US4546083A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and device for cell culture growth
Assignee: Stolle Research and Development Corp
•
Filing: 1983-04-22
•
Granted: 1985-10-08
•
Expires: 2003-04-22
Abstract
Abstract A cell culture device is disclosed for the cultivation of animal, plant, microbiological or artificial cells. The device involves a three-dimensional arrangement of fibers within a housing arranged to provide maximum exposed fiber surface flow channel diameter while also reducing the tortuosity of the flow path. Cells are bound to the fibers to allow them to contact nutrient fluid solution and thereby to remove any substances originating in said cells, such as viruses and pharmaceuticals.
Inventors
- 👤 William E. Meyers
- 👤 Lee R. Beck
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 12:58
CPC Classifications
C; C12; C12M; C12M23/00; C12M23/02; C12M23/04; C12M23/06; C12M25/00; C12M25/10; C12M29/00
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
217 words
A method of cultivating cells in vitro which comprises: culturing cells selected from the group consisting of cells of animal, vegetable, microbiological and artificial origin in a cell culture device comprising an elongated housing of impermeable material, closed at its ends by means of impermeable end plates, said housing on its interior having a plurality of axial ribs extending substantially throughout the length thereof, an inlet port in one of said end plates, an outlet port in the other end of said end plates, said ribs continuing radially in said other of said end plates, an impermeable spindle axially disposed in said housing and having axially disposed grooves in its periphery, a cylindrical port secured to one end of said spindle and in communication with said inlet port and with said grooves, said spindle at its other end terminating in a conical tip axially disposed with respect to said outlet port with an annular space therebetween, and a spool of fiber helically wound on said spindle to substantially fill the interior of said housing into said ribs, whereby fluid entering said inlet port flows along said spindle grooves, passes through said fibrous spool, flows alongside said housing between said ribs, and then between said conical tip and said outlet port, said fiber having attached thereon the cells.
Claim 2
Independent Claim
217 words
A method of cultivating cells in vitro which comprises: culturing cells selected from the group consisting of cells of animal, vegetable, microbiological and artificial origin in a cell culture device comprising an elongated housing of impermeable material, closed at its ends by means of impermeable end plates, said housing on its interior having a plurality of axial ribs extending substantially throughout the length thereof, an inlet port in one of said end plates, an outlet port in the other end of said end plates, said ribs continuing radially in said other of said end plates, an impermeable spindle axially disposed in said housing and having axially disposed grooves in its periphery, a cylindrical port secured to one end of said spindle and in communication with said inlet port and with said grooves, said spindle at its other end terminating in a conical tip axially disposed with respect to said outlet port with an annular space therebetween, and a spool of fiber helically wound on said spindle to substantially fill the interior of said housing into said ribs, whereby fluid entering said inlet port flows along said spindle grooves, passes through said fibrous spool, flows alongside said housing between said ribs, and then between said conical tip and said outlet port, said fiber having attached thereon the cells.
Claim 3
Dependent Claim
252 words
In a method of obtaining a substance which originates in cells of animal, plant, microbiological or artifical origin, comprising: (a) cultivating cells selected from the group consisting of cells of animal, vegetable, microbiological or artificial origin in vitro while maintaining said cells immobilized, and (b) thereafter collecting said products from said cells; wherein the improvement comprises said cells are immobilized in a cell culturing device comprising an elongated housing of impermeable material, closed at its ends by means of impermeable end plates, said housing on its interior having a plurality of axial ribs extending substantially throughout the length thereof, an inlet port in one of said end plates, an outlet port in the other end of said end plates, said ribs continuing radially in said other of said end plates, an impermeable spindle axially disposed in said housing and having axially disposed grooves in its periphery, a cylindrical port secured to one end of said spindle and in communication with said inlet port and with said grooves, said spindle at its other end terminating in a conical tip axially disposed with respect to said outlet port with an annular space therebetween, and a spool of fiber helically wound on said spindle to substantially fill the interior of said housing into said ribs, whereby fluid entering said inlet port flows along said spindle grooves, passes through said fibrous spool, flows alongside said housing between said ribs, and then between said conical tip and said outlet port, said fiber having attached thereon the cells.
