US4560704A
Expired / Lapsed
US
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Polyamine based bonded phase chromatography
Assignee: Purdue Research Foundation
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Filing: 1982-11-12
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Granted: 1985-12-24
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Expires: 2002-11-12
Abstract
Abstract Bonded phase chromatography is disclosed wherein an organosilane reacts with a polyamine to form a support matrix which is attached to the inorganic surface of a support structure. In forming an organic polyamine support matrix, a neutral organosilane is bonded to the inorganic surface with the organosilane having a functional group sufficiently reactive so that an organic amine polymer reacts with the functional group to cause covalent bonding of the organic amine polymer to the inorganic surface. By addition of an organic stationary phase group, the organic polyamine support matrix is formed into any predetermined one of a plurality of types of chromatography supports.
Inventors
- 👤 Frederick E. Regnier
- 👤 Shri P. Gupta
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 22:54
CPC Classifications
B; B01; B01J; B01J20/00; B01J20/281; B01J20/286; B01J20/289; B01J20/30; B01J20/32; B01J20/3214; B01J20/3217; B01J20/3219; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3246; B01J20/3257; B01J20/3263; B01J20/3268; B01J20/3272
Claims Analysis
Total Claims: 37
Claim 1
Independent Claim
85 words
A process for producing a bonded support for a chromatographic system, said process comprising: providing a support structure having an inorganic surface; providing a preformed polyamine; bonding a neutral organosilane to said inorganic surface with said neutral organosilane having a functional group sufficiently reactive so that said preformed polyamine reacts with said functional group to cause covalent bonding of said preformed polyamine through said functional group to said inorganic surface to form a stable inorganic polyamine matrix; and attaching a ligand to said support matrix.
Claim 2
Dependent Claim
85 words
A process for producing a bonded support for a chromatographic system, said process comprising: providing a support structure having an inorganic surface; providing a preformed polyamine; bonding a neutral organosilane to said inorganic surface with said neutral organosilane having a functional group sufficiently reactive so that said preformed polyamine reacts with said functional group to cause covalent bonding of said preformed polyamine through said functional group to said inorganic surface to form a stable inorganic polyamine matrix; and attaching a ligand to said support matrix.
Claim 3
Dependent Claim
25 words
The process of claim 1 wherein said process provides a bonded support for selective partitioning of molecules ranging from about 16 to 10 7 daltons.
Claim 4
Dependent Claim
25 words
The process of claim 1 wherein said support structure is provided by inorganic materials having a pore diameter of between about 100 to 1000 Å.
Claim 5
Dependent Claim
24 words
The process of claim 1 wherein said support structure is provided by inorganic materials of between about 5 to 100 micrometers in particle size.
Claim 6
Dependent Claim
37 words
The process of claim 1 wherein said neutral organosilane provides a thin neutral organic layer on said inorganic surface that eliminates the maximum number of surface silanoles and serves as anchoring means for attachment of said polyamine.
Claim 7
Dependent Claim
18 words
The process of claim 1 wherein said neutral organosilane is one of 2-carbethoxyethyltrichlorosilane, γ-glycidoxypropyl dimethylmethoxysilane and isocyanethyl dimethylchlorosilane.
Claim 8
Dependent Claim
19 words
The process of claim 1 wherein said neutral organosilane is one of a trichlorosilane, a dichlorosilane, and a monochlorosilane.
Claim 9
Dependent Claim
21 words
The process of claim 1 wherein said polyamine is covalently cross-linked to said inorganic surface having said neutral organosilane bonded thereto.
Claim 10
Dependent Claim
16 words
The process of claim 8 wherein said polyamine is one of polyethyleneamine, polyvinylamine, tetraethylenepentamine, or agamatine.
Claim 11
Dependent Claim
15 words
The process of claim 1 wherein said ligand is covalently cross-linked through said support matrix.
Claim 12
Dependent Claim
43 words
The process of claim 10 wherein said ligand is covalently cross-linked through said support matrix to form one of an anion exchange support, a cation exchange support, a size exclusion support, a reverse phase hydrophobic support, an affinity support, and a chelation support.
