US4490464A
Expired / Lapsed
US
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Electrode for the electrochemical regeneration of coenzyme, a method of making said electrode, and the use thereof
Assignee: Cambridge Sensors Ltd
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Filing: 1982-03-08
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Granted: 1984-12-25
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Expires: 2002-03-08
Abstract
Abstract Electrodes for the electrochemical regeneration of the co-enzymes, NADH, NADPH or analogs thereof. The electrode surface has been modified by adsorbing thereto a condensed aromatic ring system comprising at least three, preferably four or more, condensed aromatic rings. A method of preparing the electrodes is also described, in which a condensed aromatic ring system is adsorbed to the surface of carbon or a graphitic material. Finally, there is described the use of said electrodes for the electrochemical regeneration of co-enzyme in biochemical, microbiological or biochemical processes as the anode in biochemical fuel cells or for analysis in systems utilizing co-enzyme-dependent enzyme.
Inventors
- 👤 Lo G. Gorton
- 👤 Hans Å. Jaegfeldt
- 👤 Arne B. Torstensson
- 👤 Gillis R. Johansson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 06:11
CPC Classifications
C; C12; C12P; C12P1/00; C25; C25B; C25B11/00; C25B11/04; C25B11/042; C25B11/043; C25B3/00; C25B3/01; C25B3/07; C25B3/09; H; H01; H01M; H01M4/00; H01M4/86; H01M4/90; H01M4/96; H01M8/00; H01M8/16; Y; Y02; Y02E; Y02E60/00; Y02E60/30; Y02E60/50; Y02P; Y02P70/00; Y02P70/50; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
53 words
An electrode for the electrochemical regeneration of coenzymes dihydronicotinamide adenine dinucleotide (NADH), dihydronicotinamide adenine dinucleotide phosphate (NADPH), or analogs thereof, characterized in that it comprises carbon or a graphitic material to the surface of which is adsorbed a condensed aromatic ring system comprising phenoxazinium ions, phenoxazinones, phenothiazinium ions or phenothiazinones, substituted or unsubstituted.
Claim 2
Dependent Claim
53 words
An electrode for the electrochemical regeneration of coenzymes dihydronicotinamide adenine dinucleotide (NADH), dihydronicotinamide adenine dinucleotide phosphate (NADPH), or analogs thereof, characterized in that it comprises carbon or a graphitic material to the surface of which is adsorbed a condensed aromatic ring system comprising phenoxazinium ions, phenoxazinones, phenothiazinium ions or phenothiazinones, substituted or unsubstituted.
Claim 3
Dependent Claim
63 words
An electrode as claimed in claim 1, characterized in that one or more enzymes, at least one of which is of the dehydrogenase type, are present in dissolved, suspended or immobilized form on or at the electrode surface, an that NADH or NADPH or analogs thereof have been applied in dissolved or immobilized form, whereby said coenzyme can be reoxidized by the electrode.
Claim 4
Dependent Claim
29 words
An electrode as claimed in claim 2, characterized in that the enzyme or enzymes are retained in the vicinity of said electrode by a membrane permeable to small molecules.
Claim 5
Dependent Claim
47 words
An electrode as claimed in claim 1, characterized in that one or more enzymes, at least one of which is of the dehydrogenase type, have been immobilized and applied such that the coenzymes or coenzyme analogs can be conveyed between the enzyme bed and the electrode surface.
Claim 6
Dependent Claim
47 words
An electrode as claimed in claim 1, characterized in that the active ring system on the electrode surface can be replenished from reservoirs in or on the electrode material, or from a solution of the substances or on the ions in the working electrolyte in low contents.
Claim 7
Independent Claim
61 words
A method of making an electrode for the electrochemical regeneration of the coenzymes dihydroicotinamide adenine dinucleotide (NADH), dihydronicotinamide adenine dinucleotide phosphate (NADPH), or analogs thereof, characterized in that there is adsorbed to the surface of the electrode which consists of carbon or a graphitic material a condensed aromatic ring system comprising phenoxazinium ions, phenoxasinones, phenothiazinium ions or phenothiazinones, substituted or unsubstituted.
