US4708818A
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Human immunodeficiency viruses associated with Acquired Immune Deficiency Syndrome (AIDS), a diagnostic method for AIDS and pre-AIDS, and a kit therefor
Assignee: Institut Pasteur
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Filing: 1985-10-08
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Granted: 1987-11-24
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Expires: 2005-10-08
Abstract
Abstract Retroviruses associated with Acquired Immune Deficiency Syndrome (AIDS), including Lymphadenopathy Associated Virus (LAV), are isolated from the sera of patients afflicted with Lymphadenopathy Syndrome (LAS) or AIDS. LAV is a Human Immunodeficiency Virus (HIV). Viral extract, structural proteins and other fractions of the retrovirus immunologically recognize the sera of such patients. Immunological reaction is used to detect antibodies that specifically bind to antigenic sites of the retrovirus in samples of body fluids from patients with AIDS or risk of AIDS. A kit for in vitro assay of LAS or AIDS is provided.
Inventors
- 👤 Luc Montagnier
- 👤 Jean-Claude Chermann
- 👤 Francoise Barre-Sinoussi
- 👤 Francoise Brun-Vezinet
- 👤 Christine Rouzioux
- 👤 Willy Rozenbaum
- 👤 Charles Dauguet
- 👤 Jacqueline Gruest
- 👤 Marie-Therese Nugeyre
- 👤 Francoise Rey
- 👤 Claudine Axler-Blin
- 👤 Solange Chamaret
- 👤 Robert C. Gallo
- 👤 Mikulas Popovic
- 👤 Mangalasseril G. Sarngadharan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 16:34
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C12; C12Q; C12Q1/00; A; A61; A61P; A61P31/00; A61P31/12; A61P31/14; A61P31/18; C12N; C12N7/00; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56983; G01N33/56988; C12N2740/00; C12N2740/00011; C12N2740/10011; C12N2740/16011; C12N2740/16021; C12N2740/16051; C12N2740/16211; C12N2740/16222; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/8215; Y10S435/948; Y10S435/974; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/808; Y10S436/811
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
85 words
An in vitro diagnostic method for the detection of the presence or absence of antibodies which bind to antigens of a human retrovirus indicative of acquired immune deficiency syndrome (AIDS) or of lymphadenopathy-associated syndrome (pre-AIDS), which method comprises contacting a lysate of said human retrovirus comprising antigens of said retrovirus with a biological fluid for a time and under conditions sufficient for the retroviral antigens in the lysate and antibodies in the biological fluid to form antigen-antibody complexes and detecting the formation of the complexes.
Claim 2
Dependent Claim
85 words
An in vitro diagnostic method for the detection of the presence or absence of antibodies which bind to antigens of a human retrovirus indicative of acquired immune deficiency syndrome (AIDS) or of lymphadenopathy-associated syndrome (pre-AIDS), which method comprises contacting a lysate of said human retrovirus comprising antigens of said retrovirus with a biological fluid for a time and under conditions sufficient for the retroviral antigens in the lysate and antibodies in the biological fluid to form antigen-antibody complexes and detecting the formation of the complexes.
Claim 3
Dependent Claim
18 words
The method of claim 1 wherein the detecting step further comprises measuring the formation of said antigen-antibody complexes.
Claim 4
Dependent Claim
12 words
The method of claim 1 wherein the biological fluid is human sera.
Claim 5
Dependent Claim
15 words
The method of claim 1 wherein the biological fluid is from a patient with pre-AIDS.
Claim 6
Dependent Claim
28 words
The method of claim 1 wherein the human retrovirus is selected from the group consisting of a lymphadenopathy associated virus (LAV) and an immune deficiency associated virus (IDAV).
Claim 7
Dependent Claim
11 words
The method of claim 5 wherein the human retrovirus is LAV.
Claim 8
Dependent Claim
11 words
The method of claim 6 wherein the human retrovirus is LAV1.
