US4732849A
Expired / Lapsed
US
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Multilayered chemical analysis material for analysis of aqueous liquids
Assignee: Fujifilm Holdings Corp
•
Filing: 1986-02-10
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Granted: 1988-03-22
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Expires: 2006-02-10
Abstract
Abstract A multi-layered, chemical analysis material for analysis of an aqueous liquid, which comprises in one embodiment (a) a light-transmitting, water-impermeable support having provided thereon in sequence (b) a reagent layer and (c) a porous, sample-spreading layer, with (i) the reagent layer (b) containing a lake dye precursor dispersed in a hydrophilic binder, (ii) urease incorporated in the reagent layer (b) or in a layer adjacent the reagent layer (b), and (iii) a lake forming metal salt or lake forming metal oxide, which does not substantially inhibit the activity of urease, incorporated in the reagent layer (b) or in a layer adjacent the reagent layer (b) or a lake forming metal salt or oxide incorporated in a layer adjacent the reagent layer (b); and in a second embodiment the chemical analysis material includes (d) a radiation-blocking layer interposed between said reagent layer (b) and said porous, sample-spreading layer (c).
Inventors
- 👤 Osamu Seshimoto
- 👤 Masao Kitajima
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 17:12
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525; C; C12; C12Q; C12Q1/00; C12Q1/58; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 58
Claim 1
Independent Claim
153 words
A multi-layered, chemical analysis element for analysis of an aqueous liquid, which comprises: (a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a reagent layer; and (c) a porous, sample-spreading layer; with (i) said reagent layer (b) containing a lake dye precursor dispersed in a hydrophilic biner, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, (ii) urease incorporated in the reagent layer (b) or in a layer immediately adjacent the reagent layer (b), and (iii) a member selected from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt, incorporated in at least one of the reagent layer (b) and a layer immediately adjacent the reagent layer (b).
Claim 2
Dependent Claim
153 words
A multi-layered, chemical analysis element for analysis of an aqueous liquid, which comprises: (a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a reagent layer; and (c) a porous, sample-spreading layer; with (i) said reagent layer (b) containing a lake dye precursor dispersed in a hydrophilic biner, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, (ii) urease incorporated in the reagent layer (b) or in a layer immediately adjacent the reagent layer (b), and (iii) a member selected from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt, incorporated in at least one of the reagent layer (b) and a layer immediately adjacent the reagent layer (b).
Claim 3
Dependent Claim
26 words
The chemical analysis element of claim 1, wherein said light-transmitting, water-impermeable support is a synthetic resin film, a film of regenerated cellulose or a glass plate.
Claim 4
Dependent Claim
27 words
The chemical analysis element of claim 1, wherein said porous, sample-spreading layer is a layer of a fabric comprised of natural fibers, synthetic fibers or mixtures thereof.
Claim 5
Dependent Claim
19 words
The chemical analysis element of claim 3, wherein said fabric is one subjected to a treatment rendering such hydrophilic.
Claim 6
Dependent Claim
21 words
The chemical analysis element of claim 1, wherein said porous, sample-spreading layer is a layer of a non-fibrous, isotropic, porous material.
Claim 7
Dependent Claim
14 words
The chemical analysis element of claim 1, wherein said lake dye precursor is haematoxylin.
Claim 8
Dependent Claim
29 words
The chemical analysis element of claim 1, wherein said element is such that color reaction in said chemical analysis element occurs at a pH of about 6.5 or higher.
Claim 9
Dependent Claim
18 words
The chemical analysis element of claim 1, wherein said hydrophilic binder is a water-soluble high molecular weight polymer.
Claim 10
Dependent Claim
24 words
The chemical analysis element of claim 8, wherein said high molecular weight polymer is a water-soluble protein, a vegetable gum or a synthetic polymer.
Claim 11
Dependent Claim
29 words
The chemical analysis element of claim 1, wherein said element additionally contains in said reagent layer or another layer thereof at least one of a stabilizer and a buffer.
