US4707352A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of radioactively labeling diagnostic and therapeutic agents containing a chelating group
Assignee: Enzo Therapeutics Inc
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Filing: 1984-01-30
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Granted: 1987-11-17
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Expires: 2004-01-30
Abstract
Abstract A method of forming a therapeutic or diagnostic agent labeled with a radioactive metal ion, which comprises: contacting an unlabeled therapeutic or diagnostic agent, consisting of a substantially non-metal chelating portion and a chelating portion capable of chelating with the radioactive metal ion, with an ion transfer material having the radioactive metal ion bound thereto and having a binding affinity for the radioactive metal less than the binding affinity of the chelating portion for the radioactive metal ion, wherein prior to contacting the chelating portion is unchelated or is chelated with a second metal having a binding affinity with the chelating portion less than the binding affinity of the radioactive metal ion, whereby a radiolabeled therapeutic or diagnostic agent is formed by the contacting, and separating the radiolabeled therapeutic or diagnostic agent from the ion transfer material, is disclosed along with various components and kits useful in practicing this method and several variations thereof.
Inventors
- 👤 Jannis G. Stavrianopoulos
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 09:47
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/60; A; A61; A61K; A61K51/00; A61K51/02; A61K51/04; A61K51/0474; A61K51/0478; A61K51/12; G01N33/53; G01N33/531; G01N33/532; G01N33/534; A61K2121/00; A61K2123/00; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/808; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
158 words
A method of forming a therapeutic or diagnostic agent labeled with a radioactive metal ion, which comprises: (A) contacting (1) an unlabeled therapeutic or diagnostic agent comprising (a) a molecularly recognizable portion attached to (b) a chelating portion capable of substantially chelating with said radioactive metal ion, wherein said chelating portion is not a part of said molecularly recognizable portion, with (2) an ion transfer material having said radioactive metal ion bound thereto and having a binding affinity for said radioactive metal ion less than the binding affinity of said chelating portion for said radioactive metal ion, wherein, prior to said contacting, said chelating portion is unchelated or is chelated with a second metal having a binding affinity with said chelating portion less than the binding affinity of said radioactive metal ion, whereby a radiolabeled therapeutic or diagnostic agent is formed by said contacting; and (B) separating said radiolabeled therapeutic or diagnostic agent from said ion transfer material.
Claim 2
Independent Claim
158 words
A method of forming a therapeutic or diagnostic agent labeled with a radioactive metal ion, which comprises: (A) contacting (1) an unlabeled therapeutic or diagnostic agent comprising (a) a molecularly recognizable portion attached to (b) a chelating portion capable of substantially chelating with said radioactive metal ion, wherein said chelating portion is not a part of said molecularly recognizable portion, with (2) an ion transfer material having said radioactive metal ion bound thereto and having a binding affinity for said radioactive metal ion less than the binding affinity of said chelating portion for said radioactive metal ion, wherein, prior to said contacting, said chelating portion is unchelated or is chelated with a second metal having a binding affinity with said chelating portion less than the binding affinity of said radioactive metal ion, whereby a radiolabeled therapeutic or diagnostic agent is formed by said contacting; and (B) separating said radiolabeled therapeutic or diagnostic agent from said ion transfer material.
Claim 3
Dependent Claim
18 words
The method of claim 1, wherein the molecularly recognizable portion, is a natural constituent of a biological system.
Claim 4
Dependent Claim
15 words
The method of claim 1, wherein the molecularly recognizable portion of said agent is proteinaceous.
Claim 5
Dependent Claim
16 words
The method of claim 1, wherein the molecularly recognizable portion of said agent comprises nucleic acid.
Claim 6
Dependent Claim
16 words
The method of claim 1, wherein the molecularly recognizable portion on said agent comprises a saccharide.
Claim 7
Dependent Claim
16 words
The method of claim 1, wherein the moleculary recognizable portion of said agent comprises a hormone.
Claim 8
Dependent Claim
34 words
The method of claim 1, wherein the moleculary recognizable portion of said agent is selected from the group consisting of antigens, antibodies, hormone receptors, viruses, viral components, bacteria, bacterial compounds, cells and cellular components.
Claim 9
Dependent Claim
26 words
The method of claim 1, wherein the chelating portion comprises a diamine having four acetic acid moieties attached to the two amino groups of said diamine.
Claim 10
Dependent Claim
16 words
The method of claim 8, wherein the amino groups are covalently attached to adjacent carbon atoms.
Claim 11
Dependent Claim
20 words
The method of claim 9, wherein said chelating portion is a derivative or analog of ethylenediaminetetraacetic acid or trans-1,2-diaminocyclohexaneacetic acid.
Claim 12
Dependent Claim
41 words
The method of claim 10, wherein said chelating portion comprises a radical having the formula ##STR2## wherein R 1 is CH 2 --COOM or a C 1 -C 4 alkyl and M is hydrogen, a cationic metal or a negative charge.
Claim 13
Dependent Claim
39 words
The method of claim 1, wherein the fraction of the binding affinity of said ion transfer material for said radioactive metal ion compared to the binding affinity of said chelating portion for said radioactive ion is less than 0.1.
Claim 14
Dependent Claim
12 words
The method of claim 12, wherein said fraction is less than 0.01.
Claim 15
Dependent Claim
12 words
The method of claim 13, wherein said fraction is less than 0.001.
Claim 16
Dependent Claim
15 words
The method of claim 1, wherein said ion transfer material is a strong-acid cation-exchange resin.
Claim 17
Dependent Claim
19 words
The method of claim 15, wherein said resin is a sulfonated copolymer of styrene and a diene crosslinking agent.
Claim 18
Dependent Claim
11 words
The method of claim 16, wherein said cross-linking agent is divinylbenzene.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-11-16 | 1991-05-18 | 1991-11-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-11-16 | 1995-05-18 | 1995-11-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-11-16 | 1999-05-18 | 1999-11-17 | $7700.00 | Window Closed / Lapsed |