US4583320A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Delivery system for meristematic tissue
Assignee: Monsanto Co
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Filing: 1983-10-25
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Granted: 1986-04-22
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Expires: 2003-10-25
Abstract
Abstract Novel methods and compositions are provided for the delivery of plant meristematic tissue to an environment for growth and development. Isolated meristematic tissue from various sources are combined with beneficial adjuvants in an encapsulating gel matrix which provides a system for the simultaneous delivery of meristematic tissue and adjuvants which can replace true botantic seed.
Inventors
- 👤 M. Keith Redenbaugh
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 18:34
CPC Classifications
A; A01; A01H; A01H4/00; A01H4/005; A01H4/006
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
66 words
A method for delivering meristematic tissue to an environment for growth and development comprising: isolating meristematic tissue substantially free of intact seed coat, which tissue has the potential to differentiate to produce an entire plant body; and encapsulating said isolated meristematic tissue together with at least one adjuvant capable of affecting the meristematic tissue, the resulting plant body or the environment, in a hydrated gel capsule.
Claim 2
Independent Claim
66 words
A method for delivering meristematic tissue to an environment for growth and development comprising: isolating meristematic tissue substantially free of intact seed coat, which tissue has the potential to differentiate to produce an entire plant body; and encapsulating said isolated meristematic tissue together with at least one adjuvant capable of affecting the meristematic tissue, the resulting plant body or the environment, in a hydrated gel capsule.
Claim 3
Dependent Claim
26 words
A method as recited in claim 1 wherein said meristematic tissue is selected from the group consisting of somatic-derived meristematic tissue, zygotic tissue and germline tissue.
Claim 4
Dependent Claim
34 words
A method as recited in claim 1 wherein said adjuvant is selected from the group consisting of pesticides, herbicides, insecticides, fungicides, fumigants, repellents, rodenticides, fertilizers, nutrients, sugars, carbohydrates, ATP, microorganisms, growth regulators and hormones.
Claim 5
Dependent Claim
36 words
A method as recited in claim 1 wherein the meristematic tissue is encapsulated in at least one gel selected from the group consisting of alginate, carrageenan, locust bean gum and gel agents identified in Table 1.
Claim 6
Dependent Claim
62 words
A method as recited in claim 1 wherein the meristematic tissue is encapsulated together with at least one adjuvant selected from the group consisting of bensulide, EPTC, metribuzin, pebulate, Prometryn, pronamide, chlorpropham, alachlor, diazinon, superphosphate, calcium phosphate, potassium phosphate, potassium nitrate, calcium nitrate, ammonium nitrate, starch, sucrose, Rhizobia meliloti, Rhizobia leguminosarum, Azospirillum, Bacillus thuringiensis, Eschericia coli and adjuvants identified in Table 2.
Claim 7
Dependent Claim
39 words
A method as recited in claim 1, further comprising encapsulating said meristematic tissue and each of said adjuvants in distinct regions of the gel whereby the adjuvant, tissue and the environment are allowed to interact in a progressive manner.
Claim 8
Dependent Claim
27 words
A method as recited in claim 6, wherein each distinct region of the gel matrix comprises a gel agent distinct from the gel agents in adjacent regions.
Claim 9
Dependent Claim
20 words
Meristematic tissue substantially free of intact seed coat encapsulated in a hydrated hydrogel capsule together with at least one adjuvant.
Claim 10
Dependent Claim
20 words
Meristematic tissue substantially free of intact seed coat encapsulated in a hydrated hydrogel capsule together with at least one adjuvant.
Claim 11
Dependent Claim
81 words
A meristematic tissue delivery system for delivering totipotent meristematic tissue to an environment for growth and development which comprises: isolated meristematic tissue substantially free of intact seed coat having the potential to differentiate to Produce an entire plant body; at least one adjuvant in a concentration capable of affecting the meristematic tissue, the plant body or the environment; and a hydrated gel matrix encapsulating said meristematic tissue and said adjuvant, whereby said tissue and adjuvant are delivered simultaneously to the environment.
Claim 12
Dependent Claim
81 words
A meristematic tissue delivery system for delivering totipotent meristematic tissue to an environment for growth and development which comprises: isolated meristematic tissue substantially free of intact seed coat having the potential to differentiate to Produce an entire plant body; at least one adjuvant in a concentration capable of affecting the meristematic tissue, the plant body or the environment; and a hydrated gel matrix encapsulating said meristematic tissue and said adjuvant, whereby said tissue and adjuvant are delivered simultaneously to the environment.
Claim 13
Dependent Claim
26 words
A system as recited in claim 9 wherein said meristematic tissue is selected from the group consisting of somatic-derived meristematic tissue, zygotic tissue and germline tissue.
Claim 14
Dependent Claim
40 words
A system as recited in claim 9, the gel matrix further comprising distinct regions of the gel matrix containing each adjuvant and the meristematic tissue, whereby the adjuvant, tissue and the environment are allowed to interact in a progressive manner.
Claim 15
Dependent Claim
27 words
A system as recited in claim 11 wherein aach distinct region of the gel matrix comprises a gel agent distinct from the gel agents in adjacent regions.
Claim 16
Dependent Claim
36 words
A system as recited in claim 9, wherein the meristematic tissue is encapsulated in at least one gel selected from the group consisting of alginate, carrageenan, locust bean gum and gel agents identified in Table 1.
Claim 17
Dependent Claim
62 words
A system as recited in claim 9, wherein the meristematic tissue is encapsulated together with at least one adjuvant selected from the group consisting of bensulide, EPTC, metribuzin, pebulate, protetryn, pronamide, chlorpropham, alachlor, diazinon, superphosphate, calcium phosphate, potassium phosphate, potassium phosphate, calcium nitrate, ammonium nitrate, starch, sucrose, Rhizobia meliloti, Rhizobia leguminosarum, Asospirillum, Bacillus thuringiensis, Eschericia coli and adjuvants identified in Table 2.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-04-21 | 1989-10-21 | 1990-04-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-04-21 | 1993-10-21 | 1994-04-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-04-21 | 1997-10-21 | 1998-04-22 | $7700.00 | Window Closed / Lapsed |