Claim 8
Independent Claim
61 words
A method of making an electrode for the electrochemical regeneration of the coenzymes dihydroicotinamide adenine dinucleotide (NADH), dihydronicotinamide adenine dinucleotide phosphate (NADPH), or analogs thereof, characterized in that there is adsorbed to the surface of the electrode which consists of carbon or a graphitic material a condensed aromatic ring system comprising phenoxazinium ions, phenoxasinones, phenothiazinium ions or phenothiazinones, substituted or unsubstituted.
Claim 9
Dependent Claim
74 words
In a method of electrochemical regeneration of coenzymes for biotechnical, microbiological, or biochemical purposes, the steps of providing an electrode comprising carbon or a graphitic material to the surface of which is adsorbed a condensed aromatic ring system comprising phenoxazinium ions, phenoxazinones, phenothiazinium ions or phenothiazinones, substituted or unsubstituted, and employing said electrode in an electrochemical cell for the regeneration of coenzymes dihydronicotinamide adenine dinucleotide (NADH), dihydroniciotinamide adenine dinucleotide phosphate (NADPH), or analogs thereof.
Claim 10
Dependent Claim
74 words
In a method of electrochemical regeneration of coenzymes for biotechnical, microbiological, or biochemical purposes, the steps of providing an electrode comprising carbon or a graphitic material to the surface of which is adsorbed a condensed aromatic ring system comprising phenoxazinium ions, phenoxazinones, phenothiazinium ions or phenothiazinones, substituted or unsubstituted, and employing said electrode in an electrochemical cell for the regeneration of coenzymes dihydronicotinamide adenine dinucleotide (NADH), dihydroniciotinamide adenine dinucleotide phosphate (NADPH), or analogs thereof.
Claim 11
Independent Claim
71 words
A method of improving the performance of a biochemical fuel cell which operates with dehydrogenases as catalysts and with coenzyme as energy-transferring redox pair, which comprises providing an electrode comprising carbon or a graphitic material to the surface of which is adsorbed a condensed aromatic ring system comprising phenoxazinium ions, phenoxazinones, phenothiazinium ions or phenothiazinones, substituted or unsubstituted, and operating said fuel cell with said electrode serving as the anode thereof.
Claim 12
Independent Claim
71 words
A method of improving the performance of a biochemical fuel cell which operates with dehydrogenases as catalysts and with coenzyme as energy-transferring redox pair, which comprises providing an electrode comprising carbon or a graphitic material to the surface of which is adsorbed a condensed aromatic ring system comprising phenoxazinium ions, phenoxazinones, phenothiazinium ions or phenothiazinones, substituted or unsubstituted, and operating said fuel cell with said electrode serving as the anode thereof.
Claim 13
Independent Claim
68 words
A method of analysis in systems utilizing coenzyme-dependent enzyme, the comprises providing an electrode comprising carbon or a graphitic material to the surface of which is adsorbed a condensed aromatic ring system comprising phenoxazinum ions, phenoxazinones, phenothiazinium ions or phenothiazinones, substituted or unsubstituted, and employing said electrode in an analytical apparatus in which coenzymes dihydronicotinamide adenine dinucleotide (NADH), dihydronicotinamide adenine dinucleotide phosphate (NADPH), or analogs thereof are generated.
Claim 14
Independent Claim
68 words
A method of analysis in systems utilizing coenzyme-dependent enzyme, the comprises providing an electrode comprising carbon or a graphitic material to the surface of which is adsorbed a condensed aromatic ring system comprising phenoxazinum ions, phenoxazinones, phenothiazinium ions or phenothiazinones, substituted or unsubstituted, and employing said electrode in an analytical apparatus in which coenzymes dihydronicotinamide adenine dinucleotide (NADH), dihydronicotinamide adenine dinucleotide phosphate (NADPH), or analogs thereof are generated.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-12-25 | 1988-06-25 | 1988-12-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-12-25 | 1992-06-25 | 1992-12-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-12-25 | 1996-06-25 | 1996-12-25 | $7700.00 | Window Closed / Lapsed |