US4736018A
Expired / Lapsed
US
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Blood coagulation inhibiting proteins, processes for preparing them and their uses
Assignee: Boehringer Ingelheim International GmbH
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Filing: 1985-09-23
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Granted: 1988-04-05
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Expires: 2005-09-23
Abstract
Abstract This invention discloses proteins which inhibit the coagulation of the blood, processes for preparing these proteins, and the use thereof.
Inventors
- 👤 Christian P. M. Reutelingsperger
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 15:34
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/14; A; A61; A61K; A61K35/00; A61K35/12; A61K35/44; A61P; A61P7/00; A61P7/02; C07K1/16; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
75 words
A process for preparing an anti-coagulant protein from tissue which comprises: (a) homogenizing said tissue, differentially centrifuging said homogenized tissue, and subjecting the supernatant liquid to one or more of the following purification treatments in any desired sequence: (b) precipitation with salt, (c) affinity chromatography, (d) ion exchange chromatography, (e) chromatography using a molecular sieve; wherein said tissue is selected from the group consisting of blood vessel walls, highly vascularized tissue, or endothelial cell cultures.
Claim 2
Dependent Claim
75 words
A process for preparing an anti-coagulant protein from tissue which comprises: (a) homogenizing said tissue, differentially centrifuging said homogenized tissue, and subjecting the supernatant liquid to one or more of the following purification treatments in any desired sequence: (b) precipitation with salt, (c) affinity chromatography, (d) ion exchange chromatography, (e) chromatography using a molecular sieve; wherein said tissue is selected from the group consisting of blood vessel walls, highly vascularized tissue, or endothelial cell cultures.
Claim 3
Dependent Claim
15 words
The process of claim 1 wherein said anti-coagulant protein is further purified using immunoabsorption chromatography.
Claim 4
Dependent Claim
14 words
The process of claim 1 wherein said anti-coagulant protein is purified using phospholipid vesicles.
Claim 5
Dependent Claim
44 words
The process of claim 1 wherein ammonium sulfate is used for the precipitation in step (b), hydroxyapatite is used for chromatography in step (c), DEAE-Sephacel is used for chromatography in step (d), and Sephadex G-100 or G-75 is used for chromatography in step (e).
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-04-05 | 1991-10-05 | 1992-04-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-04-05 | 1995-10-05 | 1996-04-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-04-05 | 1999-10-05 | 2000-04-05 | $7700.00 | Window Closed / Lapsed |