US4701411A
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Bilirubin-specific enzyme preparation, assay compositions, analytical elements and methods using same
Assignee: Eastman Kodak Co
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Filing: 1983-09-01
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Granted: 1987-10-20
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Expires: 2003-09-01
Abstract
Abstract An enzyme preparation having specific bilirubin degrading activity is described. The enzymes useful therein are obtained from various higher plant sources of the families Solanaceae, Musaceae and Liliaceae. Specific plant sources include eggplants, tomatoes and potatoes from the Solanaceae family, bananas from the Musaceae family, and onions from the Liliaceae family. Assay compositions, analytical elements and methods for use of such are also described.
Inventors
- 👤 Tai-Wing Wu
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 21:20
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12Q; C12Q1/00; C12Q1/34; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10T; Y10T436/00; Y10T436/14; Y10T436/145555; Y10T436/146666
Claims Analysis
Total Claims: 30
Claim 1
Independent Claim
65 words
A bilirubin-specific enzyme preparation comprising an enzyme isolated from a plant of the group of families consisting of Solanaceae, Musaceae and Liliaceae which, in the presence of a bilirubin-containing aqueous liquid, degrades bilirubin at a pH in the range of from about 6 to about 10 and a temperature in the range of from about 20° to about 50° C. without generation of hydrogen peroxide.
Claim 2
Dependent Claim
65 words
A bilirubin-specific enzyme preparation comprising an enzyme isolated from a plant of the group of families consisting of Solanaceae, Musaceae and Liliaceae which, in the presence of a bilirubin-containing aqueous liquid, degrades bilirubin at a pH in the range of from about 6 to about 10 and a temperature in the range of from about 20° to about 50° C. without generation of hydrogen peroxide.
Claim 3
Dependent Claim
24 words
The preparation of claim 1 wherein said enzyme is isolated from a plant of the Solanum, Capsicum or Lycopersicum genera of the family Solanaceae.
Claim 4
Dependent Claim
12 words
The preparation of claim 2 wherein said enzyme is isolated from eggplants.
Claim 5
Dependent Claim
21 words
The preparation of claim 1 wherein said enzyme is isolated from a plant of the Musa genus of the family Musaceae.
Claim 6
Dependent Claim
12 words
The preparation of claim 4 wherein said enzyme is isolated from bananas.
Claim 7
Dependent Claim
21 words
The preparation of claim 1 wherein said enzyme is isolated from a plant of the Allium genus of the family Liliaceae.
Claim 8
Dependent Claim
12 words
The preparation of claim 6 wherein said enzyme is isolated from onions.
Claim 9
Dependent Claim
108 words
An assay composition for the determination of an analyte other than bilirubin in an aqueous liquid sample in which bilirubin is an interferent for said determination, said composition comprising an interactive composition for said analyte and a bilirubin-specific enzyme preparation comprising an enzyme derived from a plant of the group of families consisting of Solanaceae, Musaceae and Liliaceae which, in the presence of a bilirubin-containing aqueous liquid, degrades bilirubin at a pH in the range of from about 6 to about 10 and a temperature in the range of from about 20° to about 50° C. without generation of hydrogen peroxide and reduces bilirubin interference with said assay.
Claim 10
Dependent Claim
108 words
An assay composition for the determination of an analyte other than bilirubin in an aqueous liquid sample in which bilirubin is an interferent for said determination, said composition comprising an interactive composition for said analyte and a bilirubin-specific enzyme preparation comprising an enzyme derived from a plant of the group of families consisting of Solanaceae, Musaceae and Liliaceae which, in the presence of a bilirubin-containing aqueous liquid, degrades bilirubin at a pH in the range of from about 6 to about 10 and a temperature in the range of from about 20° to about 50° C. without generation of hydrogen peroxide and reduces bilirubin interference with said assay.
Claim 11
Dependent Claim
9 words
The assay composition of claim 8 comprising a buffer.