Claim 4
Dependent Claim
252 words
In a method of obtaining a substance which originates in cells of animal, plant, microbiological or artifical origin, comprising: (a) cultivating cells selected from the group consisting of cells of animal, vegetable, microbiological or artificial origin in vitro while maintaining said cells immobilized, and (b) thereafter collecting said products from said cells; wherein the improvement comprises said cells are immobilized in a cell culturing device comprising an elongated housing of impermeable material, closed at its ends by means of impermeable end plates, said housing on its interior having a plurality of axial ribs extending substantially throughout the length thereof, an inlet port in one of said end plates, an outlet port in the other end of said end plates, said ribs continuing radially in said other of said end plates, an impermeable spindle axially disposed in said housing and having axially disposed grooves in its periphery, a cylindrical port secured to one end of said spindle and in communication with said inlet port and with said grooves, said spindle at its other end terminating in a conical tip axially disposed with respect to said outlet port with an annular space therebetween, and a spool of fiber helically wound on said spindle to substantially fill the interior of said housing into said ribs, whereby fluid entering said inlet port flows along said spindle grooves, passes through said fibrous spool, flows alongside said housing between said ribs, and then between said conical tip and said outlet port, said fiber having attached thereon the cells.
Claim 5
Dependent Claim
12 words
The method of claim 1 wherein the cells are virus producing cells.
Claim 6
Dependent Claim
12 words
The method of claim 1 wherein the cells are of animal origin.
Claim 7
Dependent Claim
12 words
The method of claim 4 wherein the cells are of mammalian origin.
Claim 8
Dependent Claim
10 words
The method of claim 1 wherein the cells are hybridomas.
Claim 9
Dependent Claim
14 words
The method of claim 1 wherein the cells are directly immobilized on the fibers.
Claim 10
Dependent Claim
28 words
The method of claim 1 wherein the cells are immobilized on said fibers by means of chemical anchoring compounds selected from the group consisting of polylysine and collagen.
Claim 11
Dependent Claim
13 words
The method of claim 1 wherein the fiber is an electrically charged fiber.
Claim 12
Dependent Claim
17 words
The method of claim 9 wherein the fiber was electrically charged by glow discharge or corona discharge.
Claim 13
Dependent Claim
26 words
The method of claim 1 wherein the fiber was charged with a gas selected from the group consisting of O 2 , NH 4 and argon.
Claim 14
Dependent Claim
12 words
The method of claim 2 wherein the cells are virus producing cells.
Claim 15
Dependent Claim
12 words
The method of claim 2 wherein the cells are of animal origin.
Claim 16
Dependent Claim
12 words
The method of claim 13 wherein the cells are of mammalian origin.
Claim 17
Dependent Claim
10 words
The method of claim 2 wherein the cells are hybridomas.
Claim 18
Dependent Claim
14 words
The method of claim 2 wherein the cells are directly immobilized on said fibers.
Claim 19
Dependent Claim
28 words
The method of claim 2 wherein the cells are immobilized on the fibers by means of chemical anchoring compounds selected from the group consisting of polylysine and collagen.
Claim 20
Dependent Claim
13 words
The method of claim 2 wherein the fiber is an electrically charged fiber.
Claim 21
Dependent Claim
17 words
The method of claim 18 wherein the fiber was electrically charged by glow discharge or corona discharge.
Claim 22
Dependent Claim
26 words
The method of claim 2 wherein the fiber was charged with a gas selected from the group consisting of O 2 , NH 4 and argon.
References Cited (Backward Citations)
US3407120A
Cited
US3734851A
Cited
US3997396A
Cited
US4087327A
Cited
US4332904A
Cited
US4220725A
Cited
US4252653A
Cited
US4283495A
Cited
US4283495B1
Cited
US4442206A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-10-07 | 1989-04-08 | 1989-10-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-10-07 | 1993-04-08 | 1993-10-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-10-07 | 1997-04-08 | 1997-10-08 | $7700.00 | Window Closed / Lapsed |