Element for electrophoresis

Claim 1

An element for electrophoresis comprising the following three-layer structure laminated in the order: (I) a support layer; (II) an adhesive layer comprising a polymer having at least one repeating unit selected from the group consisting of: (1) a repeating unit having the formula (1): ##STR17## in which R 11 is the hydrogen atom or an alkyl group containing 1-6 carbon atoms; Q 1 is --COO--, --CON(R 11 )--, or an arylene group containing 6-10 carbon atoms; L 1 is a divalent group containing at least one linkage selected from the group consisting of --COO-- and --CON(R 11 )-- and containing 3-15 carbon atoms, or a divalent group containing at least one linkage selected from the group consisting of --O--, --N(R 11 )--, --CO--, --SO--, --SO 2 --, --SO 3 --, --SO 2 N(R 11 )--, --N(R 11 )CON(R 11 )-- and --N(R 11 )COO--, and containing 1-12 carbon atoms, in which R 11 has the same meaning as defined above; R 12 is --CH═CH 2 or --CH 2 CH 2 X 1 , in which X 1 is a substituent replaceable with a nucleophilic group or releasable in the form of HX 1 by a base; A 1 is a divalent group derived from an ethylenic unsaturated monomer copolymerizable with monomers forming other unit portions; and x 1 , y 1 and z 1 all representing molar percents range from 0 to 99, from 1 to 99, and from 1 to 99, respectively; (2) a repeating unit having the formula (2): ##STR18## in which R 21 is the hydrogen atom or an alkyl group containing 1-6 carbon atoms; R 22 is --CH═CH 2 or --CH 2 CH 2 X 2 , in which X 2 is a substituent replaceable with a nucleophilic group or releasable in the form of HX 2 by a base; L 2 is a divalent group selected from the group consisting of an alkylene group containing 1-6 carbon atoms, an arylene group containing 6-12 carbon atoms, --COZ 2 --, and --COZ 2 R 23 --, in which R 23 is an alkylene group containing 1-6 carbon atoms, or an arylene group containing 6-12 carbon atoms, and Z 2 is the oxygen atom or NH; A 2 is a divalent group derived from an ethylenic unsaturated monomer copolymerizable with monomers forming other unit portions; and x 2 , y 2 and z 2 all representing molar percents range from 0 to 99, from 1 to 99, and from 1 to 99, respectively; and (3) a repeating unit having the formula (3): ##STR19## in which R 31 is the hydrogen atom or an alkyl group containing 1-6 carbon atoms; L 3 is a divalent linkage group containing 1-20 carbon atoms; X 3 is an active ester; A 3 is a divalent group derived from an ethylenic unsaturated monomer copolymerizable with monomers forming other unit portions; and x 3 , y 3 and z 3 all representing molar percents range from 0 to 99, from 1 to 99, and from 1 to 99, respectively; and m is 0 or 1; and (III) a medium layer for electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound, a crosslinking agent in the presence of water and a water-soluble polymer and agarose.