Serum-free cell culture medium and process for making same

Claim 2

A substitute for serum in cell culture media capable of long-term culture of suspension and monolayer cells and hybridoma cells and capable of supporting fusion of cells to form hybridomas, comprising fetuin, transferrin, albumin, cholesterol and phosphatidylcholine having the general formula: ##STR3## wherein R 1 is a saturated fatty acid residue derived from the group consisting of lauric acid, myristic acid, palmitic acid and stearic acid; and, R 2 is an unsaturated fatty acid residue derived from the group consisting of oleic acid, linoleic acid, linolenic acid, and arachidonic acid; or R 1 is an unsaturated fatty acid residue derived from the group consisting of oleic acid, linoleic acid, linolenic acid, and arachidonic acid; and, R 2 is a saturated fatty acid residue derived from the group consisting of lauric acid, myristic acid, palmitic acid and stearic acid; and, R 1 and R 2 are chemically bonded to the respective carbon atoms of the phosphatidylcholine.

Claim 3

The serum substitute according to claim 1, wherein the transferrin is partially to fully iron saturated by addition of up to 1.89 milligrams per liter of ferric chloride.

Claim 4

The serum substitute according to claim 1, wherein R 1 is derived from oleic acid and R 2 is derived from palmitic acid.

Claim 5

The serum substitute of claim 2, wherein the concentration of transferrin is in the range of about 3 to 300 milligrams per liter of medium, the concentration of fetuin is in the range of about 9 micrograms to 1500 milligrams per liter of medium and the concentration of defined phosphatidylcholine is in the range of about 180 micrograms to 18 milligrams per liter of medium.

Claim 6

In cell culture media capable of long-term growth of suspension cells, monolayer cells and hybridoma cells, and capable of fostering the fusion of cells to form hybridomas, such media including salts, amino acids, carbohydrates, albumin, at least one steroid and unbound fatty acid, the improvement comprising the addition of fetuin, transferrin and phosphatidylcholine having the general formula: ##STR4## wherein: R 1 is an unsaturated fatty acid residue derived from the group consisting of oleic acid, linoleic acid, linolenic acid, and arachidonic acid; and, R 2 is a saturated fatty acid residue derived from the group consisting of lauric acid, myristic acid, palmitic acid and stearic acid; or, R 1 is a saturated fatty acid residue derived from the group consisting of lauric acid, myristic acid, palmitic acid, and stearic acid; and, R 2 is an unsaturated fatty acid residue derived from the group consisting of oleic acid, linoleic acid, and arachidonic acid; and, R 1 and R 2 are chemically bonded to their respective carbon atoms of the phosphatidylcholine.

Claim 7

In cell culture media capable of long-term growth of suspension cells, monolayer cells and hybridoma cells, and capable of fostering the fusion of cells to form hybridomas, such media including salts, amino acids, carbohydrates, albumin, at least one steroid and unbound fatty acid, the improvement comprising the addition of fetuin, transferrin and phosphatidylcholine having the general formula: ##STR4## wherein: R 1 is an unsaturated fatty acid residue derived from the group consisting of oleic acid, linoleic acid, linolenic acid, and arachidonic acid; and, R 2 is a saturated fatty acid residue derived from the group consisting of lauric acid, myristic acid, palmitic acid and stearic acid; or, R 1 is a saturated fatty acid residue derived from the group consisting of lauric acid, myristic acid, palmitic acid, and stearic acid; and, R 2 is an unsaturated fatty acid residue derived from the group consisting of oleic acid, linoleic acid, and arachidonic acid; and, R 1 and R 2 are chemically bonded to their respective carbon atoms of the phosphatidylcholine.

Claim 8

The cell culture media according to claim 5, wherein the transferrin is partially to fully iron saturated by addition of up to 1.89 milligrams per liter of ferric chloride.

Claim 9

The cell culture media according to claim 5, wherein R 1 is derived from oleic acid and R 2 is derived from palmitic acid.

Claim 10

The cell culture media according to claim 5 wherein the amino acids are selected from the group consisting of L-alanine, L-asparagine, L-arginine.HCL, L-aspartic acid, L-cystine, L-cystine 2HCl, L-glutamic acid, L-glutamine, glycine, L-histidine.HCl.H 2 O, L-isoleucine, L-leucine, L-lysine.HCl, L-methionine, L-phenylalanine, L-proline, L-serine, L-threonine, L-tryptophane, L-tyrosine (disodium salt) and L-valine.

Claim 11

The cell culture media according to claim 5, wherein the salts are selected from the group consisting of calcium chloride, ferric nitrate, potassium chloride, magnesium sulfate, sodium chloride, sodium bicarbonate, sodium phosphate (monobasic), sodium selenite and sodium pyruvate.

Claim 12

The cell culture media according to claim 5, wherein the carbohydrate includes D-glucose.

Claim 13

The cell culture media according to claim 5, further comprising vitamins and cofactors selected from the group consisting of biotin, D-calcium pantothenate, choline chloride, folic acid, I-inositol, nicotinamide, pyridoxal HCl, riboflavin, thiamine.HCl, and vitamin B 12 .

