US4543326A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Stabilization of oxidase
Assignee: Fujifilm Wako Pure Chemical Corp
•
Filing: 1982-11-12
•
Granted: 1985-09-24
•
Expires: 2002-11-12
Abstract
Abstract An oxidase selected from the group consisting of glycerol-3-phosphate oxidase, choline oxidase and glucose oxidase can be stabilized by adding thereto an acidic amino acid or a salt thereof. The resulting stabilized composition can be used for quantitatively determining the content of glycerol-3-phosphate, choline, triglyceride, glucose, etc., in a biological fluid.
Inventors
- 👤 Yoshinobu Miyashita
- 👤 Shinji Satomura
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 09:10
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0006; C12N9/96; C12Q; C12Q1/00; C12Q1/26; Y; Y10; Y10S; Y10S435/00; Y10S435/81
Claims Analysis
Total Claims: 34
Claim 1
Independent Claim
44 words
A process for stabilizing an oxidase which comprises adding an acidic amino acid or a salt thereof in an amount of 1 to 5% by weight to an aqueous solution of an oxidase selected from the group consisting of glycerol-3-phosphate oxidase, and choline oxidase.
Claim 2
Dependent Claim
44 words
A process for stabilizing an oxidase which comprises adding an acidic amino acid or a salt thereof in an amount of 1 to 5% by weight to an aqueous solution of an oxidase selected from the group consisting of glycerol-3-phosphate oxidase, and choline oxidase.
Claim 3
Dependent Claim
12 words
A process according to claim 1, wherein the oxidase is glycerol-3-phosphate oxidase.
Claim 4
Dependent Claim
12 words
A process according to claim 1, wherein the oxidase is choline oxidase.
Claim 5
Dependent Claim
24 words
A process according to claim 1, wherein the acidic amino acid or a salt thereof is a buffer solution-soluble salt of acidic amino acid.
Claim 6
Dependent Claim
49 words
A process according to claim 1, wherein the acidic amino acid or a salt thereof is represented by the formula: ##STR6## wherein A is a lower alkylene group having 1 to 5 carbon atoms; and Y and Y' are independently hydrogen, a NH 4 group or an alkali metal.
Claim 7
Dependent Claim
18 words
A process according to claim 1, wherein the acidic amino acid or a salt thereof is sodium glutamate.
Claim 8
Dependent Claim
18 words
A process according to claim 1, wherein the acidic amino acid or a salt thereof is sodium aspartate.
Claim 9
Dependent Claim
96 words
A process for quantitatively determining the glycerol-3-phosphate content in a biological fluid which comprises adding to the biological fluid an aqueous solution containing glycerol-3-phosphate oxidase in an amount of 1 to 20 units/ml, a buffer solution in an amount sufficient to maintain the pH near neutral value, an acidic amino acid or a salt thereof in an amount of 1 to 5% by weight based on the weight of the aqueous solution containing the oxidase, peroxidase, 4-aminoantipyrine, and an oxidizable color producing indicator in amounts sufficient to produce a color, and measuring the color produced colorimetrically.
Claim 10
Dependent Claim
96 words
A process for quantitatively determining the glycerol-3-phosphate content in a biological fluid which comprises adding to the biological fluid an aqueous solution containing glycerol-3-phosphate oxidase in an amount of 1 to 20 units/ml, a buffer solution in an amount sufficient to maintain the pH near neutral value, an acidic amino acid or a salt thereof in an amount of 1 to 5% by weight based on the weight of the aqueous solution containing the oxidase, peroxidase, 4-aminoantipyrine, and an oxidizable color producing indicator in amounts sufficient to produce a color, and measuring the color produced colorimetrically.
Claim 11
Dependent Claim
24 words
A process according to claim 8, wherein the acidic amino acid or a salt thereof is an alkali metal salt of acidic amino acid.
Claim 12
Dependent Claim
21 words
A process according to claim 9, wherein the alkali metal salt of acidic amino acid is sodium aspartate or sodium glutamate.
Claim 13
Dependent Claim
92 words
A process for quantitatively determining the triglyceride content in a biological fluid which comprises adding to the biological fluid an aqueous solution containing glycerol-3-phosphate oxidase in an amount of 1 to 20 units/ml, a buffer solution in an amount sufficient to maintain the pH near neutral value, an acidic amino acid or a salt thereof in an amount of 1 to 5% by weight of the aqueous solution, lipase, glycerol kinase, peroxidase, 4-aminoantipyrine and an oxidizable color producing indicator in amounts sufficient to produce a color, and measuring the color produced colorimetrically.
Claim 14
Dependent Claim
92 words
A process for quantitatively determining the triglyceride content in a biological fluid which comprises adding to the biological fluid an aqueous solution containing glycerol-3-phosphate oxidase in an amount of 1 to 20 units/ml, a buffer solution in an amount sufficient to maintain the pH near neutral value, an acidic amino acid or a salt thereof in an amount of 1 to 5% by weight of the aqueous solution, lipase, glycerol kinase, peroxidase, 4-aminoantipyrine and an oxidizable color producing indicator in amounts sufficient to produce a color, and measuring the color produced colorimetrically.
Claim 15
Dependent Claim
24 words
A process according to claim 11, wherein the acidic amino acid or a salt thereof is an alkali metal salt of acidic amino acid.
Claim 16
Dependent Claim
21 words
A process according to claim 12, wherein the alkali metal salt of acidic amino acid is sodium aspartate or sodium glutamate.