Claim 13
Dependent Claim
91 words
A process for producing a support matrix for a chromatographic system, said process comprising: providing a support structure having an inorganic surface; providing a preformed organic amine polymer; and bonding a neutral organosilane to said inorganic surface with said neutral organosilane having a functional group sufficiently reactive so that said organic amine polymer reacts with said functional group to cause covalent bonding of said organic amine polymer through said functional group to said inorganic surface to form a stable organic polyamine matrix convertible into a plurality of types of chromatographic supports.
Claim 14
Dependent Claim
91 words
A process for producing a support matrix for a chromatographic system, said process comprising: providing a support structure having an inorganic surface; providing a preformed organic amine polymer; and bonding a neutral organosilane to said inorganic surface with said neutral organosilane having a functional group sufficiently reactive so that said organic amine polymer reacts with said functional group to cause covalent bonding of said organic amine polymer through said functional group to said inorganic surface to form a stable organic polyamine matrix convertible into a plurality of types of chromatographic supports.
Claim 15
Dependent Claim
39 words
The process of claim 12 wherein said process provides a support matrix for one of an anion exchange support, a cation exchange support, a size exclusion support, a reverse phase hydrophobic support, an affinity support, and a chelation support.
Claim 16
Dependent Claim
21 words
The process of claim 12 wherein said organic amine polymer is covalently bonded in a thin layer on said inorganic surface.
Claim 17
Dependent Claim
21 words
The process of claim 12 wherein said functional group is an organic functional group including one of esters, isocyanates and epoxides.
Claim 18
Dependent Claim
93 words
A process for producing a bonded support for a chromatographic system, said process comprising: providing a support structure having an inorganic surface; providing a preformed organic amine polymer; bonding a neutral organosilane to said inorganic surface with said neutral organosilane having a functional group sufficiently reactive so that said organic amine polymer reacts with said functional group to cause covalent bonding of said organic amine polymer through said functional group to said inorganic surface to form a stable organic polyamine matrix; and attaching an organic stationary phase group to said organic polyamine matrix.
Claim 19
Dependent Claim
93 words
A process for producing a bonded support for a chromatographic system, said process comprising: providing a support structure having an inorganic surface; providing a preformed organic amine polymer; bonding a neutral organosilane to said inorganic surface with said neutral organosilane having a functional group sufficiently reactive so that said organic amine polymer reacts with said functional group to cause covalent bonding of said organic amine polymer through said functional group to said inorganic surface to form a stable organic polyamine matrix; and attaching an organic stationary phase group to said organic polyamine matrix.
Claim 20
Dependent Claim
21 words
The process of claim 16 wherein said organic amine polymer is covalently bonded in a thin layer on said inorganic surface.
Claim 21
Dependent Claim
21 words
The process of claim 16 wherein said functional group is an organic functional group including one of esters, isocyanates and epoxides.
Claim 22
Dependent Claim
24 words
The process of claim 16 wherein said organic stationary phase is made by derivating polyamine with an oxirane to form an anion exchange support.
Claim 23
Dependent Claim
45 words
The process of claim 19 wherein said derivating of polyamine is carried out by one of butane monoepoxide, butane diepoxide, butane diol diglycidylether, Epon 812, Epon 828, 1,2-epoxy-ethoxy propane, 1,2-epoxy-3-pentoxy propane, 1,2-epoxy-3-propoxy propane, 1,2-epoxy-3-hexoxy propane, ethane diol diglycidyl ether, ethylene oxide, glycidol, or propylene oxide.
Claim 24
Dependent Claim
30 words
The process of claim 16 wherein said organic stationary phase is made by treating said organic polyamine matrix with cyclic anhydride or 1,3-propane sultone to form a cation exchange support.
Claim 25
Dependent Claim
37 words
The process of claim 21 wherein said organic polyamine matrix is treated with one of 1,2,3,4-cyclopentanetetracarboxylic dianhydride, diglycolic anhydride, dioctylsulfosuccinate sodium salt, dodecene-1-YL-succinic anhydride, ethylene/maleic anhydride copolymer, methylvinyl ether/maleic anhydride copolymer, 1,3-proanesultone/acetic anhydride, succinic anhydride, tetrahydrofuran-2,3,4,5-tetra-carboxylic dianhydride.
