Purification and activity assurance of precipitated heterologous proteins

Claim 2

A process for re-activating proteins formed as refractile bodies in host cells, said proteins not subjected to CNBR treatment which process comprises: (a) converting said proteins not subjected to CNBR treatment and dissolved in a strongly denaturing solution to the corresponding protein-S-sulfonate; (b) converting the strongly denaturing solution to a weakly denaturing solution in which the protein-S-sulfonate remains dissolved; and (c) treating the resulting protein-S-sulfonate with a sulfhydryl containing moiety containing a small percentage of its disulfide.

Claim 3

The process of claim 1 wherein the weakly denaturing solution is 1-9M in urea.

Claim 4

The process of claim 1 wherein the strongly denaturing solution is 4 to 9M in a guanidine salt, and the weakly denaturing solution is 1 to 9M in urea or 0.5 to 2M in guanidine hydrochloride.

Claim 5

The process of claim 1 wherein the sulfhydryl containing moiety is reduced glutathione.

Claim 6

The process of claim 4 whwerein the dissolved protein is converted to the protein-S-sulfonate in (a) by treating the solution with a mixture of a sulfite and a weak oxidizing agent.

Claim 7

The process of claim 1 wherein the sulfite is sodium sulfite and the weak oxidizing agent is sodium tetrathionate.

Claim 8

A process for re-activating heterologous proteins formed as refractile bodies in host cells said proteins not subjected to CNBR treatment which process comprises: (a) converting said proteins not subjected to CNBR treatment and dissolved in a strongly denaturing solution to the corresponding protein-S-sulfonate; (b) replacing the strongly denaturing solution with a weakly denaturing solution; (c) subjecting the dissolved protein-S-sulfonate to one or more purification procedures selected from the group consisting of ion exchange chromatography and gel permeation chromatography; and (d) treating the purified protein-S-sulfonate from (c) with a sulfhydryl reagent containing a small percentage of its disulfide form.

Claim 9

A process for re-activating heterologous proteins formed as refractile bodies in host cells said proteins not subjected to CNBR treatment which process comprises: (a) converting said proteins not subjected to CNBR treatment and dissolved in a strongly denaturing solution to the corresponding protein-S-sulfonate; (b) replacing the strongly denaturing solution with a weakly denaturing solution; (c) subjecting the dissolved protein-S-sulfonate to one or more purification procedures selected from the group consisting of ion exchange chromatography and gel permeation chromatography; and (d) treating the purified protein-S-sulfonate from (c) with a sulfhydryl reagent containing a small percentage of its disulfide form.

Claim 10

The process of claim 7 wherein the strongly denaturing solution is 4 to 9M in a guanidine salt and the weakly denaturing solution is 1 to 9M in urea or 0.5 to 2M in guanidine hydrochloride.

Claim 11

The process of claim 7 wherein the dissolved protein is converted to the protein-S-sulfonate in step (a) by treating the solution with a mixture of sulfite and weak oxidizing agent.

Claim 12

The process of claim 7 which includes carrying out a gel permeation chromatography step in (c).

Claim 13

The process of claim 7 which includes carrying out ion exchange chromatography in step (c).

Claim 14

The method of claim 7 wherein the purification procedure includes the use of ion exchange chromatography comprising DEAE cellulose.

Claim 15

The method of claim 12 wherein the DEAE cellulose absorbs contaminants of the heterologous protein, but not the heterologous protein.

Claim 16

Protein reactivated by the process of claim 1.

Claim 17

Protein reactivated by the process of claim 7.

Claim 18

The process of claim 1 including removing any remaining denaturant from the solution.

Claim 19

The process of claim 7 including removing substantially all of the remaining denaturant.