Claim 17
Dependent Claim
93 words
A process for quantitatively determining the choline content in a biological fluid which comprises adding to the biological fluid an aqueous solution containing choline oxidase in an amount of 1 to 20 units/ml, a buffer solution in an amount sufficient to maintain the pH near neutral value, an acidic amino acid or a salt thereof in an amount of 1 to 5% by weight, based upon the weight of the aqueous solution, peroxidase, 4-aminoantipyrine and an oxidizable color producing indicator in sufficient amounts to produce a color, and measuring the color produced colormetrically.
Claim 18
Dependent Claim
93 words
A process for quantitatively determining the choline content in a biological fluid which comprises adding to the biological fluid an aqueous solution containing choline oxidase in an amount of 1 to 20 units/ml, a buffer solution in an amount sufficient to maintain the pH near neutral value, an acidic amino acid or a salt thereof in an amount of 1 to 5% by weight, based upon the weight of the aqueous solution, peroxidase, 4-aminoantipyrine and an oxidizable color producing indicator in sufficient amounts to produce a color, and measuring the color produced colormetrically.
Claim 19
Dependent Claim
24 words
A process according to claim 14, wherein the acidic amino acid or a salt thereof is an alkali metal salt of acidic amino acid.
Claim 20
Dependent Claim
21 words
A process according to claim 15, wherein the alkali metal salt of acidic amino acid is sodium aspartate or sodium glutamate.
Claim 21
Dependent Claim
86 words
A process for measuring the activity of choline esterase in a biological fluid which comprises adding to the biological fluid an aqueous solution containing choline oxidase in an amount of 1 to 20 units/ml, a buffer solution in an amount of 1 to 5% by weight based on the weight of the solution, peroxidase, choline benzoyl chloride, 4-aminoantipyrine and an oxidizable color producing indicator in amounts sufficient to produce a color, adding to the resulting mixture a reaction stopper solution, and measuring the color produced colorimetrically.
Claim 22
Dependent Claim
86 words
A process for measuring the activity of choline esterase in a biological fluid which comprises adding to the biological fluid an aqueous solution containing choline oxidase in an amount of 1 to 20 units/ml, a buffer solution in an amount of 1 to 5% by weight based on the weight of the solution, peroxidase, choline benzoyl chloride, 4-aminoantipyrine and an oxidizable color producing indicator in amounts sufficient to produce a color, adding to the resulting mixture a reaction stopper solution, and measuring the color produced colorimetrically.
Claim 23
Dependent Claim
24 words
A process according to claim 17, wherein the acidic amino acid or a salt thereof is an alkali metal salt of acidic amino acid.
Claim 24
Dependent Claim
21 words
A process according to claim 18, wherein the alkali metal salt of acidic amino acid is sodium aspartate or sodium glutamate.
Claim 25
Dependent Claim
39 words
A process according to any of claims 1, 8, 11, 14, or 17 in which the acidic amino acid is selected from the group consisting of glutamic acid, aspartic acid, alpha-aminoadipic acid and salts of these acidic amino acids.
Claim 26
Dependent Claim
53 words
A stabilized composition comprising an oxidase selected from the group consisting of glycerol-3-phosphate oxidase, and choline oxidase, an acidic amino acid or a salt thereof in an amount equal to 1 to 5% by weight of the composition, and a buffer solution in an amount sufficient to maintain the pH near neutral value.
Claim 27
Dependent Claim
53 words
A stabilized composition comprising an oxidase selected from the group consisting of glycerol-3-phosphate oxidase, and choline oxidase, an acidic amino acid or a salt thereof in an amount equal to 1 to 5% by weight of the composition, and a buffer solution in an amount sufficient to maintain the pH near neutral value.
Claim 28
Dependent Claim
12 words
A composition according to claim 21, wherein the oxidase is glycerol-3-phosphate oxidase.
Claim 29
Dependent Claim
12 words
A composition according to claim 21, wherein the oxidase is choline oxidase.
Claim 30
Dependent Claim
50 words
A stabilized composition according to claim 21, wherein the acidic amino acid or a salt thereof is represented by the formula: ##STR7## wherein A is a lower alkylene group having 1 to 5 carbon atoms; and Y and Y' are independently hydrogen, a NH 4 group or an alkali metal.
Claim 31
Dependent Claim
13 words
A composition according to claim 21, which further comprises a color producing reagent.
Claim 32
Dependent Claim
18 words
A composition according to claim 21, wherein the acidic amino acid or a salt thereof is sodium glutamate.
Claim 33
Dependent Claim
18 words
A composition according to claim 21, wherein the acidic amino acid or a salt thereof is sodium aspartate.
Claim 34
Dependent Claim
32 words
A composition according to claim 21 in which the acidic amino acid is selected from the group consisting of glutamic acid, aspartic acid, alpha-aminoadipic acid and salts of these acidic amino acids.
References Cited (Backward Citations)
US3096251A
Cited
US3539450A
Cited
US3964974A
Cited
US4135980A
Cited
US4245050A
Cited
US4241178A
Cited
US4275161A
Cited
JPS5534001A
Cited
US4288541A
Cited
US4279993A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-09-23 | 1989-03-25 | 1989-09-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-09-23 | 1993-03-25 | 1993-09-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-09-23 | 1997-03-25 | 1997-09-24 | $7700.00 | Window Closed / Lapsed |