US4585862A
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Fluorescence polarization immunoassay
Assignee: Abbott Laboratories
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Filing: 1984-02-08
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Granted: 1986-04-29
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Expires: 2004-02-08
Abstract
Abstract This disclosure relates to a method and reagents for determining ligands in biological fluids such as serum, plasma, spinal fluid, amnionic fluid and urine. In particular, this disclosure relates to a fluorescence polarization immunoassay procedure and to a novel class of tracer compounds employed as reagents in such procedures. The procedure disclosed combines the specificity of an immunoassay with the speed and convenience of fluorescence polarization techniques to provide a means for determining the amount of the specific ligand present in a sample.
Inventors
- 👤 Chao-Huei J. Wang
- 👤 Stephen D. Stroupe
- 👤 Michael E. Jolley
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 09:42
CPC Classifications
C; C07; C07D; C07D405/00; C07D405/02; C07D405/12; C07D311/00; C07D311/02; C07D311/78; C07D311/80; C07D311/82; C07D311/90; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533
Claims Analysis
Total Claims: 64
Claim 1
Independent Claim
71 words
A compound of the formula ##STR42## wherein R is a ligand-analog of an aromatic carbon-containing ligand having a molecular weight in a range of 100 to 2000 wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody and said ligand-analog has an aromatic carbon by which the ligand-analog is attached to the sulfonamidofluorescein moiety; and biologically acceptable salts thereof.
Claim 2
Dependent Claim
71 words
A compound of the formula ##STR42## wherein R is a ligand-analog of an aromatic carbon-containing ligand having a molecular weight in a range of 100 to 2000 wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody and said ligand-analog has an aromatic carbon by which the ligand-analog is attached to the sulfonamidofluorescein moiety; and biologically acceptable salts thereof.
Claim 3
Dependent Claim
15 words
A compound according to claim 1 of the formula: ##STR43## and biologically acceptable salts thereof.
Claim 4
Dependent Claim
31 words
A compound according to claim 2 wherein R is derived from a ligand selected from the group consisting of steroids, hormones, antiasthmatics, antineoplastics, antiarrhythmics, anticonvulsants, antibiotics; antiarthritics, antidepressants and cardiac glycosides.
Claim 5
Dependent Claim
13 words
A compound according to claim 3 wherein R is derived from an antiarrhythmic.
Claim 6
Dependent Claim
12 words
A compound according to claim 4 wherein R is derived from lidocaine.
Claim 7
Dependent Claim
10 words
The compound of claim 1 having the structural formula ##STR44##
Claim 8
Dependent Claim
13 words
A compound according to claim 3 wherein R is derived from an anticonvulsant.
Claim 9
Dependent Claim
12 words
A compound according to claim 7 wherein R is derived from phenytoin.
Claim 10
Dependent Claim
10 words
A compound according to claim 8 wherein R is ##STR45##
Claim 11
Dependent Claim
12 words
A compound according to claim 7 wherein R is derived from primidone.
Claim 12
Dependent Claim
21 words
A compound according to claim 7 wherein R is ##STR46## wherein R' is alkyl having from one to four carbon atoms.
Claim 13
Dependent Claim
12 words
A compound according to claim 7 wherein R is derived from ethosuccimide.
Claim 14
Dependent Claim
20 words
A compound according to claim 12 wherein R is ##STR47## wherein n is an integer of from 0 to 3.
Claim 15
Dependent Claim
65 words
A compound of the formula ##STR48## wherein R is a ligand-analog having a molecular weight in range of 100 to 2000 wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody and said ligand-analog is attached to the carboamidosulfonylaminofluorescein moiety through a hydroxy oxygen or a reactive amine; and biologically acceptable salts thereof.
Claim 16
Dependent Claim
65 words
A compound of the formula ##STR48## wherein R is a ligand-analog having a molecular weight in range of 100 to 2000 wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody and said ligand-analog is attached to the carboamidosulfonylaminofluorescein moiety through a hydroxy oxygen or a reactive amine; and biologically acceptable salts thereof.
Claim 17
Dependent Claim
15 words
A compound according to claim 14 of the formula ##STR49## and biologically acceptable salts thereof.
Claim 18
Dependent Claim
31 words
A compound according to claim 15 wherein R is derived from a ligand selected from the group consisting of steroids, hormones, antiasthmatics, antineoplastics, antiarrhythmics, anticonvulsants, antibiotics, antiarthritics, antidepressants and cardiac glycosides.