Claim 12
Dependent Claim
36 words
The chemical analysis element of claim 1, wherein at least one of urease, said lake forming metal salt or said lake forming metal oxide is incorporated in a layer directly adjacent to the reagent layer (b).
Claim 13
Dependent Claim
23 words
The chemical analysis element of claim 1, wherein said EDTA salt is selected from Fe(III) salt of EDTA and Na salt of EDTA.
Claim 14
Dependent Claim
31 words
The chemical analysis element of claim 1, wherein said lake forming metal salt or said lake forming metal oxide are present in the layer which is adjacent said reagent layer (b).
Claim 15
Dependent Claim
21 words
The chemical analysis element of claim 1, wherein said reagent layer (b) and said porous, sample-spreading layer (c) are hydrophilic layers.
Claim 16
Dependent Claim
17 words
The chemical analysis element of claim 1, wherein said porous, sample spreading layer contains a radiation-blocking powder.
Claim 17
Dependent Claim
157 words
A multi-layered, chemical analysis element for analysis of an aqueous liquid, which comprises: (a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a reagent layer; (c) a radiation-blocking layer; and (d) a porous, sample spreading layer; (i) said reagent layer (b) containing a lake dye precursor dispersed in a hydrophilic binder, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, (ii) urease incorporated in the reagent layer (b) or in a layer immediately adjacent the reagent layer (b), and (iii) a member selected from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt, incorporated in at least one of the reagent layer (b) and a layer immediately adjacent the reagent layer (b).
Claim 18
Dependent Claim
157 words
A multi-layered, chemical analysis element for analysis of an aqueous liquid, which comprises: (a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a reagent layer; (c) a radiation-blocking layer; and (d) a porous, sample spreading layer; (i) said reagent layer (b) containing a lake dye precursor dispersed in a hydrophilic binder, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, (ii) urease incorporated in the reagent layer (b) or in a layer immediately adjacent the reagent layer (b), and (iii) a member selected from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt, incorporated in at least one of the reagent layer (b) and a layer immediately adjacent the reagent layer (b).
Claim 19
Dependent Claim
26 words
The chemical analysis element of claim 16, wherein said light-transmitting, water-impermeable support is a synthetic resin film, a film of regenerated cellulose or a glass plate.
Claim 20
Dependent Claim
27 words
The chemical analysis element of claim 16, wherein said porous, sample-spreading layer is a layer of a fabric comprised of natural fibers, synthetic fibers or mixtures thereof.
Claim 21
Dependent Claim
19 words
The chemical analysis element of claim 18, wherein said fabric is one subjected to a treatment rendering such hydrophilic.
Claim 22
Dependent Claim
21 words
The chemical analysis element of claim 16, wherein said porous, sample-spreading layer is a layer of a non-fibrous, isotropic, porous material.
Claim 23
Dependent Claim
14 words
The chemical analysis element of claim 16, wherein said lake dye precursor is haematoxylin.
Claim 24
Dependent Claim
29 words
The chemical analysis element of claim 16, wherein said element is such that color reaction in said chemical analysis material occurs at a pH of about 6.5 or higher.
Claim 25
Dependent Claim
18 words
The chemical analysis element of claim 16, wherein said hydrophilic binder is a water-soluble high molecular weight polymer.
Claim 26
Dependent Claim
24 words
The chemical analysis element of claim 23, wherein said high molecular weight polymer is a water-soluble protein, a vegetable gum or a synthetic polymer.
Claim 27
Dependent Claim
29 words
The chemical analysis element of claim 16, wherein said element additionally contains in said reagent layer or another layer thereof at least one of a stabilizer and a buffer.
Claim 28
Dependent Claim
47 words
The chemical analysis element of claim 16, wherein said element comprises, in order from said light-transmitting, water-impermeable support, said reagent layer containing a lake dye precursor, urease, a lake forming metal salt or oxide and a buffering agent, said radiation blocking layer and said porous, sample-spreading layer.