Claim 26
Dependent Claim
33 words
The process of claim 16 wherein said inorganic stationary phase is made by treating said organic polyamine matrix with acetic anhydride to form a polyamine acetate coating to form a size exclusion support.
Claim 27
Dependent Claim
35 words
The process of claim 16 wherein said organic stationary phase is made by treating said organic polyamine matrix with the esters or anhydrides or higher carbon chain links to form a reversed phase hydrophobic support.
Claim 28
Dependent Claim
40 words
The process of claim 16 wherein said organic polyamine matrix is treated with one of acetic anhydride, benzoic anhydride, hexanoic anhydride, propionic anhydride, trifluoroacetic anhydride, methyl isocyanate, propyl isocyanate, butyric anhydride to form a size exclusion reversed phase hydrophobic support.
Claim 29
Dependent Claim
29 words
The process of claim 16 wherein said organic stationary phase is made by treating said organic polyamine matrix to produce a primary amine support to form an affinity support.
Claim 30
Dependent Claim
53 words
The process of claim 16 wherein said organic stationary phase is made by treating said organic polyamine matrix with one of acridine, acriflavine, cibacrone blue, conconavalin, lactase, NAD, urease using one of butane diol diglycidyl ether, glutaraldehyde, 1,6-diisocyanate hexane, dimethyl suberimidate hydrochloride, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide as a linking agent to form an affinity support.
Claim 31
Dependent Claim
30 words
The process of claim 16 wherein said organic stationary phase is made by treating said organic polyamine matrix with one of iodocetic acid and pyridine-2-carboxylaldehyde to form a chelation support.
Claim 32
Dependent Claim
20 words
The process of claim 16 wherein said organic polyamine matrix is treated with methyl iodide to form a dimethylaminoethyl support.
Claim 33
Dependent Claim
20 words
The process of claim 16 wherein said organic polyamine matrix is treated with N-acetyl homocyteine-thiolactone to form a sulfhydryl support.
Claim 34
Dependent Claim
118 words
A process for producing a bonded support for a chromatographic system, said process comprising: providing a support structure having an inorganic surface; bonding a neutral organosilane to said inorganic surface, said neutral organosilane having a functional group including one of esters, isocyanates and epoxides; covalently bonding a preformed organic amine polymer to said organic surface to form a stable polyamine matrix, said polymer being one of polyethyleneamine, polyvinylamine, tetraethylenepentamine, and agamatine with said polymer reacting with said functional group of said neutral organosilane to cause said covalent bonding of said polymer through said functional group to said inorganic surface; and covalently bonding an organic stationary phase to said polyamine matrix to form a predetermined type of chromatographic support.
Claim 35
Dependent Claim
118 words
A process for producing a bonded support for a chromatographic system, said process comprising: providing a support structure having an inorganic surface; bonding a neutral organosilane to said inorganic surface, said neutral organosilane having a functional group including one of esters, isocyanates and epoxides; covalently bonding a preformed organic amine polymer to said organic surface to form a stable polyamine matrix, said polymer being one of polyethyleneamine, polyvinylamine, tetraethylenepentamine, and agamatine with said polymer reacting with said functional group of said neutral organosilane to cause said covalent bonding of said polymer through said functional group to said inorganic surface; and covalently bonding an organic stationary phase to said polyamine matrix to form a predetermined type of chromatographic support.
Claim 36
Dependent Claim
17 words
A bonded support for a chromatographic system made by the process as set forth in claim 1.
Claim 37
Dependent Claim
18 words
A bonded support matrix for a chromatographic system made by the process as set forth in claim 12.
References Cited (Backward Citations)
US2728743A
Cited
US3886080A
Cited
US3969261A
Cited
GB1456865A
Cited
US3983299A
Cited
US3998985A
Cited
US4291154A
Cited
US4415631A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-12-23 | 1989-06-24 | 1989-12-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-12-23 | 1993-06-24 | 1993-12-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-12-23 | 1997-06-24 | 1997-12-24 | $7700.00 | Window Closed / Lapsed |