Claim 19
Dependent Claim
13 words
A compound according to claim 16 wherein R is derived from an anticonvulsant.
Claim 20
Dependent Claim
12 words
A compound according to claim 17 wherein R is derived from carbamazepine.
Claim 21
Dependent Claim
9 words
A compound of the formula wherein R is ##STR50##
Claim 22
Dependent Claim
9 words
A compound of the formula wherein R is ##STR50##
Claim 23
Dependent Claim
13 words
A compound according to claim 16 wherein R is derived from an antiarrhythmic.
Claim 24
Dependent Claim
12 words
A compound according to claim 20 wherein R is derived from procainamide.
Claim 25
Dependent Claim
12 words
A compound according to claim 20 wherein R is derived from propranolol.
Claim 26
Dependent Claim
66 words
A compound of the formula ##STR51## wherein R is a ligand-analog having a molecular weight in a range of 100 to 2000 wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody and said ligand-analog has a reactive nitrogen by which the ligand-analog is attached to the oxalylaminofluorescein moiety; and biologically acceptable salts thereof.
Claim 27
Dependent Claim
66 words
A compound of the formula ##STR51## wherein R is a ligand-analog having a molecular weight in a range of 100 to 2000 wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody and said ligand-analog has a reactive nitrogen by which the ligand-analog is attached to the oxalylaminofluorescein moiety; and biologically acceptable salts thereof.
Claim 28
Dependent Claim
11 words
A compound of the formula: ##STR52## and biologically acceptable salts thereof.
Claim 29
Dependent Claim
11 words
A compound of the formula: ##STR52## and biologically acceptable salts thereof.
Claim 30
Dependent Claim
31 words
A compound according to claim 24 wherein R is derived from a ligand selected from the group consisting of steroids, hormones, antiasthmatics, antineoplastics, antiarrhythmics, anticonvulsants, antibiotics, antiarthritics, antidepressants and cardiac glycosides.
Claim 31
Dependent Claim
13 words
A compound according to claim 25 wherein R is derived from an antidepressant.
Claim 32
Dependent Claim
14 words
A compound according to claim 26 wherein R is derived from a tricyclic drug.
Claim 33
Dependent Claim
16 words
A compound according to claim 27 wherein R is derived from nortriptyline, amitriptyline, imipramine or desipramine.
Claim 34
Dependent Claim
13 words
A compound according to claim 28 wherein R is derived from an anticonvulsant.
Claim 35
Dependent Claim
12 words
A compound according to claim 29 wherein R is derived from carbamazepine.
Claim 36
Dependent Claim
10 words
A compound according to claim 30 wherein R is ##STR53##
Claim 37
Independent Claim
116 words
A method for determining ligands in a sample comprising intermixing with said sample a biologically acceptable salt of a tracer of the formula: ##STR54## wherein R is a ligand-analog having a molecular weight in a range of 100 to 2000 wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody and said ligand-analog has an aromatic carbon by which the ligand-analog is attached to the sulfonamidofluorescein moiety; and an antibody capable of specifically recognizing said ligand and said tracer and then determining the amount of tracer bound to antibody by fluorescence polarization techniques as a measure of the amount of ligand in the sample.
Claim 38
Independent Claim
116 words
A method for determining ligands in a sample comprising intermixing with said sample a biologically acceptable salt of a tracer of the formula: ##STR54## wherein R is a ligand-analog having a molecular weight in a range of 100 to 2000 wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody and said ligand-analog has an aromatic carbon by which the ligand-analog is attached to the sulfonamidofluorescein moiety; and an antibody capable of specifically recognizing said ligand and said tracer and then determining the amount of tracer bound to antibody by fluorescence polarization techniques as a measure of the amount of ligand in the sample.
Claim 39
Dependent Claim
27 words
A method according to claim 32 wherein the sulfonamido group bonded to the R group is also bonded to the 4- or 5-position of the fluorescein moiety.
Claim 40
Dependent Claim
26 words
A method according to claim 33 wherein said ligand is a steroid, hormone, antiasthmatic, antineoplastic, antiarrhythmic, anticonvulsant, antibiotic, antiarthritic, antidepressant, cardiac glycoside or a metabolite thereof.
Claim 41
Dependent Claim
19 words
A method according to claim 34 wherein R has a molecular weight in a range of 50 to 4000.
Claim 42
Dependent Claim
19 words
A method according to claim 35 wherein R has a molecular weight in a range of 100 to 2000.
Claim 43
Dependent Claim
12 words
A method according to claim 36 wherein said ligand is an antiarrhythmic.