Claim 29
Dependent Claim
36 words
The chemical analysis element of claim 16, wherein at least one of urease, said lake forming metal salt or said lake forming metal oxide is incorporated in a layer directly adjacent to the reagent layer (b).
Claim 30
Dependent Claim
23 words
The chemical analysis element of claim 16, wherein said EDTA salt is selected from Fe(III) salt of EDTA and Na salt of EDTA.
Claim 31
Dependent Claim
31 words
The chemical analysis element of claim 16, wherein said lake forming metal salt or said lake forming metal oxide are present in the layer which is adjacent said reagent layer (b).
Claim 32
Dependent Claim
143 words
A multi-layered, chemical analysis element for analysis of an aqueous liquid, which comprises: (a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a layer of a member selected from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt; (c) a reagent layer; and (d) a radiation blocking layer; and (e) a porous, sample-spreading layer; with (i) said reagent layer (c) containing a lake dye precursor dispersed in a hydrophilic binder, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, and (ii) urease incoporated in the reagent layer (c) or in a layer immediately adjacent the reagent layer (c).
Claim 33
Dependent Claim
143 words
A multi-layered, chemical analysis element for analysis of an aqueous liquid, which comprises: (a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a layer of a member selected from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt; (c) a reagent layer; and (d) a radiation blocking layer; and (e) a porous, sample-spreading layer; with (i) said reagent layer (c) containing a lake dye precursor dispersed in a hydrophilic binder, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, and (ii) urease incoporated in the reagent layer (c) or in a layer immediately adjacent the reagent layer (c).
Claim 34
Dependent Claim
137 words
A multi-layered, chemical anaylsis element for analysis of an aqueous liquid, which comprises (a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a layer of a member selected from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt; (c) a reagent layer; and (d) a porous, sample-spreading layer; with (i) said reagent layer (c) containing a lake dye precursor dispersed in a hydrophilic binder, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, and (ii) urease incorporated in the reagent layer (c) or in a layer immediately adjacent the reagent layer (c).
Claim 35
Dependent Claim
137 words
A multi-layered, chemical anaylsis element for analysis of an aqueous liquid, which comprises (a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a layer of a member selected from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt; (c) a reagent layer; and (d) a porous, sample-spreading layer; with (i) said reagent layer (c) containing a lake dye precursor dispersed in a hydrophilic binder, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, and (ii) urease incorporated in the reagent layer (c) or in a layer immediately adjacent the reagent layer (c).
Claim 36
Dependent Claim
17 words
The chemical analysis element of claim 31, wherein said porous, sample spreading layer contains a radiation-blocking powder.
Claim 37
Dependent Claim
157 words
A multi-layered, chemical analysis element for analysis of an aqueous liquid, which comprises (a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a reagent layer; (c) an adhesive layer; and (d) a porous, sample-spreading layer; with (i) said reagent layer (b) containing a lake dye precursor dispersed in a hydrophilic binder, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, (ii) urease incoporated in the reagent layer (b) or in a layer immediately adjacent the reagent layer (b), and (iii) a member selected from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt, incorporated in at least one of the reagent layer (b) and a layer immediately adjacent the reagent layer (b).
Claim 38
Dependent Claim
157 words
A multi-layered, chemical analysis element for analysis of an aqueous liquid, which comprises (a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a reagent layer; (c) an adhesive layer; and (d) a porous, sample-spreading layer; with (i) said reagent layer (b) containing a lake dye precursor dispersed in a hydrophilic binder, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, (ii) urease incoporated in the reagent layer (b) or in a layer immediately adjacent the reagent layer (b), and (iii) a member selected from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt, incorporated in at least one of the reagent layer (b) and a layer immediately adjacent the reagent layer (b).
