US4473589A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Protein liquefication process and products
Assignee: National By Products Inc
•
Filing: 1982-05-03
•
Granted: 1984-09-25
•
Expires: 2002-05-03
Abstract
Abstract Sources of protein, such as residues and waste products from processing fish, poultry, pork and beef as well as single cell microorganisms, are hydrolyzed to provide liquid products containing substantially all of the component amino acids, lipids and phosphorus in metabolically useful form. The process involves a brief alkaline treatment with heat (120°-170° F.) and alkali (pH 12 or above) which facilitates liquefication and enhances susceptibility to subsequent enzyme hydrolysis with bacterial proteinase at elevated temperatures (100°-140° F.). Cell rupture and protein denaturation occur during alkaline treatment and permit and facilitate the enzyme to rapidly break down the intact proteins to smaller, more soluble molecules. The product may be preserved with various acids at a pH of 3.8 to 4.2.
Inventors
- 👤 Leon D. Freeman
- 👤 James W. Sawhill
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 07:42
CPC Classifications
A; A23; A23J; A23J1/00; A23J1/001; A23J3/00; A23J3/30; A23J3/32; A23J3/34; A23J3/341; A23J3/347
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
109 words
A process for liquefying the protein in a protein source selected from animal tissue and single cell microorganisms comprising the steps of: (a) heating an aqueous slurry of said source at an initial pH above 12 and at a temperature of about 120°-170° F. for a period sufficient to cause cell rupture and protein denaturation and thereby facilitate enzyme hydrolysis of the protein structure to smaller, more soluble molecules; (b) and then hydrolyzing said source to liquefy the protein therein by heating said slurry at about 100°-140° F. at an alkaline pH in the presence of an effective amount of bacterial proteolytic enzyme selected from alkaline and neutral enzymes.
Claim 2
Dependent Claim
109 words
A process for liquefying the protein in a protein source selected from animal tissue and single cell microorganisms comprising the steps of: (a) heating an aqueous slurry of said source at an initial pH above 12 and at a temperature of about 120°-170° F. for a period sufficient to cause cell rupture and protein denaturation and thereby facilitate enzyme hydrolysis of the protein structure to smaller, more soluble molecules; (b) and then hydrolyzing said source to liquefy the protein therein by heating said slurry at about 100°-140° F. at an alkaline pH in the presence of an effective amount of bacterial proteolytic enzyme selected from alkaline and neutral enzymes.
Claim 3
Dependent Claim
45 words
The process for liquefying protein in accordance with claim 1 wherein said step (a) is executed for a time ranging from just bringing the slurry up to 120°-170° F. and immediately proceeding with step (b) to holding the slurry at 120°-170° F. for 120 minutes.
Claim 4
Dependent Claim
46 words
The process for liquefying protein in accordance with claim 1 wherein said step (a) is executed in about 5-60 minutes at a temperature of about 140°-160° F. and a pH of about 12-13 and said step (b) is executed at a temperature of about 115°-125° F.
Claim 5
Dependent Claim
20 words
The process for liquefying protein in accordance with claim 3 wherein said step (a) is executed in about 20-40 minutes.
Claim 6
Dependent Claim
26 words
The process for liquefying protein in accordance with claim 2 wherein said steps (a) and (b) together are executed in not more than about 24 hours.
Claim 7
Dependent Claim
38 words
The process for liquefying protein in accordance with claim 1 wherein the enzyme of step (b) is a high alkali protease and is added at the end of step (a) at the pH then existing in the slurry.
Claim 8
Dependent Claim
43 words
The process for liquefying protein in accordance with claim 1 wherein the pH is lowered at the end of step (a) by the addition of mineral acid before adding the enzyme of step (b) to a pH which facilitates hydrolysis by said enzyme.
Claim 9
Dependent Claim
33 words
The process for liquefying protein in accordance with claim 7 wherein the pH is lowered to about 8-9 with the addition of an acid selected from hydrochloric acid, phosphoric acid and sulfuric acid.
Claim 10
Dependent Claim
20 words
The process for liquefying protein in accordance with claim 7 wherein the enzyme of step (b) is a neutral protease.
Claim 11
Dependent Claim
50 words
The process for liquefying protein in accordance with claim 6 wherein a plurality of enzymes are successively introduced as hydrolysis of said source progresses, each successive enzyme added being selected for its activity at successively lowered pH's of the slurry caused by increased liberation of carboxyl groups of the protein.
Claim 12
Dependent Claim
49 words
The process for liquefying protein in accordance with claim 1 wherein the pH of step (a) is adjusted to about 12-13 by the addition of about 1-5 weight percent of said source of a base relative to said aqueous slurry selected from sodium hydroxide, potassium hydroxide and ammonium hydroxide.
Claim 13
Dependent Claim
29 words
The process for liquefying protein in accordance with claim 1 wherein the enzyme of step (b) is present in an amount of about 0.1-1.0 weight percent of said source.
Claim 14
Dependent Claim
26 words
The process for liquefying protein in accordance with claim 1 and including the further step (c) of adding a preservative to the hydrolysate of step (b).
Claim 15
Dependent Claim
64 words
The process for liquefying protein in accordance with claim 12 wherein the pH of the hydrolysate of step (b) is adjusted to about 4.2 or less and said preservative comprises about 0.05-1.0 weight percent of solids of at least one substantially nontoxic carboxylic acid or substantially nontoxic salt thereof, said carboxylic acid being selected from solid unsaturated aliphatic carboxylic acids and aromatic carboxylic acids.
References Cited (Backward Citations)
US2431256A
Cited
US2477255A
Cited
US3578461A
Cited
US3924005A
Cited
US3891770A
Cited
US4036993A
Cited
US4081555A
Cited
US4220723A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-09-25 | 1988-03-26 | 1988-09-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-09-25 | 1992-03-26 | 1992-09-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-09-25 | 1996-03-26 | 1996-09-25 | $7700.00 | Window Closed / Lapsed |