US4622298A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Detection and quantitation of microorganisms, leukocytes and squamous epithelial cells in urine
Assignee: Becton Dickinson and Co
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Filing: 1984-08-09
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Granted: 1986-11-11
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Expires: 2004-08-09
Abstract
Abstract A method for the assessment of bacteriuria and pyuria includes the simultaneous detection and quantitation of microorganisms, leukocytes and squamous epithelial cells in a urine specimen. The three cell types are stained with a fluorescent dye, and the urine specimen is analyzed directly, preferably by a single flow microfluorometry protocol.
Inventors
- 👤 James D. Mansour
- 👤 Thomas H. Schulte
- 👤 Burton H. Sage
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 20:03
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56966; G01N33/56972; C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/06
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
50 words
A method for the assessment of bacteriuria and pyuria comprising contacting a urine specimen with a staining composition including a fluorescent dye and a staining buffer to provide a mixture and simultaneously detecting and quantitating microorganisms, leukocytes and squamous epithelial cells in said mixture by a single flow microfluorometry protocol.
Claim 2
Independent Claim
50 words
A method for the assessment of bacteriuria and pyuria comprising contacting a urine specimen with a staining composition including a fluorescent dye and a staining buffer to provide a mixture and simultaneously detecting and quantitating microorganisms, leukocytes and squamous epithelial cells in said mixture by a single flow microfluorometry protocol.
Claim 3
Dependent Claim
34 words
The method in accordance with claim 1 wherein said microorganisms are selected from the group of microorganisms consisting of Proteus mirabilis, Staphylococcus saprophyticus, Streptococcus fecalis, Lactobacillus casei, Escherichia coli, Klebsiella pneumoniae, and Pseudomonus aeruginosa.
Claim 4
Dependent Claim
28 words
The method in accordance with claim 1 wherein said fluorescent dye is selected from the group of dyes consisting of ethidium bromide, thioflavin T, DAPI and 3,3'-dipentyloxacarbocyanine iodide.
Claim 5
Dependent Claim
16 words
The method in accordance with claim 1 wherein said composition includes a plurality of fluorescent dyes.
Claim 6
Dependent Claim
23 words
The method in accordance with claim 1 wherein said staining buffer comprises sodium borate, ethylenediamine tetraacetic acid, formaldehyde and a surface active agent.
Claim 7
Dependent Claim
16 words
The method in accordance with claim 5 wherein said surface active agent is octyl phenoxy polyethoxyethanol.
Claim 8
Dependent Claim
21 words
The method in accordance with claim 1 wherein said flow microfluorometry protocol includes obtaining multiple parameter measurements of the urine specimen.
Claim 9
Dependent Claim
29 words
The method in accordance with claim 7 wherein said parameter measurements include at least two of forward light scatter, 90° light scatter, red fluorescence emission and green fluorescence emission.
Claim 10
Dependent Claim
18 words
The method in accordance with claim 7 wherein said multiple parameter measurements are taken using a logarithmic scale.
Claim 11
Dependent Claim
17 words
The method in accordance with claim 9 wherein a single setting of the flow microfluorometer is used.
Claim 12
Independent Claim
81 words
A method for the assessment of bacteriuria and pyuria by analysis of a urine sample for the presence of microorganisms, leukocytes and squamous epithelial cells comprising contacting a urine sample with a staining composition including ethidium bromide and a staining buffer to provide a mixture, incubating said mixture, and analyzing said mixture by a flow microfluorometry protocol including logarithmic scale measurement of forward and 90° light scattering and red and green fluorescence emission after a single setting of the flow microfluorometer.
Claim 13
Independent Claim
81 words
A method for the assessment of bacteriuria and pyuria by analysis of a urine sample for the presence of microorganisms, leukocytes and squamous epithelial cells comprising contacting a urine sample with a staining composition including ethidium bromide and a staining buffer to provide a mixture, incubating said mixture, and analyzing said mixture by a flow microfluorometry protocol including logarithmic scale measurement of forward and 90° light scattering and red and green fluorescence emission after a single setting of the flow microfluorometer.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-11-10 | 1990-05-12 | 1990-11-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-11-10 | 1994-05-12 | 1994-11-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-11-10 | 1998-05-12 | 1998-11-11 | $7700.00 | Window Closed / Lapsed |