US4520112A
Expired / Lapsed
US
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Assay method for organic calcium antagonist drugs and a kit for such an assay
Assignee: Johns Hopkins University
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Filing: 1983-03-09
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Granted: 1985-05-28
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Expires: 2003-03-09
Abstract
Abstract A method for measuring the level of organic calcium antagonist drug in a body fluid comprises preparing a mixture of a radioactive calcium antagonist drug, a body fluid containing a calcium antagonist drug and a calcium antagonist receptor material, measuring the radioactivity of the radioactive calcium antagonist drug bound to said calcium antagonist receptor material and deriving the concentration of the calcium antagonist drug in the body fluid from a standard curve indicating the concentration of calcium antagonist drug versus inhibition of binding of said radioactive calcium antagonist drug to said receptor sites caused by the calcium antagonist drug in said body fluid. A kit for measuring the level of an organic calcium drug comprises a receptacle containing a radioactive calcium antagonist drug, a calcium antagonist receptor material and a standard amount of a nonradioactive calcium antagonist drug.
Inventors
- 👤 Solomon H. Snyder
- 👤 Robert J. Gould
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 21:35
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; G01N33/9453; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/808; Y10S436/815
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
135 words
A method for measuring the level of an organic calcium antagonist drug including a pharmacologically active metabolite thereof in a body fluid comprising (a) preparing a mixture of a radioactive calcium antagonist drug, a body fluid containing a calcium antagonist drug and a calcium antagonist receptor material having receptor sites whereby said calcium antagonist drug competes with the binding of said radioactive calcium antagonist drug to said receptor sites, (b) measuring the radioactivity of the radioactive calcium antagonist drug bound to said calcium antagonist receptor material, and (c) deriving the concentration of the calcium antagonist drug in said body fluid from a standard curve indicating the concentration of calcium antagonist drug versus inhibition of binding of said radioactive calcium antagonist drug to said receptor sites caused by the calcium antagonist drug in said body fluid.
Claim 2
Independent Claim
135 words
A method for measuring the level of an organic calcium antagonist drug including a pharmacologically active metabolite thereof in a body fluid comprising (a) preparing a mixture of a radioactive calcium antagonist drug, a body fluid containing a calcium antagonist drug and a calcium antagonist receptor material having receptor sites whereby said calcium antagonist drug competes with the binding of said radioactive calcium antagonist drug to said receptor sites, (b) measuring the radioactivity of the radioactive calcium antagonist drug bound to said calcium antagonist receptor material, and (c) deriving the concentration of the calcium antagonist drug in said body fluid from a standard curve indicating the concentration of calcium antagonist drug versus inhibition of binding of said radioactive calcium antagonist drug to said receptor sites caused by the calcium antagonist drug in said body fluid.
Claim 3
Dependent Claim
35 words
The method of claim 1 wherein prior to measuring the radioactivity in step (b) the mixture resulting from step (a) is treated to remove therefrom any unbound radioactive calcium antagonist drug and calcium antagonist drug.
Claim 4
Dependent Claim
39 words
The method of claim 1 where in step (a) said mixture is incubated at a temperature and for a time sufficient to permit binding of said radioactive calcium antagonist drug and said calcium antagonist drug to said receptor sites.
Claim 5
Dependent Claim
23 words
The method of claim 3 wherein said mixture is incubated at ambient temperature for a period ranging from 2 minutes to 4 hours.
Claim 6
Dependent Claim
21 words
The method of claim 1 wherein the mixture in step (a) is buffered to a pH ranging from 6 to 9.
Claim 7
Dependent Claim
32 words
The method of claim 1 wherein said body fluid is present in the mixture of step (a) in an amount not greater than 10 percent of the total volume of said mixture.
Claim 8
Dependent Claim
32 words
The method of claim 1 wherein said body fluid is present in the mixture of step (a) in an amount not greater than 1 percent of the total volume of said mixture.
Claim 9
Dependent Claim
16 words
The method of claim 1 wherein said calcium antagonist receptor material is obtained from animal tissue.
Claim 10
Dependent Claim
14 words
The method of claim 8 wherein said tissue is brain, heart or skeletal muscle.
Claim 11
Dependent Claim
11 words
The method of claim 8 wherein said tissue is human tissue.
Claim 12
Dependent Claim
13 words
The method of claim 8 wherein the tissue is bovine or rodent tissue.
Claim 13
Dependent Claim
17 words
The method of claim 1 wherein the radioactive calcium antagonist drug is 3 H-nitrendipine or 3 H-nimodipine.
Claim 14
Dependent Claim
16 words
The method of claim 1 wherein said body fluid is urine, blood plasma or blood serum.
Claim 15
Dependent Claim
21 words
The method of claim 13 wherein the body fluid is purified so as to concentrate the calcium antagonist drug contained therein.
Claim 16
Dependent Claim
18 words
The method of claim 1 wherein said calcium antagonist drug is nifedipine, nimodipine, nitrendipine, verapamil, prenylamine or diltiazem.
Claim 17
Dependent Claim
54 words
A kit for measuring the level of an organic calcium antagonist drug, including a pharmacologically active metabolite thereof, in a body fluid, said kit comprising at least one receptacle containing one or more of a radioactive calcium antagonist drug, a calcium antagonist receptor material and a standard amount of a nonradioactive calcium antagonist drug.
Claim 18
Dependent Claim
54 words
A kit for measuring the level of an organic calcium antagonist drug, including a pharmacologically active metabolite thereof, in a body fluid, said kit comprising at least one receptacle containing one or more of a radioactive calcium antagonist drug, a calcium antagonist receptor material and a standard amount of a nonradioactive calcium antagonist drug.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-05-27 | 1988-11-26 | 1989-05-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-05-27 | 1992-11-26 | 1993-05-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-05-27 | 1996-11-26 | 1997-05-28 | $7700.00 | Window Closed / Lapsed |