Claim 44
Dependent Claim
11 words
A method according to claim 37 wherein said antiarrhythmic is lidocaine.
Claim 45
Dependent Claim
12 words
A method according to claim 38 wherein said ligand is an anticonvulsant.
Claim 46
Dependent Claim
11 words
A method according to claim 39 wherein said anticonvulsant is phenytoin.
Claim 47
Dependent Claim
11 words
A method according to claim 39 wherein said anticonvulsant is primidone.
Claim 48
Dependent Claim
11 words
A method according to claim 39 wherein said anticonvulsant is ethosuximide.
Claim 49
Independent Claim
85 words
A method for determining ligands in a sample comprising intermixing with said sample a biologically acceptable salt of a tracer of the formula: ##STR55## wherein R is a ligand-analog having a molecular weight in a range of 100 to 2000 wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody and said ligand-analog is attached to the carboamidosulfonylamino group moiety through a hydroxy oxygen or a reactive amine; and biologically acceptable salts thereof.
Claim 50
Independent Claim
85 words
A method for determining ligands in a sample comprising intermixing with said sample a biologically acceptable salt of a tracer of the formula: ##STR55## wherein R is a ligand-analog having a molecular weight in a range of 100 to 2000 wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody and said ligand-analog is attached to the carboamidosulfonylamino group moiety through a hydroxy oxygen or a reactive amine; and biologically acceptable salts thereof.
Claim 51
Dependent Claim
27 words
A method according to claim 43 wherein the carboamidosulfonylamino group bonded to the R group is also bonded to the 4- or 5-position of the fluorescein moiety.
Claim 52
Dependent Claim
26 words
A method according to claim 44 wherein said ligand is a steroid, hormone, antiasthmatic, antineoplastic, antiarrhythmic, anticonvulsant, antibiotic, antiarthritic, antidepressant, cardiac glycoside or a metabolite thereof.
Claim 53
Dependent Claim
19 words
A method according to claim 45 wherein R has a molecular weight in a range of 50 to 4000.
Claim 54
Dependent Claim
19 words
A method according to claim 46 wherein R has a molecular weight in a range of 100 to 2000.
Claim 55
Dependent Claim
12 words
A method according to claim 47 wherein said ligand is an anticonvulsant.
Claim 56
Dependent Claim
11 words
A method according to claim 48 wherein said anticonvulsant is carbamazepine.
Claim 57
Independent Claim
84 words
A method for determining ligands in a sample comprising intermixing with said sample a biologically acceptable salt of a tracer of the formula: ##STR56## wherein R is a ligand-analog having a molecular weight in a range of 100 to 2000 wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody and said ligand-analog has a reactive nitrogen by which the ligand-analog is attached to the oxalylaminofluorescein moiety; and biologically acceptable salts thereof.
Claim 58
Independent Claim
84 words
A method for determining ligands in a sample comprising intermixing with said sample a biologically acceptable salt of a tracer of the formula: ##STR56## wherein R is a ligand-analog having a molecular weight in a range of 100 to 2000 wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody and said ligand-analog has a reactive nitrogen by which the ligand-analog is attached to the oxalylaminofluorescein moiety; and biologically acceptable salts thereof.
Claim 59
Dependent Claim
27 words
A method according to claim 50 wherein the oxalylamino group bonded to the R group is also bonded to the 4- or 5-position of the fluorescein moiety.
Claim 60
Dependent Claim
26 words
A method according to claim 51 wherein said ligand is a steroid, hormone, antiashtmatic, antineoplastic, antiarrhythmic, anticonvulsant, antibiotic, antiarthritic, antidepressant, cardiac glycoside or a metabolite thereof.
Claim 61
Dependent Claim
19 words
A method according to claim 52 wherein R has a molecular weight in a range of 50 to 4000.
Claim 62
Dependent Claim
19 words
A method according to claim 53 wherein R has a molecular weight in a range of 100 to 2000.
Claim 63
Dependent Claim
12 words
A method according to claim 54 wherein said ligand is an anticonvulsant.
Claim 64
Dependent Claim
11 words
A method according to claim 55 wherein said anticonvulsant is carbamazepine.
References Cited (Backward Citations)
JPS56121039A
Cited
US4476229A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-04-28 | 1989-10-28 | 1990-04-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-04-28 | 1993-10-28 | 1994-04-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-04-28 | 1997-10-28 | 1998-04-29 | $7700.00 | Window Closed / Lapsed |