US4587624A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and apparatus for correcting a curve relating an absorbance to a concentration
Assignee: Olympus Corp
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Filing: 1982-12-20
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Granted: 1986-05-06
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Expires: 2002-12-20
Abstract
Abstract In a colorimetric measurement using a non-linear curve relating an absorbance to a concentration of substance to be measured, the non-linear curve is corrected by measuring an absorbance value y 6 ' of only one of six standard samples of known different concentrations x 1 , x 2 . . . x 6 which are used for making the initial non-linear curve, deriving a quotient α by dividing the newly measured absorbance value y 6 ' by a previously measured absorbance value y 6 , deriving corrected absorbance values y 1 ', y 2 ' . . . y 5 ' by producing products αy 1 , αy 2 . . . αy 5 of the quotient α and previously measured absorbance values y 1 , y 2 . . . y 5 , and by forming a corrected non-linear curve with the aid of the corrected absorbance values y 1 ', y 2 ' . . . y 5 ' and the newly measured absorbance value y 6 '.
Inventors
- 👤 Taiichi Banno
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 19:38
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/17; G01N21/25; G01N21/27; G01N21/274; G01N21/59
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
103 words
A method of determining the concentration of an unknown sample having a non-linear curve relating absorbance to concentration of the unknown sample comprising the steps of: forming said non-linear curve by using at least three standard samples having known different concentrations; measuring an absorbance value of only one standard sample of said at least three standard samples having known different concentrations; correcting the non-linear curve on the basis of said measured absorbance value; measuring the absorbance value of said unknown sample; and deriving a concentration for said unknown sample from said corrected non-linear curve using the measured absorbance value of said unknown sample.
Claim 2
Independent Claim
103 words
A method of determining the concentration of an unknown sample having a non-linear curve relating absorbance to concentration of the unknown sample comprising the steps of: forming said non-linear curve by using at least three standard samples having known different concentrations; measuring an absorbance value of only one standard sample of said at least three standard samples having known different concentrations; correcting the non-linear curve on the basis of said measured absorbance value; measuring the absorbance value of said unknown sample; and deriving a concentration for said unknown sample from said corrected non-linear curve using the measured absorbance value of said unknown sample.
Claim 3
Dependent Claim
136 words
A method according to claim 1, wherein said correcting step comprises: deriving a difference between the previously determined absorbance value y n and the newly measured absorbance value y n ' of the same standard sample; deriving a quotient β by dividing said difference y n -y n ' by the known concentration x n of the relevant standard sample; deriving products of the quotient β and the known concentrations x 1 , x 2 , . . . x n of the standard samples; and deriving differences between the previously determined absorbance values y 1 , y 2 , . . . y n and said products βx 1 , βx 2 , . . . βx n to produce corrected absorbance values y 1 ', y 2 ', . . . y n '.
Claim 4
Dependent Claim
33 words
A method according to claim 2, wherein said newly measured absorbance value y n ' is obtained by measuring the absorbance value of the standard sample having the highest concentration x n .
Claim 5
Dependent Claim
86 words
A method according to claim 1, wherein said correcting step comprises: deriving a quotient α by dividing the newly measured absorbance value y n ' of the standard sample by the previously determined absorbance value y n of the same standard sample; and deriving products of the quotient α and previously determined absorbance values y 1 , y 2 , . . . y n of the standard samples to produce corrected absorbance values y 1 ', y 2 ', . . . y n '.
Claim 6
Dependent Claim
27 words
A method according to claim 4, wherein said newly measured absorbance value is derived by measuring an absorbance value of the standard sample having the highest concentration.
Claim 7
Dependent Claim
33 words
A method according to claim 4, wherein said correcting step further comprises: dividing intervals between successive concentrations into sections having widths g 1 , g 2 , . . . g p .
Claim 8
Dependent Claim
15 words
A method according to claim 4, wherein said quotient is corrected from an external input.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-05-05 | 1989-11-04 | 1990-05-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-05-05 | 1993-11-04 | 1994-05-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-05-05 | 1997-11-04 | 1998-05-06 | $7700.00 | Window Closed / Lapsed |