Claim 14

The cell culture media of claim 5, wherein the concentration of fetuin is in the range of about 9 micrograms to 1500 milligrams per liter of medium, the concentration of transferrin is in the range of about 3 to 300 milligrams per liter of medium and the concentration of defined phosphatidylcholine is in the range of about 180 micrograms to 18 milligrams per liter of medium.

Claim 15

In cell culture media capable of culturing suspension cells, monolayer cells and hybridoma cells are capable of supporting fusion of cells to form hybridomas, the media including Dulbecco's Modified Eagle Medium, the improvement further including in the cell culture media fetuin, partially to fully iron saturated transferrin, and phosphatidylcholine having the general formula: ##STR5## wherein: R 1 is an unsaturated fatty acid residue derived from the group consisting of oleic acid, linoleic acid, linolenic acid, or arachidonic acid; and, R 2 is a saturated fatty acid residue derived from the group consisting of lauric acid, myristic acid, palmitic acid or stearic acid; or conversely, R 1 is a saturated fatty acid residue derived from the group consisting of lauric acid, myristic acid, palmitic acid and stearic acid; and, R 2 is an unsaturated fatty acid residue derived from the group consisting of oleic acid, linoleic acid, linolenic acid, and arachidonic acid; and, R 1 and R 2 are chemically bonded to their respective carbon atoms of the phosphatidylcholine.

Claim 16

In cell culture media capable of culturing suspension cells, monolayer cells and hybridoma cells are capable of supporting fusion of cells to form hybridomas, the media including Dulbecco's Modified Eagle Medium, the improvement further including in the cell culture media fetuin, partially to fully iron saturated transferrin, and phosphatidylcholine having the general formula: ##STR5## wherein: R 1 is an unsaturated fatty acid residue derived from the group consisting of oleic acid, linoleic acid, linolenic acid, or arachidonic acid; and, R 2 is a saturated fatty acid residue derived from the group consisting of lauric acid, myristic acid, palmitic acid or stearic acid; or conversely, R 1 is a saturated fatty acid residue derived from the group consisting of lauric acid, myristic acid, palmitic acid and stearic acid; and, R 2 is an unsaturated fatty acid residue derived from the group consisting of oleic acid, linoleic acid, linolenic acid, and arachidonic acid; and, R 1 and R 2 are chemically bonded to their respective carbon atoms of the phosphatidylcholine.

Claim 17

The cell culture media according to claim 13, wherein R 1 is derived from oleic acid and R 2 is derived from palmitic acid.

Claim 18

The cell culture media according to claim 13, further comprising one or more amino acids selected from the group consisting of L-alanine, L-aspargine, L-aspartic acid, L-cystine, L-glutamic acid, and L-proline.

Claim 19

The cell culture media according to claim 13, further comprising additional amounts of D-glucose.

Claim 20

The cell culture media according to claim 13, further comprising additional amounts of organic and/or inorganic salts selected from the group consisting of sodium bicarbonate, sodium selenite, and sodium pyruvate.

Claim 21

The cell culture media according to claim 13, further comprising additional amounts of vitamins and cofactors selected from the group consisting of biotin and vitamin B 12 .

Claim 22

The cell culture media according to claim 13, wherein the concentration of fetuin is in the range of about 9 micrograms to 1500 milligrams per liter of medium, the concentration of transferrin is in the range of about 3 to 300 milligrams per liter of medium and the concentration of defined phosphatidylcholine is in the range of about 180 micrograms to 18 milligrams per liter of medium.

Claim 23

An improved process for culturing suspension, monolayer and hybridoma cells and fusing cells to form hybridoma cells comprising the step of growing the cells in a culture medium comprising salts, amino acids, carbohydrates, albumin, one or more steroids, unbound fatty acids, fetuin in the range of about 9 micrograms to 1500 milligrams per liter of medium, transferrin in the range of about 3 milligrams to 300 milligrams per liter of medium and a defined phosphatidylcholine selected from the group consisting of 1-palmitoyl-2-oleoyl phosphatidylcholine and 1-oleoyl-2-palmitoyl phosphatidylcholine, wherein such defined phosphatidylcholine is in the range of about 180 micrograms to 18 milligrams per liter of medium.

Claim 24

An improved process for culturing suspension, monolayer and hybridoma cells and fusing cells to form hybridoma cells comprising the step of growing the cells in a culture medium comprising salts, amino acids, carbohydrates, albumin, one or more steroids, unbound fatty acids, fetuin in the range of about 9 micrograms to 1500 milligrams per liter of medium, transferrin in the range of about 3 milligrams to 300 milligrams per liter of medium and a defined phosphatidylcholine selected from the group consisting of 1-palmitoyl-2-oleoyl phosphatidylcholine and 1-oleoyl-2-palmitoyl phosphatidylcholine, wherein such defined phosphatidylcholine is in the range of about 180 micrograms to 18 milligrams per liter of medium.