US4736023A
Expired / Lapsed
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DNA encoding human CGRP
Assignee: Salk Institute for Biological Studies
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Filing: 1985-03-04
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Granted: 1988-04-05
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Expires: 2005-03-04
Abstract
Abstract Human CGRP (calcitonin-gene-related peptide) has the formula: H-Ala-Cys-Asn-Thr-Ala-Thr-Cys-Val-Thr-His-Arg-Leu-Ala-Gly-Leu-Leu-Ser-Arg-Ser-Gly-Gly-Val-Val-Lys-Asn-Asn-Phe-Val-Pro-Thr-Asn-Val-Gly-Ser-Lys-Ala-Phe-NH 2 . Human CGRP or pharmaceutically acceptable salts thereof, dispersed in a pharmaceutically acceptable liquid or solid carrier, can be administered to mammals, including humans, to influence memory, mood and pain appreciation and to achieve a substantial lowering of blood pressure or gastric acid secretion over an extended period of time. They also may be administered to affect ingestion behavior, taste and sensory perception. By using human CGRP to generate production of antibodies, it should be possible to diagnose human medullary thyroid carcinoma via immunoassay techniques.
Inventors
- 👤 Ronald M. Evans
- 👤 Michael G. Rosenfeld
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 23:16
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/57527; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
103 words
DNA encoding a precursor of human CGRP which includes a 37-codon nucleotide sequence that encodes a 37-amino acid residue CGRP peptide sequence, connected to DNA including at least the sequence CGG-CGG-AAG-AGA coding for the peptide sequence Arg-Arg-Arg-Arg-linked to the amino-terminus of said CGRP sequence peptide, and connected to DNA including at least the sequence GGC-CGC or GGC-AGG coding for a peptide segment including Gly-Arg linked to the carboxyl-terminus of said CGRP peptide sequence, said terminal peptide segments including signal segments for directing their enzymatic removal from said CGRP peptide sequence and for directing enzymatic amidation of the carboxyl-terminus of said CGRP peptide sequence.
Claim 2
Dependent Claim
103 words
DNA encoding a precursor of human CGRP which includes a 37-codon nucleotide sequence that encodes a 37-amino acid residue CGRP peptide sequence, connected to DNA including at least the sequence CGG-CGG-AAG-AGA coding for the peptide sequence Arg-Arg-Arg-Arg-linked to the amino-terminus of said CGRP sequence peptide, and connected to DNA including at least the sequence GGC-CGC or GGC-AGG coding for a peptide segment including Gly-Arg linked to the carboxyl-terminus of said CGRP peptide sequence, said terminal peptide segments including signal segments for directing their enzymatic removal from said CGRP peptide sequence and for directing enzymatic amidation of the carboxyl-terminus of said CGRP peptide sequence.
Claim 3
Dependent Claim
17 words
DNA according to claim 1 wherein said 37-codon nucleotide sequence encodes for the amino acid sequence: Ala-Cys-Asp-Thr-Ala-Thr-Cys-Val-Thr-His-Arg-Leu-Ala-Gly-Leu-Leu-Ser-Arg-Ser-Gly-Gly-Val-Val-Lys-Asn-Asn-Phe-Val-Pro-Thr-Asn-Val-Gly-Ser-Lys-Ala-Phe.
Claim 4
Dependent Claim
21 words
DNA according to claim 2 wherein said 37-codon nucleotide sequence is as follows: GCC-TGX-GAC-ACT-GCC-ACC-TGX-GTG-ACX-CAT-CGG-CTG-GCG-GGC-TTG-CTG-AGC-AGA-TCA-GGG-GGT-GTG-GTG-AAG-AAC-AAC-TTT-GTG-CCC-ACC-AAT-GTG-GGX-TCX-AAA -GCC-TTC, wherein Xis either I or C.
Claim 5
Dependent Claim
15 words
DNA according to claim 2 wherein said 37-codon nucleotide sequence is as follows: GCC-TGT-GAC-ACT-GCC-ACC-TGT-GTG-ACT-CAT-CGG-CTG-GCG-GGC-TTG-CTG-AGC-AGA-TCA-GGG-GGT-GTG-GTG-AAG-AAC-AAC-TTT-GTG-CCC-ACC-AAT-GTG-GGT-TCC-AAA -GCC-TTC.
Claim 6
Dependent Claim
15 words
DNA according to claim 1 wherein said 37-codon nucleotide sequence is as follows: GCC-TGC-AAC-ACT-GCC-ACC-TGC-GTG-ACC-CAT-CGG-CTG-GCG-GGC-TTG-CTG-AGC-AGA-TCA-GGG-GGT-GTG-GTG-AAG-AAC-AAC-TTT-GTG-CCC-ACC-AAT-GTG-GGC-TCT-AAA -GCC-CTC.