Claim 12
Dependent Claim
77 words
An analytical element for detecting an analyte, said element comrpising a self-supporting reagent zone containing a bilirubin-specific enzyme isolated from a plant of the group of families consisting of Solanaceae, Musaceae and Liliaceae which, in the presence of a bilirubin-containing aqueous liquid, degrades bilirubin at a pH in the range of from about 6 to about 10 and a tempera- ture in the range of from about 20° to about 50° C. without generation of hydrogen peroxide.
Claim 13
Dependent Claim
77 words
An analytical element for detecting an analyte, said element comrpising a self-supporting reagent zone containing a bilirubin-specific enzyme isolated from a plant of the group of families consisting of Solanaceae, Musaceae and Liliaceae which, in the presence of a bilirubin-containing aqueous liquid, degrades bilirubin at a pH in the range of from about 6 to about 10 and a tempera- ture in the range of from about 20° to about 50° C. without generation of hydrogen peroxide.
Claim 14
Dependent Claim
82 words
A dry analytical element for the assay of an aqueous liquid, said element including a support and a reagent zone containing a bilirubin-specific enzyme derived from a plant of the group of families consisting of Solanaceae, Musaceae and Liliaceae which, in the presence of a bilirubin-containing aqueous liquid, degrades bilirubin at a pH in the range of from about 6 to about 10 and a temperature in the range of from about 20° to about 50° C. without generation of hydrogen peroxide.
Claim 15
Dependent Claim
82 words
A dry analytical element for the assay of an aqueous liquid, said element including a support and a reagent zone containing a bilirubin-specific enzyme derived from a plant of the group of families consisting of Solanaceae, Musaceae and Liliaceae which, in the presence of a bilirubin-containing aqueous liquid, degrades bilirubin at a pH in the range of from about 6 to about 10 and a temperature in the range of from about 20° to about 50° C. without generation of hydrogen peroxide.
Claim 16
Dependent Claim
23 words
The element of claim 11 wherein said enzyme is present in an amount of from about 40 to about 400 U/m 2 .
Claim 17
Dependent Claim
25 words
The dry element of claim 11 wherein said enzyme is derived from a plant of the Solanum, Capsicum or Lycopersicum genera of the family Solanaceae.
Claim 18
Dependent Claim
13 words
The dry element of claim 13 wherein said enzyme is derived from eggplants.
Claim 19
Independent Claim
91 words
A method for the degradation of bilirubin in an aqueous liquid sample, said method comprising contacting said liquid sample with a bilirubin-specific enzyme preparation to degrade bilirubin, said enzyme preparation containing an enzyme isolated from a plant of the group of families consisting of Solanaceae, Musaceae and Liliaceae which, in the presence of a bilirubin-containing aqueous liquid, degrades bilirubin at a pH in the range of from about 6 to about 10 and a temperature in the range of from about 20° to about 50° C. without generation of hydrogen peroxide.
Claim 20
Independent Claim
91 words
A method for the degradation of bilirubin in an aqueous liquid sample, said method comprising contacting said liquid sample with a bilirubin-specific enzyme preparation to degrade bilirubin, said enzyme preparation containing an enzyme isolated from a plant of the group of families consisting of Solanaceae, Musaceae and Liliaceae which, in the presence of a bilirubin-containing aqueous liquid, degrades bilirubin at a pH in the range of from about 6 to about 10 and a temperature in the range of from about 20° to about 50° C. without generation of hydrogen peroxide.
Claim 21
Dependent Claim
12 words
The method of claim 15 wherein said aqueous liquid is whole blood.
Claim 22
Independent Claim
110 words
A method for the determination of bilirubin in an aqueous liquid sample, said method comprising the steps of: (a) contacting said liquid sample with a bilirubin-specific enzyme preparation to interact bilirubin with said enzyme preparation and to produce a detectable change, said enzyme preparation containing an enzyme derived from a plant of the group of families consisting of Solanaceae, Musaceae and Liliaceae which, in the presence of a bilirubin-containing aqueous liquid, degrades bilirubin at a pH in the range of from about 6 to about 10 and a temperature in the range of from about 20° to about 50° C. without generation of hydrogen peroxide; and (b) detecting said change.
