US4740306A
Expired / Lapsed
US
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Chromatographic column
Assignee: Temple University
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Filing: 1986-03-17
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Granted: 1988-04-26
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Expires: 2006-03-17
Abstract
Abstract A chromatographic column and process for isolating or purifying steroid hormone or cell membrane receptors using the column are provided in which the column contains at least three resin layers between the inlet and the outlet ends of the column, the layer closest the inlet being a strong cationic exchange resin, the middle layer being a matrix containing a triazine dye that will bind proteases and proteins with dinucleotide fold conformations, and the layer closest the outlet being a weak anionic exchange resin, the ratio of the volumes of the resin layers being 1:2:1.
Inventors
- 👤 Gerald Litwack
- 👤 Ted M. Kirsch
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 06:00
CPC Classifications
B; B01; B01J; B01J39/00; B01J39/26; G; G01; G01N; G01N30/00; G01N30/02; G01N30/50; G01N30/52; G01N30/60; Y; Y10; Y10S; Y10S435/00; Y10S435/803; Y10S435/814; Y10S435/815
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
74 words
A chromatographic column having at least three resin layers between the inlet and the outlet ends of the column, the layer closest the inlet being a strong cationic exchange resin, the middle layer being a backbone containing a triazine dye that will bind proteases and proteins with dinucleotide fold conformations, and the layer closest to the outlet being a weak anionic exchange resin, the ratio of the volumes of the resin layers being 1:2:1.
Claim 2
Dependent Claim
74 words
A chromatographic column having at least three resin layers between the inlet and the outlet ends of the column, the layer closest the inlet being a strong cationic exchange resin, the middle layer being a backbone containing a triazine dye that will bind proteases and proteins with dinucleotide fold conformations, and the layer closest to the outlet being a weak anionic exchange resin, the ratio of the volumes of the resin layers being 1:2:1.
Claim 3
Dependent Claim
24 words
The chromatographic column of claim 1 wherein the resin layer closest to the inlet is a six percent phosphorylated agarose gel crosslinked with epichlorohydrin.
Claim 4
Dependent Claim
17 words
The chromatographic column of claim 1 wherein the middle resin layer is a monochlorotriazine dye-containing synthetic matrix.
Claim 5
Dependent Claim
17 words
The chromatographic column of claim 3 wherein the matrix is a free radical polymerization product of N-acryloyl-2-amino-2-hydroxymethyl-1,3-propanediol.
Claim 6
Dependent Claim
19 words
The chromatographic column of claim 1 wherein the resin layer closest to the outlet is a diethylaminoethyl-containing synthetic matrix.
Claim 7
Dependent Claim
17 words
The chromatographic column of claim 4 wherein the matrix is a free radical polymerization product of N-acryloyl-2-amino-2-hydroxymethyl-1,3-propanediol.
Claim 8
Dependent Claim
18 words
The chromatographic column of claim 1 wherein the ratio of the volumes of the resin layers is 20:40:20.
Claim 9
Dependent Claim
84 words
A construction for rapid one-step isolation and purification of activated and unactivated forms of steroid hormone and cell membrane receptors which comprises a chromatographic column containing a matrix of resin layers between the inlet and the outlet, the resin layer closest to the inlet being a strong cationic exchange resin, the middle layer being a triazine dye containing exchange resin and the layer closest to the outlet being a weak anionic exchange resin, the ratio of the volumes of the resin layers being 1:2:1.
Claim 10
Dependent Claim
84 words
A construction for rapid one-step isolation and purification of activated and unactivated forms of steroid hormone and cell membrane receptors which comprises a chromatographic column containing a matrix of resin layers between the inlet and the outlet, the resin layer closest to the inlet being a strong cationic exchange resin, the middle layer being a triazine dye containing exchange resin and the layer closest to the outlet being a weak anionic exchange resin, the ratio of the volumes of the resin layers being 1:2:1.
Claim 11
Dependent Claim
23 words
The construction of claim 8 wherein the resin layer closest to the inlet is a six percent phosphorylated agarose gel crosslinked with epichlorohydrin.
Claim 12
Dependent Claim
16 words
The construction of claim 8 wherein the middle resin layer is a monochlorotriazine dye-containing synthetic matrix.
Claim 13
Dependent Claim
16 words
The construction of claim 10 wherein the matrix is a free radical polymerization product of N-acryloyl-2-amino-2-hydroxymethyl-1,3-propanediol.
Claim 14
Dependent Claim
18 words
The construction of claim 8 wherein the resin layer closest to the outlet is a diethylaminoethyl-containing synthetic matrix.
Claim 15
Dependent Claim
16 words
The construction of claim 12 wherein the matrix is a free radical polymerization product of N-acryloyl-2-amino-2-hydroxymethyl-1,3-propanediol.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-04-26 | 1991-10-26 | 1992-04-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-04-26 | 1995-10-26 | 1996-04-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-04-26 | 1999-10-26 | 2000-04-26 | $7700.00 | Window Closed / Lapsed |