US4559303A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Carrier composed of particulate polymer
Assignee: JSR Corp
•
Filing: 1983-05-20
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Granted: 1985-12-17
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Expires: 2003-05-20
Abstract
Abstract A carrier composed of particulate polymer of at least one monomer represented by the general formula: ##STR1## wherein R 1 and R 2 , which may be the same or different, are hydrogen atoms or alkyl groups having 1 to 4 carbon atoms; R 3 is a straight-chain or branched alkylene group having 2 or 3 carbon atoms, which group may be substituted by a hydroxyl group; X's, which may be the same or different, are halogen atoms; m is zero or an integer of 1 to 10; and n is an integer of 1 to 5, or a particulate polymer of said monomer and at least one other copolymerizable monomer. Said carrier is suitable for supporting a biological substance such as an immunoreactive substance, an enzyme, a cell, a cell-discriminating substance, and the like, particularly an immunoreactive substance.
Inventors
- 👤 Seiji Aotani
- 👤 Hisanori Kanayama
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 03:40
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; C; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/087; Y; Y10; Y10S; Y10S436/00; Y10S436/823; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
137 words
A carrier having a biological substance supported thereon, wherein said carrier consists essentially of polymer particles produced by Polymerizing a monomer having the formula: ##STR3## wherein R 1 and R 2 , which are the same or different, are hydrogen atoms or alkyl groups having 1 to 4 carbon atoms; R 3 is a straight-chain or branched alkylene group having 2 or 3 carbon atoms, which is unsubstituted or substituted by a hydroxyl group; X is one or more of the same or different halogen atoms; m is zero or an integer of 1 to 10; and n is an integer of 1 to 5, or by polymerizing said monomer with at least one other vinyl monomer copolymerizable therewith; and wherein said biological substance is selected from the group of immunoreactive substances, enzymes, cells or cell-discriminating substances.
Claim 2
Dependent Claim
137 words
A carrier having a biological substance supported thereon, wherein said carrier consists essentially of polymer particles produced by Polymerizing a monomer having the formula: ##STR3## wherein R 1 and R 2 , which are the same or different, are hydrogen atoms or alkyl groups having 1 to 4 carbon atoms; R 3 is a straight-chain or branched alkylene group having 2 or 3 carbon atoms, which is unsubstituted or substituted by a hydroxyl group; X is one or more of the same or different halogen atoms; m is zero or an integer of 1 to 10; and n is an integer of 1 to 5, or by polymerizing said monomer with at least one other vinyl monomer copolymerizable therewith; and wherein said biological substance is selected from the group of immunoreactive substances, enzymes, cells or cell-discriminating substances.
Claim 3
Dependent Claim
14 words
The carrier according to claim 1, which has a particle diameter of 0.1-10 μm.
Claim 4
Dependent Claim
16 words
The carrier according to claim 1, which has a particle diameter of 0.3 to 1 μm.
Claim 5
Dependent Claim
18 words
The carrier according to claim 2, which has a specific gravity of 1.2 to 2 g/cm 3 .
Claim 6
Dependent Claim
18 words
The carrier according to claim 3, which has a specific gravity of 1.3 to 1.8 g/cm 3 .
Claim 7
Dependent Claim
47 words
A carrier according to claim 1, wherein the said other vinyl monomer is selected from the group consisting of aromatic alkenyl compounds, α,β-unsaturated carboxylic acid, esters of α,β-unsaturated carboxylic acid, α,β-unsaturated carboxylic amides, α,β-unsaturated nitrile compounds, vinyl halides, conjugated diene compounds, and vinyl esters of fatty acids.
Claim 8
Dependent Claim
30 words
The carrier according to claim 1, wherein the amount of the monomer represented by the formula (I) is at least 10 mole % of the total amount of, the monomers.
Claim 9
Dependent Claim
30 words
The carrier according to claim 1, wherein the amount of the monomer represented by the formula (I) is 15 to 60 mole % of the total amount of the monomers.
Claim 10
Dependent Claim
38 words
The carrier according to claim 1, wherein the monomer represented by the formula (I) is monofluorophenyl (meth)acrylate, trichlorophenyl (meth)acrylate, pentachlorophenyl (meth)acrylate, monobromophenyl (meth)acrylate, tribromophenyl meth)acrylate, pentabromophenyl (meth)acrylate, monoiodophenyl (meth)acrylate, 2-(tri-bromophenoxy)ethyl (meth)acrylate, 2-(tribromophenoxyethoxy)ethyl (meth)acrylate, 2-(pentachlorophenoxy)propyl (meth)acrylate or 1-(tribromophenoxy)2-hydroxypropyl (meth)acrylate.
Claim 11
Dependent Claim
27 words
The carrier according to claim 1, wherein the polymer particles are those obtained by a soap-free polymerization method, a solution-precipitation polymerization method or a seed polymerization method.
Claim 12
Dependent Claim
130 words
A reagent for detecting an antigen or antibody consisting of a carrier consisting essentially of polymer particles obtained by polymerizing a monomer having by the formula: ##STR4## wherein R 1 and R 2 , which are the same or different, are hydrogen atoms or alkyl groups having 1 to 4 carbon atoms; R 3 is a straight-chain or branched alkylene group having 2 or 3 carbon atoms, which is unsubstituted or substituted by a hydroxyl group; X is one or more of the same or different halogen atoms; m is zero or an integer of 1 to 10; and n is an integer of 1 to 5; or by polymerizing said monomer with at least one other vinyl monomer copolymerizable therewith; and wherein said biological substance is an immunoreactive substance.
Claim 13
Dependent Claim
130 words
A reagent for detecting an antigen or antibody consisting of a carrier consisting essentially of polymer particles obtained by polymerizing a monomer having by the formula: ##STR4## wherein R 1 and R 2 , which are the same or different, are hydrogen atoms or alkyl groups having 1 to 4 carbon atoms; R 3 is a straight-chain or branched alkylene group having 2 or 3 carbon atoms, which is unsubstituted or substituted by a hydroxyl group; X is one or more of the same or different halogen atoms; m is zero or an integer of 1 to 10; and n is an integer of 1 to 5; or by polymerizing said monomer with at least one other vinyl monomer copolymerizable therewith; and wherein said biological substance is an immunoreactive substance.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-12-16 | 1989-06-17 | 1989-12-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-12-16 | 1993-06-17 | 1993-12-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-12-16 | 1997-06-17 | 1997-12-17 | $7700.00 | Window Closed / Lapsed |