US4479895A
Expired / Lapsed
US
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Immunoglobulin half-molecules and process for producing hybrid antibodies
Assignee: Dade Behring Inc
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Filing: 1982-05-05
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Granted: 1984-10-30
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Expires: 2002-05-05
Abstract
Abstract A process for preparing covalent hybrid antibodies is provided. These antibodies are obtained by the ordered covalent hybridization of intact immunoglobulin molecules. The generation of half-molecules from different IgG antibodies by reductive or S-sulfonation selective cleavage is followed by the ordered association of the different half-molecules. The pure covalent hybrid antibodies as new substances are also provided along with IgG half-molecules.
Inventors
- 👤 Karen Auditore-Hargreaves
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 14:21
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/68; G01N33/53; G01N33/531; G01N33/577; G01N33/68; G01N33/6854; Y; Y10; Y10S; Y10S530/00; Y10S530/806; Y10S530/866
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
26 words
A substantially pure immunoglobulin heavy chain-light chain half-molecule having the structure R--S--X, where R is H 1 L 1 and X is SO 3 - .
Claim 2
Dependent Claim
26 words
A substantially pure immunoglobulin heavy chain-light chain half-molecule having the structure R--S--X, where R is H 1 L 1 and X is SO 3 - .
Claim 3
Dependent Claim
12 words
The immunoglobulin half-molecule of claim 1 having antigen-binding specificity for glucose oxidase.
Claim 4
Dependent Claim
12 words
The immunoglobulin half-molecule of claim 1 having antigen-binding specificity for horseradish peroxidase.
Claim 5
Dependent Claim
21 words
The immunoglobulin half-molecule of claim 1 having antigen-binding specificity for digoxin, wherein the half-molecule is obtained from monoclonal antibody to digoxin.
Claim 6
Dependent Claim
21 words
The immunoglobulin half-molecule of claim 1 having antigen-binding specificity for theophylline, wherein the half-molecule is obtained from monoclonal antibody to theophylline.
Claim 7
Dependent Claim
57 words
A process for preparing S-sulfonated immunoglobulin half-molecules by selectively cleaving an immunoglobulin molecule into its heavy chain-light half-molecules by sulfitolysis of the interheavy chain disulfide linkage; wherein the sulfitolysis reaction comprises the steps of: (A) mixing an immunoglobulin molecule with sodium sulfite and 5,5'-dithiobis(2-nitrobenzoic acid); (B) incubating in a nonoxidizing atmosphere; and (C) separating the protein products.
Claim 8
Dependent Claim
57 words
A process for preparing S-sulfonated immunoglobulin half-molecules by selectively cleaving an immunoglobulin molecule into its heavy chain-light half-molecules by sulfitolysis of the interheavy chain disulfide linkage; wherein the sulfitolysis reaction comprises the steps of: (A) mixing an immunoglobulin molecule with sodium sulfite and 5,5'-dithiobis(2-nitrobenzoic acid); (B) incubating in a nonoxidizing atmosphere; and (C) separating the protein products.
Claim 9
Dependent Claim
65 words
A process for preparing reduced immunoglobulin half-molecules by selectively cleaving an immunoglobulin molecule into its heavy chain-light chain half-molecules by sulfitolysis of the inter-heavy chain disulfide linkage followed by reduction of the S-sulfonated half-molecules wherein the sulfitolysis reaction comprises the steps of: (A) mixing an immunoglobulin molecule with sodium sulfite and 5,5'-dithiobis(2-nitrobenzoic acid); (B) incubating in a nonoxidizing atmosphere; and (C) separating the protein products.
Claim 10
Dependent Claim
65 words
A process for preparing reduced immunoglobulin half-molecules by selectively cleaving an immunoglobulin molecule into its heavy chain-light chain half-molecules by sulfitolysis of the inter-heavy chain disulfide linkage followed by reduction of the S-sulfonated half-molecules wherein the sulfitolysis reaction comprises the steps of: (A) mixing an immunoglobulin molecule with sodium sulfite and 5,5'-dithiobis(2-nitrobenzoic acid); (B) incubating in a nonoxidizing atmosphere; and (C) separating the protein products.
Claim 11
Dependent Claim
13 words
The process of claim 7 wherein the reduction step utilizes a thiol-containing reagent.
Claim 12
Dependent Claim
20 words
The process of claim 8 wherein the thiol-containing reagent is selected from the group consisting of β-mercaptoethanol, dithiothreitol and β-mercaptoethylamine.
Claim 13
Dependent Claim
23 words
The process of claims 7, 8 or 9 carried out in a nonoxidizing atmosphere and in the presence of alkali earth metal salts.
Claim 14
Dependent Claim
37 words
A process for preparing reduced immunoglobulin half-molecules by selectively cleaving an immunoglobulin molecule into its heavy chain-light chain half-molecules by reduction of the inter-heavy chain disulfide linkage; wherein the immunoglobulin molecule is bound to an immobilized receptor.
Claim 15
Dependent Claim
37 words
A process for preparing reduced immunoglobulin half-molecules by selectively cleaving an immunoglobulin molecule into its heavy chain-light chain half-molecules by reduction of the inter-heavy chain disulfide linkage; wherein the immunoglobulin molecule is bound to an immobilized receptor.
Claim 16
Dependent Claim
15 words
The process of claim 11 wherein the receptor is an antigen bonded to a support.
Claim 17
Dependent Claim
15 words
The process of claim 11 wherein the receptor is Protein A bonded to a support.
Claim 18
Dependent Claim
16 words
The process of claims 11, 12 or 13 wherein the reduction step utilizes a thiol-containing reagent.
Claim 19
Dependent Claim
20 words
The process of claim 14 wherein the thiol-containing reagent is selected from the group consisting of β-mercaptoethanol, dithiothreitol and β-mercaptoethylamine.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-10-30 | 1988-04-30 | 1988-10-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-10-30 | 1992-04-30 | 1992-10-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-10-30 | 1996-04-30 | 1996-10-30 | $7700.00 | Window Closed / Lapsed |