US4690891A
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Method and the microorganism and enzyme used therein for degrading the xanthan molecule
Assignee: ExxonMobil Technology and Engineering Co
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Filing: 1985-09-11
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Granted: 1987-09-01
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Expires: 2005-09-11
Abstract
Abstract The present invention is a method and the microorganism and enzyme used therein to degrade the Xanthan molecule. The microorganism is a soil bacterium, Bacillus sp. The method includes using the mixed culture, or a supernatant derived therefrom or the purified enzyme itself.
Inventors
- 👤 Ching-Tsang Hou
- 👤 Nancy P. Barnabe
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 21:06
CPC Classifications
C; C12; C12P; C12P19/00; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12P39/00; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
71 words
A method for degrading xanthan molecule comprising contacting said xanthan molecule with a mixed culture bearing the ARS Culture Collection Accession No. NRRL B-15992, wherein the contacting is conducted under aerobic conditions suitable for the growth of said first soil bacterium and for the elaboration of xanthan-degrading enzymes by said first soil bacterium, wherein said xanthan-degrading enzymes include a xanthan depolymerase which breaks the endo β-1,4-glucosidic linkage of said xanthan molecule.
Claim 2
Independent Claim
71 words
A method for degrading xanthan molecule comprising contacting said xanthan molecule with a mixed culture bearing the ARS Culture Collection Accession No. NRRL B-15992, wherein the contacting is conducted under aerobic conditions suitable for the growth of said first soil bacterium and for the elaboration of xanthan-degrading enzymes by said first soil bacterium, wherein said xanthan-degrading enzymes include a xanthan depolymerase which breaks the endo β-1,4-glucosidic linkage of said xanthan molecule.
Claim 3
Independent Claim
92 words
A method for producing a supernatant which includes a salt-tolerant xanthan depolymerase comprising: a. culturing on a xanthan molecule-containing medium a mixed culture obtained from a culture bearing the ARS Culture Collection Accession No. NRRL B-15992, wherein said culturing is conducted under aerobic conditions suitable for the growth of said first soil bacterium and for the elaboration of xanthan depolymerase by said first soil bacterium; and b. recovering said supernatant which includes a xanthan depolymerase from said medium, said xanthan depolymerase capable of breaking the endo β-1,4-glucosidic linkage of said xanthan molecule.
Claim 4
Independent Claim
92 words
A method for producing a supernatant which includes a salt-tolerant xanthan depolymerase comprising: a. culturing on a xanthan molecule-containing medium a mixed culture obtained from a culture bearing the ARS Culture Collection Accession No. NRRL B-15992, wherein said culturing is conducted under aerobic conditions suitable for the growth of said first soil bacterium and for the elaboration of xanthan depolymerase by said first soil bacterium; and b. recovering said supernatant which includes a xanthan depolymerase from said medium, said xanthan depolymerase capable of breaking the endo β-1,4-glucosidic linkage of said xanthan molecule.
Claim 5
Dependent Claim
16 words
The method as described in claim 2 wherein said mixed culture is said culture NRRL B-15992.
Claim 6
Independent Claim
83 words
A method for producing a salt-tolerant xanthan depolymerase comprising: a. culturing a xanthan molecule-containing medium a mixed culture bearing the ARS Culture Collection Accession No. NRRL B-15992, wherein said culturing is conducted under aerobic conditions suitable for the growth of said first soil bacterium and for the elaboration of xanthan depolymerase by said first soil bacterium; and b. recovering said supernatant which includes a xanthan depolymerase from said medium, said xanthan depolymerase capable of breaking the endo β-1,4-glucosidic linkage of said xanthan molecule.
Claim 7
Independent Claim
83 words
A method for producing a salt-tolerant xanthan depolymerase comprising: a. culturing a xanthan molecule-containing medium a mixed culture bearing the ARS Culture Collection Accession No. NRRL B-15992, wherein said culturing is conducted under aerobic conditions suitable for the growth of said first soil bacterium and for the elaboration of xanthan depolymerase by said first soil bacterium; and b. recovering said supernatant which includes a xanthan depolymerase from said medium, said xanthan depolymerase capable of breaking the endo β-1,4-glucosidic linkage of said xanthan molecule.
Claim 8
Dependent Claim
26 words
An isolated and biologically pure microbial culture of Bacillus sp. and mutants thereof obtained from the culture bearing the ARS Culture Collection Accession No. NRRL B-15992.
Claim 9
Dependent Claim
26 words
An isolated and biologically pure microbial culture of Bacillus sp. and mutants thereof obtained from the culture bearing the ARS Culture Collection Accession No. NRRL B-15992.
Claim 10
Dependent Claim
18 words
An isolated and biologically pure culture and mutants thereof bearing the ARS Culture Collection Accession No. NRRL B-15992.
Claim 11
Dependent Claim
18 words
An isolated and biologically pure culture and mutants thereof bearing the ARS Culture Collection Accession No. NRRL B-15992.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-08-31 | 1991-03-02 | 1991-09-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-08-31 | 1995-03-02 | 1995-09-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-08-31 | 1999-03-02 | 1999-09-01 | $7700.00 | Window Closed / Lapsed |