US4703003A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Monoclonal antibody with a high affinity for digoxin
Assignee: Roche Diagnostics GmbH
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Filing: 1984-08-13
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Granted: 1987-10-27
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Expires: 2004-08-13
Abstract
Abstract A hybridoma which produces monoclonal antibodies having a high affinity and selectivity for digoxin is produced by immunizing mice with digoxin, fusing the spleen cells from the treated mice with mice myeloma cells, separating hybrids from non-fused cells, selecting the hybrids which produce monoclonal antibodies directed against digoxin and isolating the hybrids.
Inventors
- 👤 Carl-Julius Struck
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 14:00
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/44; A; A61; A61P; A61P9/00; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
31 words
A hybridoma which produces monoclonal antibodies having a high affinity and selectivity for digoxin, a cross-reactivity of less than 1.3% with digitoxin and a cross-reactivity of less than 0.007% with spironolactone.
Claim 2
Dependent Claim
31 words
A hybridoma which produces monoclonal antibodies having a high affinity and selectivity for digoxin, a cross-reactivity of less than 1.3% with digitoxin and a cross-reactivity of less than 0.007% with spironolactone.
Claim 3
Dependent Claim
25 words
A hybridoma of claim 1 produced by immunizing mice with digoxin and carrying out selection of the hybrid cells with digoxin, digitalis glycosides and spironolactone.
Claim 4
Dependent Claim
15 words
A hybridoma of claim 2 which is produced by using digitoxin as the digitalis glycoside.
Claim 5
Dependent Claim
28 words
A monoclonal antibody having a high affinity and specificity for digoxin, a cross-reactivity of less than 1.3% with digitoxin and a cross-reactivity of less than 0.007% with spironalactone.
Claim 6
Dependent Claim
28 words
A monoclonal antibody having a high affinity and specificity for digoxin, a cross-reactivity of less than 1.3% with digitoxin and a cross-reactivity of less than 0.007% with spironalactone.
Claim 7
Dependent Claim
33 words
A monoclonal antibody of claim 4 which is prepared by immunizing mice with digoxin, carrying out the selection of the hybrid cells with digoxin, digitalis glycosides and spironolactone, and isolating the monoclonal antibody.
Claim 8
Dependent Claim
16 words
A monoclonal antibody of claim 5 which is produced by using digitoxin as the digitalis glycoside.
Claim 9
Dependent Claim
58 words
The method of producing a hybridoma of claim 1, which comprises (a) immunizing mice with digoxin, (b) fusing the spleen cells from the treated mice with mouse myeloma cells, (c) separating hybrids from non-fused cells, (d) selecting the hybrids which produce monoclonal antibodies directed against digoxin by using digoxin, digitalis glycosides and sprionolactone, and (e) isolating the hybrids.
Claim 10
Dependent Claim
13 words
The method of claim 7 where digitoxin is used as the digitalis glycoside.
Claim 11
Dependent Claim
70 words
The method of producing a monoclonal antibody of claim 4, which comprises (a) immunizing mice with digoxin, (b) fusing spleen cells from the treated mice with mouse myeloma cells, (c) separating hybrids from non-fused cells, (d) selecting the hybrids which produce monoclonal antibodies directed against digoxin by using digoxin digitalis glycosides and spironolactone, and (e) isolating the monoclonal antibodies after the growth of the cells in vivo or in vitro.
Claim 12
Dependent Claim
13 words
The method of claim 9, where digitoxin is used as the digitalis glycoside.
References Cited (Backward Citations)
US4477576A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-10-26 | 1991-04-27 | 1991-10-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-10-26 | 1995-04-27 | 1995-10-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-10-26 | 1999-04-27 | 1999-10-27 | $7700.00 | Window Closed / Lapsed |