Claim 39
Dependent Claim
13 words
A multi-layered, chemical analysis element for analysis of an aqueous liquid, which comprises:
Claim 40
Dependent Claim
13 words
A multi-layered, chemical analysis element for analysis of an aqueous liquid, which comprises:
Claim 41
Dependent Claim
148 words
(a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a reagent layer; (c) a radiation-blocking layer, (d) an adhesive layer; and (c) a porous, sample-spreading layer; with (i) said reagent layer (b) containing a lake dye precursor dispersed in a hydrophilic binder, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, (ii) urease incorporated in the reagent layer (b) or in a layer immediately adjacent the reagent layer (b), and (iii) a member selected from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt, incorporated in at least one of the reagent layer (b) and a layer immediately adjacent the reagent layer (b).
Claim 42
Dependent Claim
148 words
(a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a reagent layer; (c) a radiation-blocking layer, (d) an adhesive layer; and (c) a porous, sample-spreading layer; with (i) said reagent layer (b) containing a lake dye precursor dispersed in a hydrophilic binder, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, (ii) urease incorporated in the reagent layer (b) or in a layer immediately adjacent the reagent layer (b), and (iii) a member selected from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt, incorporated in at least one of the reagent layer (b) and a layer immediately adjacent the reagent layer (b).
Claim 43
Dependent Claim
141 words
A multi-layered, chemical analysis element for analysis of an aqueous liquid, which comprises: (a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a reagent layer; (c) a layer of a member selected from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt; (d) a radiation-blocking layer; and (e) a porous, sample-spreading layer; with (i) said reagent layer (b) containing a lake dye precursor dispersed in a hydrophilic binder, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, and (ii) urease incorporated in the reagent layer (b) or in a layer immediately adjacent the reagent layer (b).
Claim 44
Dependent Claim
141 words
A multi-layered, chemical analysis element for analysis of an aqueous liquid, which comprises: (a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a reagent layer; (c) a layer of a member selected from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt; (d) a radiation-blocking layer; and (e) a porous, sample-spreading layer; with (i) said reagent layer (b) containing a lake dye precursor dispersed in a hydrophilic binder, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, and (ii) urease incorporated in the reagent layer (b) or in a layer immediately adjacent the reagent layer (b).
Claim 45
Dependent Claim
17 words
The chemical analysis element of claim 33, wherein said porous, sample spreading layer contains a radiation-blocking powder.
Claim 46
Dependent Claim
137 words
A multi-layered, chemical analysis element for analysis of an aqueous liquid, which comprises: (a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a reagent layer; (c) a layer of a member selected from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt; and (d) a porous, sample-spreading layer; with (i) said reagent layer (b) containing a lake dye precursor dispersed in a hydrophilic binder, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, and (ii) urease incorporated in the reagent layer (b) or in a layer immediately adjacent the reagent layer (b).
Claim 47
Dependent Claim
137 words
A multi-layered, chemical analysis element for analysis of an aqueous liquid, which comprises: (a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a reagent layer; (c) a layer of a member selected from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt; and (d) a porous, sample-spreading layer; with (i) said reagent layer (b) containing a lake dye precursor dispersed in a hydrophilic binder, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, and (ii) urease incorporated in the reagent layer (b) or in a layer immediately adjacent the reagent layer (b).
Claim 48
Dependent Claim
17 words
The chemical analysis element of claim 37, wherein said porous, sample spreading layer contains a radiation-blocking powder.
Claim 49
Dependent Claim
141 words
A multi-layered, chemical analysis element for analysis of an aqueous liquid, which comprises: (a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a reagent layer; (c) a layer of a member selected from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt; (d) an adhesive layer; and (e) a porous, sample-spreading layer; with (i) said reagent layer (b) containing a lake dye precursor dispersed in a hydrophilic binder, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, and (ii) urease incorporated in the reagent layer (b) or in a layer immediately adjacent the reagent layer (b).
Claim 50
Dependent Claim
141 words
A multi-layered, chemical analysis element for analysis of an aqueous liquid, which comprises: (a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a reagent layer; (c) a layer of a member selected from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt; (d) an adhesive layer; and (e) a porous, sample-spreading layer; with (i) said reagent layer (b) containing a lake dye precursor dispersed in a hydrophilic binder, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, and (ii) urease incorporated in the reagent layer (b) or in a layer immediately adjacent the reagent layer (b).
