US4515714A
Expired / Lapsed
US
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Method for purification of hepatitis B virus surface antigen
Assignee: Chemo Sero Therapeutic Research Institute Kaketsuken
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Filing: 1984-03-06
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Granted: 1985-05-07
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Expires: 2004-03-06
Abstract
Abstract An improved method for the purification of hepatitis B virus surface antigen by column chromatography, which is characteristic in using as a gel for the chromatography a sulfuric acid ester of a crosslinked polysaccharide or cellulose which is obtained by sulfating a crosslinked polysaccharide or cellulose. Said method can give a highly purified hepatitis B virus surface antigen which is useful for the preparation of hepatitis B virus vaccine.
Inventors
- 👤 Tetsuo Kawahara
- 👤 Hiroshi Mizokami
- 👤 Kyosuke Mizuno
- 👤 Sadao Susumi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 17:15
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; A; A61; A61K; A61K39/00; A61K39/12; A61K39/29; C12; C12N; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10122; Y; Y10; Y10S; Y10S530/00; Y10S530/806; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
67 words
In a method for the purification of hepatitis B virus surface anitgen comprising subjecting a heptitis B virus surface anitgen-containing solution to column chromatography, the improvement comprising using a sulfuric acid ester of a cross-linked polysaccharide or cellulose as a gel for the chromatography, said sulfuric acid ester being prepared by treating a gel of crosslinked polysaccharide or cellulose with a sulfating agent in an organic solvent.
Claim 2
Dependent Claim
67 words
In a method for the purification of hepatitis B virus surface anitgen comprising subjecting a heptitis B virus surface anitgen-containing solution to column chromatography, the improvement comprising using a sulfuric acid ester of a cross-linked polysaccharide or cellulose as a gel for the chromatography, said sulfuric acid ester being prepared by treating a gel of crosslinked polysaccharide or cellulose with a sulfating agent in an organic solvent.
Claim 3
Dependent Claim
60 words
The method according to claim 1, wherein the sulfuric acid ester of a crosslinked polysaccharide is a member selected from the group consisting of a crosslinked celluluse sulfate, a crosslinked agarose sulfate, and a crosslinked dextran sulfate, which is prepared by treating a gel of crosslinked cellulose, crosslinked agarose or crosslinked dextran with a sulfating agent in an organic solvent.
Claim 4
Dependent Claim
34 words
The method according to claim 2, wherein the cross-linked cellulose sulfate is an epichlorohydrin-crossed-linked cellulose sulfate which is prepared by treating a gel of epichlorohydrin-crosslinked cellulose with a sulfating agent in an organic solvent.
Claim 5
Dependent Claim
33 words
The method according to claim 2, wherein the cross-linked agarose sulfate is an epichlorohydrin-crosslinked agarose sulfate which is prepared by treating a gel of epichlorohydrin-crosslinked agarose with sulfating agent in an organic solvent.
Claim 6
Dependent Claim
33 words
The method according to claim 2, wherein the cross-linked dextran sulfate is an epichlorohydrin-crosslinked dextran sulfate which is prepared by treating a gel of ephichlorohydrin-crosslinked dextran with sulfating agent in an organic solvent.
Claim 7
Dependent Claim
53 words
The method according to claim 1, wherein the sulfuric acid ester of cellulose is a sulfuric acid ester of a cellulose selected from crystalline cellulose and a cellulose having a crystalline area and onocrystalline area, which is prepared by treating a gel of the cellulose with a sulfating agent in an organic solvent.
Claim 8
Independent Claim
128 words
A method for the purification of hepatitis B virus surface antigen, which comprises packing a gel of a sulfuric acid ester of a crosslinked polysaccharide or cellulose within a column, equilibrating the column with a buffer solution of an ionic strength of 0.001 to 1.0 and a pH range of 4 to 10, passing through the column a hepatitis B virus surface antigen-containing solution, and eluting the absorbed hepatitis B virus surface antigen with a buffer solution of an ionic strength of 0.1 to 5.0 which is larger than that of the buffer solution for the equilibration dnd a pH range of 4 to 10, said sulfuric acid ester being prepared by treating a gel of crosslinked polysaccharide or cellulose with a sulfating agent in an organic solvent.
Claim 9
Independent Claim
128 words
A method for the purification of hepatitis B virus surface antigen, which comprises packing a gel of a sulfuric acid ester of a crosslinked polysaccharide or cellulose within a column, equilibrating the column with a buffer solution of an ionic strength of 0.001 to 1.0 and a pH range of 4 to 10, passing through the column a hepatitis B virus surface antigen-containing solution, and eluting the absorbed hepatitis B virus surface antigen with a buffer solution of an ionic strength of 0.1 to 5.0 which is larger than that of the buffer solution for the equilibration dnd a pH range of 4 to 10, said sulfuric acid ester being prepared by treating a gel of crosslinked polysaccharide or cellulose with a sulfating agent in an organic solvent.
Claim 10
Dependent Claim
25 words
The method according to claim 1, wherein the sulfating agent is a member selected from the group consisting of chlorosulfonic acid and anhydrous sulfuric acid.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-05-06 | 1988-11-05 | 1989-05-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-05-06 | 1992-11-05 | 1993-05-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-05-06 | 1996-11-05 | 1997-05-07 | $7700.00 | Window Closed / Lapsed |