.
Claim 21
Dependent Claim
16 words
A β-galactosyl-umbelliferone-digoxigenin conjugate of the formula: ##STR9## and n is an integer from 2 through 10.
Claim 22
Dependent Claim
16 words
A β-galactosyl-umbelliferone-digoxigenin conjugate of the formula: ##STR9## and n is an integer from 2 through 10.
Claim 23
Dependent Claim
7 words
The conjugate of claim 18 wherein n=6.
Claim 24
Dependent Claim
38 words
A flavin adenine dinucleotide-digoxigenin conjugate of the formula: ##STR10## wherein Ribose-(Phos) 2 -Riboflavin represents the riboflavin-pyrophosphate-ribose residue in flavin adenine dinucleotide, r is an integer from 2 through 10, and n is an integer from 2 through 10.
Claim 25
Dependent Claim
38 words
A flavin adenine dinucleotide-digoxigenin conjugate of the formula: ##STR10## wherein Ribose-(Phos) 2 -Riboflavin represents the riboflavin-pyrophosphate-ribose residue in flavin adenine dinucleotide, r is an integer from 2 through 10, and n is an integer from 2 through 10.
Claim 26
Dependent Claim
7 words
The conjugate of claim 20 wherein n=5.
Claim 27
Dependent Claim
9 words
The conjugate of claim 20 or 21 wherein r=6.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-09-04 | 1988-03-05 | 1988-09-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-09-04 | 1992-03-05 | 1992-09-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-09-04 | 1996-03-05 | 1996-09-04 | $7700.00 | Window Closed / Lapsed |