US4659666A
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Pure alkaline phosphatase, its preparation and use
Assignee: INTERNATIONALE OCTROI MATSCHAPPIJ "OCTROPA" BV A Co OF NETHERLANDS
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Filing: 1984-10-26
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Granted: 1987-04-21
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Expires: 2004-10-26
Abstract
Abstract The enzyme alkaline phosphatase having the following properties: (i) molecular weight: approximately 80 000 with 2 subunits of 40 000 (iii) activator: Mg ++ inhibitor: EDTA (iii) thermal stability: 45° C. or below (iv) optimum temperature: 35°-45° C. (v) pH stability: 6-11 (vi) pH optimum: 10 (vii) specific activity: 3000-5000 units/mg (viii) isoelectric range: between pH 5.0 and 6.0. An antibody-enzyme conjugate process for purifying alkaline phosphatase and a reagent test kit using the purified alkaline phosphatase are also disclosed.
Inventors
- 👤 Keith May
- 👤 Mohamed M. Gani
- 👤 Stephanie J. Senior
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 16:09
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/16; C07; C07K; C07K16/00; C07K16/18; C07K16/26; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/814; Y10S435/815
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
63 words
The enzyme alkaline phosphatase which has the following physical and chemical properties: (i) molecular weight: approximately 80 000 with 2 subunits of 40 000 (ii) activator: Mg ++ inhibitor: EDTA (iii) thermal stability: 45° C. or below (iv) optimum temperature: 35°-45° C. (v) pH stability: 6-11 (vi) pH optimum: 10 (vii) specific activity: 3000-5000 units/mg (viii) isoelectric range: between pH 5.0 and 6.0.
Claim 2
Dependent Claim
63 words
The enzyme alkaline phosphatase which has the following physical and chemical properties: (i) molecular weight: approximately 80 000 with 2 subunits of 40 000 (ii) activator: Mg ++ inhibitor: EDTA (iii) thermal stability: 45° C. or below (iv) optimum temperature: 35°-45° C. (v) pH stability: 6-11 (vi) pH optimum: 10 (vii) specific activity: 3000-5000 units/mg (viii) isoelectric range: between pH 5.0 and 6.0.
Claim 3
Dependent Claim
25 words
Alkaline phosphatase according to claim 1 characterized in that the specific activity is from 3500-4500 units/mg and the isoelectric range between pH 5.0 and 5.5.
Claim 4
Dependent Claim
13 words
An antibody-enzyme conjugate wherein the enzyme is the alkaline phosphatase of claim 1.
Claim 5
Dependent Claim
14 words
The antibody-enzyme conjugate of claim 3 wherein the antibody is anti-beta human chorionic gonadotropin.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-04-20 | 1990-10-20 | 1991-04-21 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-04-20 | 1994-10-20 | 1995-04-21 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-04-20 | 1998-10-20 | 1999-04-21 | $7700.00 | Window Closed / Lapsed |