Claim 51
Dependent Claim
17 words
The chemical analysis element of claim 39, wherein said porous, sample spreading layer contains a radiation-blocking powder.
Claim 52
Dependent Claim
141 words
A multi-layered, chemical analysis element for analysis of an aqueous liquid, which comprises (a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a layer of a member selected from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt; (c) a reagent layer; (d) an adhesive layer; and (e) a porous, sample-spreading layer; with (i) said reagent layer (c) containing a lake dye precursor dispersed in a hydrophilic binder, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, and (ii) urease incorporated in the reagent layer (c) or in a layer immediately adjacent the reagent layer (c).
Claim 53
Dependent Claim
141 words
A multi-layered, chemical analysis element for analysis of an aqueous liquid, which comprises (a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a layer of a member selected from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt; (c) a reagent layer; (d) an adhesive layer; and (e) a porous, sample-spreading layer; with (i) said reagent layer (c) containing a lake dye precursor dispersed in a hydrophilic binder, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, and (ii) urease incorporated in the reagent layer (c) or in a layer immediately adjacent the reagent layer (c).
Claim 54
Dependent Claim
17 words
The chemical analysis element of claim 41, wherein said porous, sample spreading layer contains a radiation-blocking powder.
Claim 55
Dependent Claim
144 words
A multi-layered, chemical analysis element for analysis of an aqueous liquid, which comprises: (a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a reagent layer; (c) a layer of a member selecteed from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt; (d) a radiation-blocking layer; (e) an adhesive layer; and (f) a porous, sample-spreading layer; with (i) said reagent layer (b) containing a lake dye precursor dispersed in a hydrophilic binder, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, (ii) urease incorporated in the reagent layer (b) or in a layer immediately adjacent the reagent layer (b).
Claim 56
Dependent Claim
144 words
A multi-layered, chemical analysis element for analysis of an aqueous liquid, which comprises: (a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a reagent layer; (c) a layer of a member selecteed from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt; (d) a radiation-blocking layer; (e) an adhesive layer; and (f) a porous, sample-spreading layer; with (i) said reagent layer (b) containing a lake dye precursor dispersed in a hydrophilic binder, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, (ii) urease incorporated in the reagent layer (b) or in a layer immediately adjacent the reagent layer (b).
Claim 57
Dependent Claim
145 words
A multi-layered, chemical analysis element for analysis of an aqueous liquid, which comprises: (a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a layer of a member selected from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt; (c) a reagent layer; (d) a radiation-blocking layer; (e) an adhesive layer; and (f) a porous, sample-spreading layer; with (i) said reagent layer (c) containing a lake dye precursor dispersed in a hydrophilic binder, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, and (ii) urease incorporated in the reagent layer (c) or in a layer immediately adjacent the reagent layer (c).
Claim 58
Dependent Claim
145 words
A multi-layered, chemical analysis element for analysis of an aqueous liquid, which comprises: (a) a light-transmitting, water impermeable support having provided thereon in sequence and without any intermediate layer; (b) a layer of a member selected from the group consisting of a lake forming metal salt and a lake forming metal oxide selected from the group consisting of TiO 2 , SiO 2 , Al 2 O 3 and an EDTA metal salt; (c) a reagent layer; (d) a radiation-blocking layer; (e) an adhesive layer; and (f) a porous, sample-spreading layer; with (i) said reagent layer (c) containing a lake dye precursor dispersed in a hydrophilic binder, wherein said lake dye precursor is haematoxylin, brazilin, dithizone, diphenylcarbazide, oxine, rubeanic acid, formaldoxime, dimethylglyoxime, alizarin, or sodium rhodinate, and (ii) urease incorporated in the reagent layer (c) or in a layer immediately adjacent the reagent layer (c).
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1991-03-22 | 1991-09-21 | 1992-03-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-03-22 | 1995-09-21 | 1996-03-22 | $3760.00 | Window Closed / Lapsed |
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