Claim 23
Independent Claim
110 words
A method for the determination of bilirubin in an aqueous liquid sample, said method comprising the steps of: (a) contacting said liquid sample with a bilirubin-specific enzyme preparation to interact bilirubin with said enzyme preparation and to produce a detectable change, said enzyme preparation containing an enzyme derived from a plant of the group of families consisting of Solanaceae, Musaceae and Liliaceae which, in the presence of a bilirubin-containing aqueous liquid, degrades bilirubin at a pH in the range of from about 6 to about 10 and a temperature in the range of from about 20° to about 50° C. without generation of hydrogen peroxide; and (b) detecting said change.
Claim 24
Independent Claim
122 words
A method for the determination of bilirubin in an aqueous liquid sample, said method comprising the steps of: (a) contacting said liquid sample with an analytical element containing a bilirubin-specific enzyme to interact bilirubin with said enzyme and to produce a detectable change, said element including a support and a reagent zone containing said bilirubin-specific enzyme which is derived from a plant of the group of families consisting of Solanaceae, Musaceae and Liliaceae and which, in the presence of a bilirubin-containing aqueous liquid, degrades bilirubin at a pH in the range of from about 6 to about 10 and a temperature in the range of from about 20° to about 50° C. without generation of hydrogen peroxide; and (b) detecting said change.
Claim 25
Independent Claim
122 words
A method for the determination of bilirubin in an aqueous liquid sample, said method comprising the steps of: (a) contacting said liquid sample with an analytical element containing a bilirubin-specific enzyme to interact bilirubin with said enzyme and to produce a detectable change, said element including a support and a reagent zone containing said bilirubin-specific enzyme which is derived from a plant of the group of families consisting of Solanaceae, Musaceae and Liliaceae and which, in the presence of a bilirubin-containing aqueous liquid, degrades bilirubin at a pH in the range of from about 6 to about 10 and a temperature in the range of from about 20° to about 50° C. without generation of hydrogen peroxide; and (b) detecting said change.
Claim 26
Dependent Claim
18 words
The method of claim 18 wherein said detectable change is a change in the absorption density of bilirubin.
Claim 27
Dependent Claim
24 words
The method of claim 18 wherein said enzyme is derived from a plant of the Solanum, Capsicum or Lycopersicum genera of the family Solanaceae.
Claim 28
Dependent Claim
12 words
The method of claim 20 wherein said enzyme is derived from eggplants.
Claim 29
Independent Claim
147 words
A method for the determination of an analyte other than bilirubin in an aqueous liquid sample in which bilirubin is an interferent for said determination, said method comprising the steps of: (a) treating said sample with an interactive composition for said analyte to produce a detectable change; (b) prior to or during step (a), contacting said sample with a bilirubin-specific enzyme preparation containing an enzyme derived from a plant of the group of families consisting of Solanaceae, Musaceae and Liliaceae which, in the presence of a bilirubin-containing aqueous liquid, degrades bilirubin at a pH in the range of from about 6 to about 10 and a temperature in the range of from about 20° to about 50° C. without generation of hydrogen peroxide, thereby reducing the potential of bilirubin interference with said detectable change produced in step (a); and (c) detecting said change produced in step (a).
Claim 30
Independent Claim
147 words
A method for the determination of an analyte other than bilirubin in an aqueous liquid sample in which bilirubin is an interferent for said determination, said method comprising the steps of: (a) treating said sample with an interactive composition for said analyte to produce a detectable change; (b) prior to or during step (a), contacting said sample with a bilirubin-specific enzyme preparation containing an enzyme derived from a plant of the group of families consisting of Solanaceae, Musaceae and Liliaceae which, in the presence of a bilirubin-containing aqueous liquid, degrades bilirubin at a pH in the range of from about 6 to about 10 and a temperature in the range of from about 20° to about 50° C. without generation of hydrogen peroxide, thereby reducing the potential of bilirubin interference with said detectable change produced in step (a); and (c) detecting said change produced in step (a).
References Cited (Backward Citations)
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