Claim 9
Dependent Claim
18 words
The method of claim 5 wherein the human retrovirus is selected from the group of IDAV1 and IDAV2.
Claim 10
Dependent Claim
76 words
A diagnostic kit for the detection of the presence or absence of antibodies which bind to antigens of a human retrovirus indicative of acquired immune deficiency syndrome (AIDS) or of lymphadenopathy-associated syndrome (pre-AIDS), which kit comprises a retroviral lysate which comprises antigens of said retrovirus and means for detecting the formation of immune complexes between said antigens and said antibodies, wherein the lysate and the means are present in an amount sufficient to perform said detection.
Claim 11
Dependent Claim
76 words
A diagnostic kit for the detection of the presence or absence of antibodies which bind to antigens of a human retrovirus indicative of acquired immune deficiency syndrome (AIDS) or of lymphadenopathy-associated syndrome (pre-AIDS), which kit comprises a retroviral lysate which comprises antigens of said retrovirus and means for detecting the formation of immune complexes between said antigens and said antibodies, wherein the lysate and the means are present in an amount sufficient to perform said detection.
Claim 12
Dependent Claim
34 words
The diagnostic kit of claim 9 wherein the means for detecting the formation of the immune complexes are immunological assay means selected from the group consisting of radioimmuno assay, immuno-enzymatic and immunofluorescent assay means.
Claim 13
Independent Claim
99 words
A method for detection of antibodies which specifically bind to antigenic sites of the AIDS or pre-AIDS-associated LAV virus in samples of body fluids of patients with Acquired Immune Deficiency Syndrome (AIDS) or risk of AIDS (pre-AIDS) which comprises contacting virus fractions or a virus extract or a lysate of said virus with antibodies from human sera for a time and under conditions sufficient to permit formation of antigen-antibody complexes between said virus fractions, virus extract or lysate and said antibodies and measuring the formation of said antigen-antibody complexes by ELISA (an enzyme-linked immunosorbent assay) or indirect immunofluorescent assay.
Claim 14
Independent Claim
99 words
A method for detection of antibodies which specifically bind to antigenic sites of the AIDS or pre-AIDS-associated LAV virus in samples of body fluids of patients with Acquired Immune Deficiency Syndrome (AIDS) or risk of AIDS (pre-AIDS) which comprises contacting virus fractions or a virus extract or a lysate of said virus with antibodies from human sera for a time and under conditions sufficient to permit formation of antigen-antibody complexes between said virus fractions, virus extract or lysate and said antibodies and measuring the formation of said antigen-antibody complexes by ELISA (an enzyme-linked immunosorbent assay) or indirect immunofluorescent assay.
Claim 15
Independent Claim
88 words
A method for detection of antibodies which specifically bind to antigenic sites of the AIDS or pre-AIDS-associated LAV virus in samples of body fluids of patients with Acquired Immune Deficiency Syndrome (AIDS) or risk of AIDS (pre-AIDS) which comprises contacting virus fractions or a virus extract or a lysate of said virus with antibodies from human sera for a time and under conditions sufficient to permit formation of antigen-antibody complexes between said virus fractions, virus extract or lysate and said antibodies and detecting the formation of said complexes.
Claim 16
Independent Claim
88 words
A method for detection of antibodies which specifically bind to antigenic sites of the AIDS or pre-AIDS-associated LAV virus in samples of body fluids of patients with Acquired Immune Deficiency Syndrome (AIDS) or risk of AIDS (pre-AIDS) which comprises contacting virus fractions or a virus extract or a lysate of said virus with antibodies from human sera for a time and under conditions sufficient to permit formation of antigen-antibody complexes between said virus fractions, virus extract or lysate and said antibodies and detecting the formation of said complexes.
References Cited (Backward Citations)
US4323555A
Cited
US4520113A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-11-23 | 1991-05-25 | 1991-11-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-11-23 | 1995-05-25 | 1995-11-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-11-23 | 1999-05-25 | 1999-11-24 | $7700.00 | Window Closed / Lapsed |