US4526871A
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Conjugate obtained by coupling a lectin and a specific ligand, containing such a conjugate and its applications in biology
Assignee: Institut Pasteur
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Filing: 1981-05-20
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Granted: 1985-07-02
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Expires: 2001-05-20
Abstract
Abstract The present invention relates to a conjugate of a specific ligand and a lectin, by covalent bonding. The product of the invention may, for example, be the conjugate of Concanavalin A and an antigen, a hapten, an antibody, a hormone or its receptor, an enzyme or its inhibitor or a lectin. Coupling being effected by means of glutaraldehyde or p-benzoquinone.
Inventors
- 👤 Stratis Avrameas
- 👤 Jean-Luc Guesdon
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 20:27
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/543; G01N33/54306; G01N33/58; G01N33/585; G01N33/586; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/804; Y10S436/827
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
129 words
A process for the immunological assay of a biological substance, which comprises the steps of: (1) immobilizing a substance having a binding affinity for said biological substance to be determined; (2) incubating said substance with a medium containing said biological substance to be determined; (3) incubating, after washing, the resulting reaction medium with a conjugate of a lectin-specific ligand in solution in an aqueous medium containing an excess of a sugar specific to lectin, said ligand being capable of reacting specifically with said substance having an affinity to the biological substance to be determined or with said biological substance itself; (4) washing the resulting reaction medium and incubating it with a labeller bearing glycosidic fractions capable of reacting with said lectin; and (5) revealing said labeller by suitable means.
Claim 2
Dependent Claim
129 words
A process for the immunological assay of a biological substance, which comprises the steps of: (1) immobilizing a substance having a binding affinity for said biological substance to be determined; (2) incubating said substance with a medium containing said biological substance to be determined; (3) incubating, after washing, the resulting reaction medium with a conjugate of a lectin-specific ligand in solution in an aqueous medium containing an excess of a sugar specific to lectin, said ligand being capable of reacting specifically with said substance having an affinity to the biological substance to be determined or with said biological substance itself; (4) washing the resulting reaction medium and incubating it with a labeller bearing glycosidic fractions capable of reacting with said lectin; and (5) revealing said labeller by suitable means.
Claim 3
Dependent Claim
35 words
Process according to claim 1, wherein said labeller is chosen from the group consisting of an enzyme, a substance containing a radioactive isotope, a fluorochrome, and a particulate substance capable of reacting with said lectin.
Claim 4
Dependent Claim
14 words
Process according to claim 2, wherein the particulate substance consists of red blood cells.
Claim 5
Dependent Claim
49 words
Process according to claim 3, wherein in order to reveal the red blood cells used as labeller the unreacted red blood cells used as labellers are removed, the red blood cells bound to the lectin-ligand conjugate are lysed, and the substances released by the red blood cells are determined.
Claim 6
Dependent Claim
13 words
Process according to claim 4, wherein the lysis is effected with distilled water.
Claim 7
Dependent Claim
32 words
Process according to claim 1 or 2, wherein the liquid medium containing the biological substance to be assayed is a serum and wherein the serum is previously absorbed by red blood cells.
Claim 8
Dependent Claim
95 words
Process according to claim 6, wherein said serum is absorbed by red blood cells according to the procedure which comprises the following steps: (1) decomplementing of the serum by heating at about 56° C. for about 30 minutes; (2) adding an excess of red blood cells of sheep to said serum, in order to obtain a concentration in red blood cells of 10%; (3) leaving said serum at ambient temperature for about one hour under smooth stirring; and (4) centrifuging in order to recover the purified serum and above the bottom of red blood cells.
Claim 9
Dependent Claim
22 words
Process according to claim 6, wherein the serum is absorbed by red blood cells treated with a tanning agent, such as glutaraldehyde.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-07-01 | 1988-12-31 | 1989-07-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-07-01 | 1992-12-31 | 1993-07-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-07-01 | 1996-12-31 | 1997-07-02 | $7700.00 | Window Closed / Lapsed |