Claim 7
Dependent Claim
15 words
DNA according to claim 1 wherein said 37-codon nucleotide sequence is as follows: GCC-TGC-GAC-ACT-GCC-ACC-TGC-GTG-ACC-CAT-CGG-CTG-GCG-GGC-TTG-CTG-AGC-AGA-TCA-GGG-GGT-GTG-GTG-AAG-AAC-AAC-TTT-GTG-CCC-ACC-AAT-GTG-GGC-TCT-AAA -GCC-CTC.
Claim 8
Dependent Claim
15 words
DNA according to claim 1 wherein said 37-codon nucleotide sequence is as follows: GCC-TGC-AAC-ACT-GCC-ACC-TGC-GTG-ACC-CAT-CGG-CTG-GCG-GGC-TTG-CTG-AGC-AGA-TCA-GGG-GGT-GTG-GTG-AAG-AAC-AAC-TTT-GTG-CCC-ACC-AAT-GTG-GGC-TCT-AAA -GCC-TTC.
Claim 9
Dependent Claim
39 words
A DNA sequence including 37 codons that encode a 37-amino acid residue CGRP peptide sequence, wherein said 37-codon nucleotide sequence is as follows: GCC-TGX-YZC-ACT-GCC-ACC-TGC-GTG-ACC-CAT-CGG-CTG-GCG-GGC-TTG-CTG-AGC-AGA-TCA-GGG-GGT-GTG-GTG-AAG-AAC-AAC-TTT-GTG-CCC-ACC-AAT-GTG-GGX-TCX-AAA -GCC-XTC, wherein X is either I or C and Y is either G or A.
Claim 10
Dependent Claim
39 words
A DNA sequence including 37 codons that encode a 37-amino acid residue CGRP peptide sequence, wherein said 37-codon nucleotide sequence is as follows: GCC-TGX-YZC-ACT-GCC-ACC-TGC-GTG-ACC-CAT-CGG-CTG-GCG-GGC-TTG-CTG-AGC-AGA-TCA-GGG-GGT-GTG-GTG-AAG-AAC-AAC-TTT-GTG-CCC-ACC-AAT-GTG-GGX-TCX-AAA -GCC-XTC, wherein X is either I or C and Y is either G or A.
Claim 11
Dependent Claim
13 words
A DNA sequence according to claim 8 having the following sequence: GCC-TGX-GAC-ACT-GCC-ACC-TGX-GTG-ACX-CAT-CGG-CTG-GCG-GGC-TTG-CTG-AGC-AGA-TCA-GGG-GGT-GTG-GTG-AAG-AAC-AAC-TTT-GTG-CCC-ACC-AAT-GTG-GGX-TCX-AAA -GCC-TTC
Claim 12
Dependent Claim
13 words
A DNA sequency according to claim 8 having the following sequence: GCC-TGT-GAC-ACT-GCC-ACC-TGC-GTG-ACI-CAT-CGG-CTG-GCG-GGC-TTG-CTG-AGC-AGA-TCA-GGG-GGT-GTG-GTG-AAG-AAC-AAC-TTT-GTG-CCC-ACC-AAT-GTG-GGT-TCC-AAA -GCC-TTC.
Claim 13
Dependent Claim
13 words
A DNA sequence according to claim 8 having the following sequence; GCC-TGC-AAC-ACT-GCC-ACC-TGC-GTG-ACC-CAT-CGG-CTG-GCG-GGC-TTG-CTG-AGC-AGA-TCA-GGG-GGT-GTG-GTG-AAG-AAC-AAC-TTT-GTG-CCC-ACC-AAT-GTG-GGC-TCT-AAA -GCC-CTC.
Claim 14
Dependent Claim
13 words
A DNA dequence according to claim 8 having the following sequence: GCC-TGC-GAC-ACT-GCC-ACC-TGC-GTG-ACC-CAT-CGG-CTG-GCG-GGC-TTG-CTG-AGC-AGA-TCA-GGG-GGT-GTG-GTG-AAG-AAC-AAC-TTT-GTG-CCC-ACC-AAT-GTG-GGC-TCT-AAA -GCC-CTC.
Claim 15
Dependent Claim
13 words
A DNA sequence according to claim 8 having the following sequence: GCC-TGC-AAC-ACT-GCC-ACC-TGC-GTG-ACC-CAT-CGG-CTG-GCG-GGC-TTG-CTG-AGC-AGA-TCA-GGG-GGT-GTG-GTG-AAG-AAC-AAC-TTT-GTG-CCC-ACC-AAT-GTG-GGC-TCT-AAA -GCC-TTC.
References Cited (Backward Citations)
US4588684A
Cited
US4549986A
Cited
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