US4637834A
Expired / Lapsed
US
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Aqueous protein solutions which are stable towards denaturing, processes for their preparation and their use
Assignee: Sanofi Aventis Deutschland GmbH
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Filing: 1986-02-05
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Granted: 1987-01-20
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Expires: 2006-02-05
Abstract
Abstract The invention relates to aqueous solutions of proteins with a molecular weight above 8,500 daltons, which contain a compound of the formula I R.sup.2 O--X.sub.n --R.sup.3 (I) in which X n is a chain of n members of the formula II or III ##STR1## in any desired sequence, n denotes 2 to 200 and R 1 denotes hydrogen, methyl or ethyl, it being possible for the radicals R 1 to be identical or different but R 1 being hydrogen in at least half of the chain members, and R 2 and R 3 are identical or different and denote hydrogen or an organic radical, to processes for their preparation and to their use. The invention furthermore relates to the use of compounds of the formula I for the pretreatment of hydrophobic surfaces to avoid adsorption of denaturing of proteins.
Inventors
- 👤 Horst Thurow
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 11:00
CPC Classifications
A; A61; A61K; A61K38/00; A61K38/16; C; C12; C12N; C12N9/00; C12N9/96; A61K38/41; A61K38/42; A61K38/43; A61K38/46; A61K38/47; A61K39/00; A61K39/395; A61K39/39591; A61K47/00; A61K47/06; A61K47/08; A61K47/10; C12Y; C12Y302/00; C12Y302/01; C12Y302/01023
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
122 words
An aqueous solution of a protein with a molecular weight above 8,500 daltons, which contains a compound of the formula I R.sup.2 O--X.sub.n --R.sup.3 (I) in which X n is a chain of n members of the formula II or III ##STR4## in any desired sequence, n denotes 2 to 200, preferably 4 to 100 and R 1 denotes hydrogen, methyl or ethyl, it being possible for the radicals R 1 to be identical or different, but R 1 being hydrogen in more than 75% of the chain members, and R 2 and R 3 are identical or different and denote hydrogen or an organic radical, such that the protein is protected from adsorption at interfaces and protected against denaturing and precipitation.
Claim 2
Dependent Claim
122 words
An aqueous solution of a protein with a molecular weight above 8,500 daltons, which contains a compound of the formula I R.sup.2 O--X.sub.n --R.sup.3 (I) in which X n is a chain of n members of the formula II or III ##STR4## in any desired sequence, n denotes 2 to 200, preferably 4 to 100 and R 1 denotes hydrogen, methyl or ethyl, it being possible for the radicals R 1 to be identical or different, but R 1 being hydrogen in more than 75% of the chain members, and R 2 and R 3 are identical or different and denote hydrogen or an organic radical, such that the protein is protected from adsorption at interfaces and protected against denaturing and precipitation.
Claim 3
Dependent Claim
78 words
A aqueous protein solution as claimed in claim 1, in which R 2 and R 3 independently of one another denote hydrogen, an aliphatic radical with 1 to 20 carbon atoms, an alicyclic radical with 3 to 10 carbon atoms, an alicyclic-aliphatic radical with 4 to 20 carbon atoms, an aliphatic ester group with 2 to 20 carbon atoms, an arylaliphatic radical with 7 to 20 carbons atoms or an aryl radical with 6 to 20 carbon atoms.
Claim 4
Dependent Claim
44 words
An aqueous protein solution as claimed in claim 1, in which R 2 and R 3 independently of one another denote hydrogen, alkyl with 1 to 20 carbon atoms, alkanoyl with 2 to 20 carbon atoms or alkylphenyl with 1 to 10 alkylcarbon atoms.
Claim 5
Dependent Claim
23 words
An aqueous protein solution as claimed in claim 1, which contains a mixture of at least two different compounds of the formula I.
Claim 6
Dependent Claim
27 words
An aqueous protein solution as claimed in claim 1, which contains customary additives for adjusting the isotonicity, for preservation, for buffering and/or for achieving a depot action.
Claim 7
Dependent Claim
16 words
An aqueous protein solution as claimed in claim 1, which contains at least two different proteins.
Claim 8
Dependent Claim
29 words
An aqueous solution as claimed in claim 1 where the concentration of the compound of formula I is between 0.01% and 0.2% (based on the weight of the solution).
Claim 9
Dependent Claim
115 words
A medicament suitable for administration by metering units comprising an aqueous solution of a protein with a molecular weight above 8,500 daltons, which contains a compound of the formula I R.sup.2 O--X.sub.n --R.sup.3 (I) in which X n is a chain of n members of the formula II or III ##STR5## in any desired sequence, n denotes 2 to 200, preferably 4 to 100, and R 1 denotes hydrogen, methyl or ethyl, it being possible for the radicals R 1 to be identical or different, but R 1 being hydrogen in more than half of the chain members, and R 2 and R 3 are identical or different and denote hydrogen or an organic radical.
Claim 10
Dependent Claim
115 words
A medicament suitable for administration by metering units comprising an aqueous solution of a protein with a molecular weight above 8,500 daltons, which contains a compound of the formula I R.sup.2 O--X.sub.n --R.sup.3 (I) in which X n is a chain of n members of the formula II or III ##STR5## in any desired sequence, n denotes 2 to 200, preferably 4 to 100, and R 1 denotes hydrogen, methyl or ethyl, it being possible for the radicals R 1 to be identical or different, but R 1 being hydrogen in more than half of the chain members, and R 2 and R 3 are identical or different and denote hydrogen or an organic radical.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-01-19 | 1990-07-21 | 1991-01-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-01-19 | 1994-07-21 | 1995-01-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-01-19 | 1998-07-21 | 1999-01-20 | $7700.00 | Window Closed / Lapsed |